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Protein

Protein mu-NS

Gene

M3

Organism
Reovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Non-structural protein with ssRNA-binding activity. Is probably involved in the formation of viral inclusions, where the assembly of cores and the replication of viral RNA are thought to occur. Together with mu-2, recruits the other core proteins to these inclusions (By similarity).By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein mu-NS
Short name:
MuNS
Gene namesi
Name:M3
OrganismiReovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2)
Taxonomic identifieri10885 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostiMammalia [TaxID: 40674]
ProteomesiUP000006370 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 721721Protein mu-NSPRO_0000345002Add
BLAST

Post-translational modificationi

The N-terminus is blocked.By similarity

Interactioni

Subunit structurei

Interacts with mu-2, sigma-NS, inner capsid proteins lambda-1 and sigma-2, and outer capsid protein lambda-2; in viral inclusions.By similarity

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 1313Interaction with sigma-NSBy similarityAdd
BLAST
Regioni14 – 4027Interaction with mu-2By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili523 – 55634Sequence AnalysisAdd
BLAST
Coiled coili632 – 68655Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the orthoreovirus mu-NS protein family.Curated

Keywords - Domaini

Coiled coil

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform mu-NS (identifier: Q9PY82-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASFKGFSAN TVPVSKTRKD TSSLTATPGL RAPSMSSPVD MAQSREFLTK
60 70 80 90 100
AIEHGSMSIP YQHVNVPKVD RKVVSLVVRP FSAGAFSISG VISPAHAYLL
110 120 130 140 150
ECLPQLEQAM AFVASPEAFQ ASDVAKRFTI KPGMSLQDAI TAFINFVSAM
160 170 180 190 200
LKMTVTRQNF DVIIAEIERL ASSGVVNRTE EAKVADEELM LFGLDHRAPQ
210 220 230 240 250
QIDVSEPVGI SRAVEIQTTN NVHLAPGLGN IDPEIYNEGR FMFMQHKPLA
260 270 280 290 300
ADQSYFTTET ADYFKIYPTY DEHDGRMVDQ KQSGLILCTK DEVLAEQTIF
310 320 330 340 350
KLDVPDDKTV HLLDRDDDHV VARFTRVFIE DVAPSHHAAQ RSNQRSLLDD
360 370 380 390 400
LYANTQVVSV TPSALRWVIK HGVSDGIVNR KNVKICVGFD PLYTLATSNG
410 420 430 440 450
LSLCSILMDE KLSVLNSACK MTLRSLLKTH RDLDLHRAFQ RVISQSYASL
460 470 480 490 500
MCYYHPSRKL AYGELLFMSS QSDTVDGIKL QLDASRQCHE CPLLQQKIVE
510 520 530 540 550
LEKHLIVQKS ASSDPTPVAL QPLLSQLREL SSEVTRLQMD LSRTQAINTR
560 570 580 590 600
LEADVKSAQS CSLDMYLKHH TCINSHVKED ELMDAVRIAP DVRQELMLKR
610 620 630 640 650
KATRQEWWER IARETSTTFQ SKIDELTLMN GKQAHEISEL RDSVTNYEKQ
660 670 680 690 700
VAELVSTITQ NQTTYQQELQ ALVAKNIELD ALNQRQAKSV RITSSLLSAT
710 720
PIDAVDGASD LIDFSVPADE L
Length:721
Mass (Da):80,505
Last modified:May 1, 2000 - v1
Checksum:i0C08763E6E278153
GO
Isoform mu-NSC (identifier: Q9PY82-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: Missing.

Note: It is unsure whether Met-41 is the initiator.
Show »
Length:681
Mass (Da):76,394
Checksum:i9BC36D73C106E79B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4040Missing in isoform mu-NSC. CuratedVSP_034869Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF174383 mRNA. Translation: AAF13170.1.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF174383 mRNA. Translation: AAF13170.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Mammalian reovirus M3 gene sequences and conservation of coiled-coil motifs near the carboxyl terminus of the microNS protein."
    McCutcheon A.M., Broering T.J., Nibert M.L.
    Virology 264:16-24(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.

Entry informationi

Entry nameiMUNS_REOVJ
AccessioniPrimary (citable) accession number: Q9PY82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: May 1, 2000
Last modified: January 7, 2015
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.