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Protein

Zinc metalloproteinase/disintegrin

Gene
N/A
Organism
Gloydius brevicaudus (Korean slamosa snake) (Agkistrodon halys brevicaudus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Snake venom metalloproteinase Mt-d: this recombinant protein hydrolyzes fibronectin, but has no effect on type I gelatin and type I to V collagens. Selectively hydrolyzes the Aalpha-chain of fibrinogen (FGA), but has no effect on fibrin.1 Publication
Disintegrin: inhibits ADP-induced platelet aggregation.By similarity
Recombinant metalloproteinase-disintegrin Mt-d-I (393-408): hydrolyzes type I gelatin, type III and V collagens, but has no effect on type I, II, IV collagens and fibronectin. Selectively hydrolyzes the Aalpha-chain of fibrinogen, but has no effect on fibrin. May induce hemorrhage in vascular tissue. Strongly inhibits ADP-induced platelet aggregation. When concentrated, Mt-d-I undergoes autoproteolytic processing into metalloproteinase and disintegrin.1 Publication

Miscellaneous

The disintegrin belongs to the dimeric disintegrin subfamily.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi200CalciumBy similarity1
Metal bindingi284CalciumBy similarity1
Metal bindingi333Zinc; catalyticBy similarity1
Active sitei334PROSITE-ProRule annotation1
Metal bindingi337Zinc; catalyticBy similarity1
Metal bindingi343Zinc; catalyticBy similarity1
Metal bindingi388Calcium; via carbonyl oxygenBy similarity1
Metal bindingi391CalciumBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionCell adhesion impairing toxin, Fibrinogenolytic toxin, Hemostasis impairing toxin, Hydrolase, Metalloprotease, Platelet aggregation inhibiting toxin, Protease, Toxin
LigandCalcium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase/disintegrin
Cleaved into the following 2 chains:
OrganismiGloydius brevicaudus (Korean slamosa snake) (Agkistrodon halys brevicaudus)
Taxonomic identifieri259325 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeGloydius

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000034029321 – 189By similarityAdd BLAST169
ChainiPRO_0000340294190 – 393Snake venom metalloproteinase Mt-dAdd BLAST204
PropeptideiPRO_0000340295394 – 409By similarityAdd BLAST16
ChainiPRO_0000340296410 – 482DisintegrinAdd BLAST73

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi308 ↔ 388By similarity
Disulfide bondi348 ↔ 372By similarity
Disulfide bondi424 ↔ 447By similarity
Disulfide bondi438 ↔ 444By similarity
Disulfide bondi443 ↔ 468By similarity
Disulfide bondi456 ↔ 475By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Homodimer; disulfide-linked (disintegrin).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9PVK9.
SMRiQ9PVK9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini197 – 393Peptidase M12BPROSITE-ProRule annotationAdd BLAST197
Domaini401 – 482DisintegrinPROSITE-ProRule annotationAdd BLAST82

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi460 – 462Cell attachment site3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006978.

Family and domain databases

CDDicd04269. ZnMc_adamalysin_II_like. 1 hit.
Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiView protein in InterPro
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR036436. Disintegrin_dom_sf.
IPR024079. MetalloPept_cat_dom_sf.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
IPR034027. Reprolysin_adamalysin.
PfamiView protein in Pfam
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PRINTSiPR00289. DISINTEGRIN.
SMARTiView protein in SMART
SM00050. DISIN. 1 hit.
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiView protein in PROSITE
PS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9PVK9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQVLLVTIC LAAFPYQGSS MILESGNVND YEVVYPQKVP ALPKGAVPQK
60 70 80 90 100
YEDAMQYEFK VNGEPVVLHL EKNKGLFSKD YSETHYSPDG RKITTNPPVE
110 120 130 140 150
DHCYYHGHIQ NDADSTASIS ACNGLKGHFK HQGEMYLIEP LKLSDSEAHA
160 170 180 190 200
VYKYENVEKE DEAPKMCGVT QTNWKSDEPI KASQLVVTAE QQRFPQRYIE
210 220 230 240 250
LVVVADHGMF TKYDSNLDTI TTWVHELVNN INEFYRSLNV RVSLTELEIW
260 270 280 290 300
SNQDLINVQS AAADTLEAFG DWRETDLLNR ISHDNAQLLT TIDLDGNTIG
310 320 330 340 350
LAHVGTMCDP KYSVGIVQDH SAINLLVAVT MAHELGHNLG MDHDGNQCHC
360 370 380 390 400
GANSSVMGDV LRVGVSYEFS DCNENEYQTY VTDHNPQCIL NEPLRTDTVS
410 420 430 440 450
TPVSGNELLE AGVECDCGAP ANPCCDAATC KLRPGAQCAE GLCCDQCRFM
460 470 480
KEGTICRMAR GDDMDDYCNG ISAGCPRNPF HA
Length:482
Mass (Da):53,410
Last modified:May 1, 2000 - v1
Checksum:iC6014BBE87BC8B15
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051789 mRNA. Translation: AAD02654.1.

Similar proteinsi

Entry informationi

Entry nameiVM2MD_GLOBR
AccessioniPrimary (citable) accession number: Q9PVK9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: May 1, 2000
Last modified: November 22, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families