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Reviewed, UniProtKB/Swiss-Prot Q9PVK5 (LDHA_DANRE)

Last modified June 16, 2009. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    L-lactate dehydrogenase A chain
      Short name=LDH-A
    EC=1.1.1.27
Gene names
Name: ldha
OrganismDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

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Protein attributes

Sequence length333 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Catalytic activity

(S)-lactate + NAD+ = pyruvate + NADH.

Pathway

Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the LDH/MDH superfamily. LDH family.

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Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological processanaerobic glycolysis

Inferred from electronic annotation. Source: InterPro

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

response to hypoxia

Inferred from direct assay. Source: ZFIN

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionL-lactate dehydrogenase activity

Inferred from electronic annotation. Source: EC

binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 333332L-lactate dehydrogenase A chain
PRO_0000168429

Regions

Nucleotide binding30 – 5829NAD By similarity

Sites

Active site1941Proton acceptor By similarity
Binding site1001NAD By similarity
Binding site1071Substrate By similarity
Binding site1391NAD or substrate By similarity
Binding site1701Substrate By similarity
Binding site2491Substrate By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q9PVK5-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: B5A040A6DDC014BC

FASTA33336,246
        10         20         30         40         50         60 
MASTKEKLIA HVSKEQPAGP TNKVTVVGVG MVGMAAAVSI LLKDLTDELA LVDVMEDKLK 

        70         80         90        100        110        120 
GEAMDLQHGS LFLKTHKIVA DKDYSVTANS KVVVVTAGAR QQEGESRLNL VQRNVNIFKF 

       130        140        150        160        170        180 
IIPNIIKYSP NCILLVVSNP VDILTYVAWK LSGLPRNRVI GSGTNLDSAR FRYLMGEKLG 

       190        200        210        220        230        240 
IHPSSCHGWV VGEHGDSSVP VWSGVNVAGV SLQALNPDLG TDKDKEDWKS VHKMVVDSAY 

       250        260        270        280        290        300 
EVIKLKGYTS WAIGMSVADL CESILKNMHK CHPVSTLVKG MHGVNEEVFL SVPCILGNNG 

       310        320        330 
LTDVVHMTLK PEEEKQLVKS AETLWGVQKE LTL

« Hide

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References

« Hide 'large scale' references
[1]"Molecular evolution of vertebrate lactate dehydrogenase isozymes by gene duplication."
Tsoi S.C.-M., Li J.Y., Mannen H., Li S.S.-L.
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.

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Cross-references

Sequence databases

AF067201 mRNA. Translation: AAF02212.1.
BC067188 mRNA. Translation: AAH67188.1.
IPIIPI00512269.
RefSeqNP_571321.1.
UniGeneDr.4212

3D structure databases

HSSPHSSP built from PDB template 1I10 based on UniProtKB P00338.
SMRQ9PVK5. Positions 2-333.
ModBaseSearch...

Genome annotation databases

EnsemblENSDARG00000040856. Danio rerio. [Contig view]
GeneID30496.
KEGGdre:30496.

Organism-specific databases

ZFINZDB-GENE-991026-5. ldha.

Phylogenomic databases

HOGENOMQ9PVK5.
HOVERGENQ9PVK5.
OMAQ9PVK5. VISFHCL.

Enzyme and pathway databases

BRENDA1.1.1.27. 96826.

Gene expression databases

BgeeQ9PVK5.

Family and domain databases

InterProIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR001236. Lactate/malate_DH.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
TIGRFAMsTIGR01771. L-LDH-NAD. 1 hit.
PROSITEPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameLDHA_DANRE
AccessionPrimary (citable) accession number: Q9PVK5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 65 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectZebrafish annotation project

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents