Q9PVK5 (LDHA_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-lactate dehydrogenase A chain
Short name=LDH-A
EC=1.1.1.27

Gene names

Name:

ldha

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH.
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW response to hypoxia Inferred from direct assay. Source: ZFIN
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 333	332	L-lactate dehydrogenase A chain		PRO_0000168429
Regions
	Nucleotide binding	30 – 58	29	NAD By similarity
Sites
	Active site	194	1	Proton acceptor By similarity
	Binding site	100	1	NAD By similarity
	Binding site	107	1	Substrate By similarity
	Binding site	139	1	NAD or substrate By similarity
	Binding site	170	1	Substrate By similarity
	Binding site	249	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PVK5 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: B5A040A6DDC014BC	FASTA	333	36,246
	10 20 30 40 50 60 MASTKEKLIA HVSKEQPAGP TNKVTVVGVG MVGMAAAVSI LLKDLTDELA LVDVMEDKLK 70 80 90 100 110 120 GEAMDLQHGS LFLKTHKIVA DKDYSVTANS KVVVVTAGAR QQEGESRLNL VQRNVNIFKF 130 140 150 160 170 180 IIPNIIKYSP NCILLVVSNP VDILTYVAWK LSGLPRNRVI GSGTNLDSAR FRYLMGEKLG 190 200 210 220 230 240 IHPSSCHGWV VGEHGDSSVP VWSGVNVAGV SLQALNPDLG TDKDKEDWKS VHKMVVDSAY 250 260 270 280 290 300 EVIKLKGYTS WAIGMSVADL CESILKNMHK CHPVSTLVKG MHGVNEEVFL SVPCILGNNG 310 320 330 LTDVVHMTLK PEEEKQLVKS AETLWGVQKE LTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular evolution of vertebrate lactate dehydrogenase isozymes by gene duplication." Tsoi S.C.-M., Li J.Y., Mannen H., Li S.S.-L. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF067201 mRNA. Translation: AAF02212.1. BC067188 mRNA. Translation: AAH67188.1.
IPI	IPI00512269.
RefSeq	NP_571321.1. NM_131246.1.
UniGene	Dr.4212.
3D structure databases
ProteinModelPortal	Q9PVK5.
SMR	Q9PVK5. Positions 2-333.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9PVK5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000059886; ENSDARP00000059885; ENSDARG00000040856.
GeneID	30496.
KEGG	dre:30496.
Organism-specific databases
CTD	3939.
ZFIN	ZDB-GENE-991026-5. ldha.
Phylogenomic databases
GeneTree	ENSGT00550000074541.
HOGENOM	HBG566126.
HOVERGEN	HBG000462.
InParanoid	Q9PVK5.
OMA	IHPLSCH.
OrthoDB	EOG4RR6HR.
PhylomeDB	Q9PVK5.
Gene expression databases
ArrayExpress	Q9PVK5.
Bgee	Q9PVK5.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00016.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDHA_DANRE

Accession

Primary (citable) accession number: Q9PVK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 88 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program