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Q9PY82 (MUNS_REOVJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein mu-NS

Short name=MuNS
Gene names
Name:M3
OrganismReovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2) [Complete proteome]
Taxonomic identifier10885 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostMammalia [TaxID: 40674]

Protein attributes

Sequence length721 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Non-structural protein with ssRNA-binding activity. Is probably involved in the formation of viral inclusions, where the assembly of cores and the replication of viral RNA are thought to occur. Together with mu-2, recruits the other core proteins to these inclusions By similarity.

Subunit structure

Interacts with mu-2, sigma-NS, inner capsid proteins lambda-1 and sigma-2, and outer capsid protein lambda-2; in viral inclusions By similarity.

Post-translational modification

The N-terminus is blocked By similarity.

Sequence similarities

Belongs to the orthoreovirus mu-NS protein family.

Ontologies

Keywords
   Coding sequence diversityAlternative initiation
   DomainCoiled coil
   LigandRNA-binding
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform mu-NS (identifier: Q9PY82-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform mu-NSC (identifier: Q9PY82-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: Missing.
Note: It is unsure whether Met-41 is the initiator.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 721721Protein mu-NS
PRO_0000345002

Regions

Region1 – 1313Interaction with sigma-NS By similarity
Region14 – 4027Interaction with mu-2 By similarity
Coiled coil523 – 55634 Potential
Coiled coil632 – 68655 Potential

Natural variations

Alternative sequence1 – 4040Missing in isoform mu-NSC.
VSP_034869

Sequences

Sequence LengthMass (Da)Tools
Isoform mu-NS [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 0C08763E6E278153

FASTA72180,505
        10         20         30         40         50         60 
MASFKGFSAN TVPVSKTRKD TSSLTATPGL RAPSMSSPVD MAQSREFLTK AIEHGSMSIP 

        70         80         90        100        110        120 
YQHVNVPKVD RKVVSLVVRP FSAGAFSISG VISPAHAYLL ECLPQLEQAM AFVASPEAFQ 

       130        140        150        160        170        180 
ASDVAKRFTI KPGMSLQDAI TAFINFVSAM LKMTVTRQNF DVIIAEIERL ASSGVVNRTE 

       190        200        210        220        230        240 
EAKVADEELM LFGLDHRAPQ QIDVSEPVGI SRAVEIQTTN NVHLAPGLGN IDPEIYNEGR 

       250        260        270        280        290        300 
FMFMQHKPLA ADQSYFTTET ADYFKIYPTY DEHDGRMVDQ KQSGLILCTK DEVLAEQTIF 

       310        320        330        340        350        360 
KLDVPDDKTV HLLDRDDDHV VARFTRVFIE DVAPSHHAAQ RSNQRSLLDD LYANTQVVSV 

       370        380        390        400        410        420 
TPSALRWVIK HGVSDGIVNR KNVKICVGFD PLYTLATSNG LSLCSILMDE KLSVLNSACK 

       430        440        450        460        470        480 
MTLRSLLKTH RDLDLHRAFQ RVISQSYASL MCYYHPSRKL AYGELLFMSS QSDTVDGIKL 

       490        500        510        520        530        540 
QLDASRQCHE CPLLQQKIVE LEKHLIVQKS ASSDPTPVAL QPLLSQLREL SSEVTRLQMD 

       550        560        570        580        590        600 
LSRTQAINTR LEADVKSAQS CSLDMYLKHH TCINSHVKED ELMDAVRIAP DVRQELMLKR 

       610        620        630        640        650        660 
KATRQEWWER IARETSTTFQ SKIDELTLMN GKQAHEISEL RDSVTNYEKQ VAELVSTITQ 

       670        680        690        700        710        720 
NQTTYQQELQ ALVAKNIELD ALNQRQAKSV RITSSLLSAT PIDAVDGASD LIDFSVPADE 


L 

« Hide

Isoform mu-NSC [UniParc].

Checksum: 9BC36D73C106E79B
Show »

FASTA68176,394

References

[1]"Mammalian reovirus M3 gene sequences and conservation of coiled-coil motifs near the carboxyl terminus of the microNS protein."
McCutcheon A.M., Broering T.J., Nibert M.L.
Virology 264:16-24(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF174383 mRNA. Translation: AAF13170.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameMUNS_REOVJ
AccessionPrimary (citable) accession number: Q9PY82
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: May 1, 2000
Last modified: April 3, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families