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Q9PY82

- MUNS_REOVJ

UniProt

Q9PY82 - MUNS_REOVJ

Protein

Protein mu-NS

Gene

M3

Organism
Reovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Non-structural protein with ssRNA-binding activity. Is probably involved in the formation of viral inclusions, where the assembly of cores and the replication of viral RNA are thought to occur. Together with mu-2, recruits the other core proteins to these inclusions By similarity.By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein mu-NS
    Short name:
    MuNS
    Gene namesi
    Name:M3
    OrganismiReovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2)
    Taxonomic identifieri10885 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
    Virus hostiMammalia [TaxID: 40674]
    ProteomesiUP000006370: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 721721Protein mu-NSPRO_0000345002Add
    BLAST

    Post-translational modificationi

    The N-terminus is blocked.By similarity

    Interactioni

    Subunit structurei

    Interacts with mu-2, sigma-NS, inner capsid proteins lambda-1 and sigma-2, and outer capsid protein lambda-2; in viral inclusions.By similarity

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 1313Interaction with sigma-NSBy similarityAdd
    BLAST
    Regioni14 – 4027Interaction with mu-2By similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili523 – 55634Sequence AnalysisAdd
    BLAST
    Coiled coili632 – 68655Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the orthoreovirus mu-NS protein family.Curated

    Keywords - Domaini

    Coiled coil

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative initiation. Align

    Isoform mu-NS (identifier: Q9PY82-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MASFKGFSAN TVPVSKTRKD TSSLTATPGL RAPSMSSPVD MAQSREFLTK    50
    AIEHGSMSIP YQHVNVPKVD RKVVSLVVRP FSAGAFSISG VISPAHAYLL 100
    ECLPQLEQAM AFVASPEAFQ ASDVAKRFTI KPGMSLQDAI TAFINFVSAM 150
    LKMTVTRQNF DVIIAEIERL ASSGVVNRTE EAKVADEELM LFGLDHRAPQ 200
    QIDVSEPVGI SRAVEIQTTN NVHLAPGLGN IDPEIYNEGR FMFMQHKPLA 250
    ADQSYFTTET ADYFKIYPTY DEHDGRMVDQ KQSGLILCTK DEVLAEQTIF 300
    KLDVPDDKTV HLLDRDDDHV VARFTRVFIE DVAPSHHAAQ RSNQRSLLDD 350
    LYANTQVVSV TPSALRWVIK HGVSDGIVNR KNVKICVGFD PLYTLATSNG 400
    LSLCSILMDE KLSVLNSACK MTLRSLLKTH RDLDLHRAFQ RVISQSYASL 450
    MCYYHPSRKL AYGELLFMSS QSDTVDGIKL QLDASRQCHE CPLLQQKIVE 500
    LEKHLIVQKS ASSDPTPVAL QPLLSQLREL SSEVTRLQMD LSRTQAINTR 550
    LEADVKSAQS CSLDMYLKHH TCINSHVKED ELMDAVRIAP DVRQELMLKR 600
    KATRQEWWER IARETSTTFQ SKIDELTLMN GKQAHEISEL RDSVTNYEKQ 650
    VAELVSTITQ NQTTYQQELQ ALVAKNIELD ALNQRQAKSV RITSSLLSAT 700
    PIDAVDGASD LIDFSVPADE L 721
    Length:721
    Mass (Da):80,505
    Last modified:May 1, 2000 - v1
    Checksum:i0C08763E6E278153
    GO
    Isoform mu-NSC (identifier: Q9PY82-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-40: Missing.

    Note: It is unsure whether Met-41 is the initiator.

    Show »
    Length:681
    Mass (Da):76,394
    Checksum:i9BC36D73C106E79B
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 4040Missing in isoform mu-NSC. CuratedVSP_034869Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF174383 mRNA. Translation: AAF13170.1.

    Keywords - Coding sequence diversityi

    Alternative initiation

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF174383 mRNA. Translation: AAF13170.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Mammalian reovirus M3 gene sequences and conservation of coiled-coil motifs near the carboxyl terminus of the microNS protein."
      McCutcheon A.M., Broering T.J., Nibert M.L.
      Virology 264:16-24(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.

    Entry informationi

    Entry nameiMUNS_REOVJ
    AccessioniPrimary (citable) accession number: Q9PY82
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3