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Protein

G-protein coupled receptor family C group 6 member A

Gene

gprc6a

Organism
Carassius auratus (Goldfish)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Olfactory receptor that is activated by amino acids that act as potent odorants in fish. Most highly activated by basic amino acids such as L-lysine and L-arginine.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei388 – 3881L-lysine 6-amino
Sitei389 – 3891Important for basic amino acid selectivity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Olfaction, Sensory transduction

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein coupled receptor family C group 6 member A
Alternative name(s):
Odorant receptor 5.24
Gene namesi
Name:gprc6a
OrganismiCarassius auratus (Goldfish)
Taxonomic identifieri7957 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeCarassius

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 567543ExtracellularSequence analysisAdd
BLAST
Transmembranei568 – 58821HelicalSequence analysisAdd
BLAST
Topological domaini589 – 60315CytoplasmicSequence analysisAdd
BLAST
Transmembranei604 – 62421HelicalSequence analysisAdd
BLAST
Topological domaini625 – 63511ExtracellularSequence analysisAdd
BLAST
Transmembranei636 – 65621HelicalSequence analysisAdd
BLAST
Topological domaini657 – 67620CytoplasmicSequence analysisAdd
BLAST
Transmembranei677 – 69721HelicalSequence analysisAdd
BLAST
Topological domaini698 – 72023ExtracellularSequence analysisAdd
BLAST
Transmembranei721 – 74121HelicalSequence analysisAdd
BLAST
Topological domaini742 – 75514CytoplasmicSequence analysisAdd
BLAST
Transmembranei756 – 77621HelicalSequence analysisAdd
BLAST
Topological domaini777 – 7826ExtracellularSequence analysis
Transmembranei783 – 80321HelicalSequence analysisAdd
BLAST
Topological domaini804 – 87774CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi47 – 471E → L or K: Induces a mild reduction in ligand-affinity. 1 Publication
Mutagenesisi74 – 741K → A: No effect. 1 Publication
Mutagenesisi78 – 781Q → A: Induces reduction in ligand-affinity. 1 Publication
Mutagenesisi111 – 1111S → A: No effect. 1 Publication
Mutagenesisi150 – 1501S → A: No effect.
Mutagenesisi151 – 1511S → A: No effect. 1 Publication
Mutagenesisi152 – 1521S → A: Induces reduction in ligand-affinity. 2 Publications
Mutagenesisi175 – 1751T → A or S: Almost abolishes L-Lys- and L-Arg-affinity. 2 Publications
Mutagenesisi190 – 1901R → A: Induces reduction in ligand-affinity. 1 Publication
Mutagenesisi195 – 1951D → A: Abolishes L-Lys-affinity. 1 Publication
Mutagenesisi223 – 2231Y → A: Abolishes L-Lys-affinity and strongly reduces L-Arg-affinity. 2 Publications
Mutagenesisi223 – 2231Y → H or F: Almost abolishes L-Arg-affinity. 2 Publications
Mutagenesisi283 – 2831K → A: Abolishes L-Lys-affinity. 1 Publication
Mutagenesisi284 – 2841S → A: No effect. 1 Publication
Mutagenesisi285 – 2851S → A: No effect. 1 Publication
Mutagenesisi309 – 3091D → A: Almost abolishes L-Lys-affinity. 2 Publications
Mutagenesisi309 – 3091D → L: Almost abolishes L-Arg-affinity. 2 Publications
Mutagenesisi388 – 3881D → A: Induces a 26-fold reduction in L-Lys-affinity without affecting affinity for L-Gln. 2 Publications
Mutagenesisi389 – 3891M → K: Induces a 24-fold reduction in L-Lys-affinity but increases affinity for L-Glu. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 877853G-protein coupled receptor family C group 6 member APRO_0000043200Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi53 – 531N-linked (GlcNAc...)Sequence analysis
Glycosylationi99 – 991N-linked (GlcNAc...)Sequence analysis
Glycosylationi135 – 1351N-linked (GlcNAc...)Sequence analysis
Glycosylationi263 – 2631N-linked (GlcNAc...)Sequence analysis
Glycosylationi310 – 3101N-linked (GlcNAc...)Sequence analysis
Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence analysis
Glycosylationi338 – 3381N-linked (GlcNAc...)Sequence analysis
Glycosylationi358 – 3581N-linked (GlcNAc...)Sequence analysis
Glycosylationi430 – 4301N-linked (GlcNAc...)Sequence analysis
Glycosylationi475 – 4751N-linked (GlcNAc...)Sequence analysis
Glycosylationi484 – 4841N-linked (GlcNAc...)Sequence analysis
Glycosylationi528 – 5281N-linked (GlcNAc...)Sequence analysis
Glycosylationi548 – 5481N-linked (GlcNAc...)Sequence analysis
Glycosylationi628 – 6281N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

N-glycosylated.

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed in olfactory epithelium. Also expressed in gills, tongue, lips and palatal organ. Not expressed in brain, kidney, liver, muscle, intestine, ovary and skin. In olfactory epithelium, it is widely expressed over the apical and medial portions of the olfactory sensory neurons, regions that contain olfactory neurons. Expressed in external epithelia, which contains taste buds and solitary chemosensory cells. On gill rakers, it is widely expressed in the surface epithelium, but excluded from taste buds.1 Publication

Interactioni

Subunit structurei

Homodimer; disulfide-linked.Curated

Structurei

3D structure databases

ProteinModelPortaliQ9PW88.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG096042.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9PW88-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGLDLSLVL MLSVLAGVRE VSLTQVNQQG VIAPGDIIIG GLFPIHEAAE
60 70 80 90 100
AVNFTGLNSF SSFQHPVCNR YYTKGLNQAL AMIHAVEMAN QSPMLSSLNL
110 120 130 140 150
TLGYRIYDTC SDVTTALWAV QDLTRPYSYC DSQTNSSQPV QPIMAVIGPS
160 170 180 190 200
SSEISIAVAR ELNLLMIPQI SYASTATILS DKSRFPAFMR TVPNDEYQTH
210 220 230 240 250
AMVQLLKDNK WTWVGIIITD GDYGRSAMES FVKHTEREGI CVAFKVILPD
260 270 280 290 300
SLADEQKLNI HINETVDIIE KNTKVNVVVS FAKSSQMKLL YEGLRSRNVP
310 320 330 340 350
KNKVWVASDN WSTSKNILKD VNLSDIGNIL GFTFKSGNVT AFLQYLKDLK
360 370 380 390 400
FGSEAKMNNS FLEEFLKLPE IGNAANAVQE QIKNTHLDMV FSVQMAVSAI
410 420 430 440 450
AKAVVELCVE RQCKTPSAIQ PWELLKQLRN VTFEKEGVMY NFDANGDINL
460 470 480 490 500
GYDVCLWDDD ESEKNDIIAE YYPSNSSFTF TRKNLSNIEN VLSKCSDSCQ
510 520 530 540 550
PGEYKKTAEG QHTCCYECLA CAENQYSNHT DADTCSKCDT ESLWSNANSS
560 570 580 590 600
KCYPKFYEYF EWNSGFAIAL LTLAALGILL LISMSALFFW QRNSLVVKAA
610 620 630 640 650
GGPLCHLILF SLLGSFISVI FFVGEPSNES CRVRQVIFGL SFTLCVSCIL
660 670 680 690 700
VKSLKILLAF QMNLELKELL RKLYKPYVIV CMCMGLQVTI CTLWLTLHRP
710 720 730 740 750
FIEKVVQPKS ILLECNEGSD LMFGLMLGYI VLLALICFTF AYKGRKLPQK
760 770 780 790 800
YNEAKFITFG MLIYLMAWVI FIPVHVTTSG KYVPAVEVVV ILISNYGILS
810 820 830 840 850
CHFLPKCYII IFKKEYNTKD AFLKNVFEYA RKSSENIRGL SGTDPHSKTD
860 870
NSVYVISNPS LVPEEKQVSV PEIDNVL
Length:877
Mass (Da):98,120
Last modified:May 1, 2000 - v1
Checksum:i5359109FD4A8A887
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF158963 mRNA. Translation: AAD46570.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF158963 mRNA. Translation: AAD46570.2.

3D structure databases

ProteinModelPortaliQ9PW88.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

GPCRDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG096042.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Functional identification of a goldfish odorant receptor."
    Speca D.J., Lin D.M., Sorensen P.W., Isacoff E.Y., Ngai J., Dittman A.H.
    Neuron 23:487-498(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
  2. "Molecular similarities in the ligand binding pockets of an odorant receptor and the metabotropic glutamate receptors."
    Kuang D., Yao Y., Wang M., Pattabiraman N., Kotra L.P., Hampson D.R.
    J. Biol. Chem. 278:42551-42559(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF LYS-74; GLN-78; SER-111; SER-111; SER-151; SER-152; THR-175; ARG-190; ASP-195; TYR-223; LYS-283; SER-284; SER-285; ASP-309 AND ASP-388.
  3. "Molecular determinants of ligand selectivity in a vertebrate odorant receptor."
    Luu P., Acher F., Bertrand H.-O., Fan J., Ngai J.
    J. Neurosci. 24:10128-10137(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF GLU-47; SER-152; THR-175; TYR-223; ASP-309; ASP-388 AND MET-389.

Entry informationi

Entry nameiGPC6A_CARAU
AccessioniPrimary (citable) accession number: Q9PW88
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: May 1, 2000
Last modified: October 1, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.