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Protein

L-lactate dehydrogenase A chain

Gene

ldha

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ldha), L-lactate dehydrogenase B-A chain (ldhba), L-lactate dehydrogenase (ldhbb), L-lactate dehydrogenase A chain (ldha), L-lactate dehydrogenase (ldhbb), L-lactate dehydrogenase B-B chain (ldhbb)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100NADBy similarity1
Binding sitei107SubstrateBy similarity1
Binding sitei139NAD or substrateBy similarity1
Binding sitei170SubstrateBy similarity1
Active sitei194Proton acceptorBy similarity1
Binding sitei249SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 58NADBy similarityAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-DRE-70268. Pyruvate metabolism.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase A chain (EC:1.1.1.27)
Short name:
LDH-A
Gene namesi
Name:ldha
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 25

Organism-specific databases

ZFINiZDB-GENE-991026-5. ldha.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001684292 – 333L-lactate dehydrogenase A chainAdd BLAST332

Proteomic databases

PaxDbiQ9PVK5.
PRIDEiQ9PVK5.

Expressioni

Gene expression databases

BgeeiENSDARG00000040856.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000059885.

Structurei

3D structure databases

ProteinModelPortaliQ9PVK5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00550000074541.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiQ9PVK5.
KOiK00016.
OMAiHLMGEKL.
OrthoDBiEOG091G0HME.
PhylomeDBiQ9PVK5.
TreeFamiTF314963.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9PVK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTKEKLIA HVSKEQPAGP TNKVTVVGVG MVGMAAAVSI LLKDLTDELA
60 70 80 90 100
LVDVMEDKLK GEAMDLQHGS LFLKTHKIVA DKDYSVTANS KVVVVTAGAR
110 120 130 140 150
QQEGESRLNL VQRNVNIFKF IIPNIIKYSP NCILLVVSNP VDILTYVAWK
160 170 180 190 200
LSGLPRNRVI GSGTNLDSAR FRYLMGEKLG IHPSSCHGWV VGEHGDSSVP
210 220 230 240 250
VWSGVNVAGV SLQALNPDLG TDKDKEDWKS VHKMVVDSAY EVIKLKGYTS
260 270 280 290 300
WAIGMSVADL CESILKNMHK CHPVSTLVKG MHGVNEEVFL SVPCILGNNG
310 320 330
LTDVVHMTLK PEEEKQLVKS AETLWGVQKE LTL
Length:333
Mass (Da):36,246
Last modified:January 23, 2007 - v3
Checksum:iB5A040A6DDC014BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067201 mRNA. Translation: AAF02212.1.
BC067188 mRNA. Translation: AAH67188.1.
RefSeqiNP_571321.1. NM_131246.1.
UniGeneiDr.4212.

Genome annotation databases

EnsembliENSDART00000163892; ENSDARP00000137433; ENSDARG00000101251.
ENSDART00000167542; ENSDARP00000140963; ENSDARG00000101251.
GeneIDi30496.
KEGGidre:30496.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067201 mRNA. Translation: AAF02212.1.
BC067188 mRNA. Translation: AAH67188.1.
RefSeqiNP_571321.1. NM_131246.1.
UniGeneiDr.4212.

3D structure databases

ProteinModelPortaliQ9PVK5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000059885.

Proteomic databases

PaxDbiQ9PVK5.
PRIDEiQ9PVK5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000163892; ENSDARP00000137433; ENSDARG00000101251.
ENSDART00000167542; ENSDARP00000140963; ENSDARG00000101251.
GeneIDi30496.
KEGGidre:30496.

Organism-specific databases

CTDi3939.
ZFINiZDB-GENE-991026-5. ldha.

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00550000074541.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiQ9PVK5.
KOiK00016.
OMAiHLMGEKL.
OrthoDBiEOG091G0HME.
PhylomeDBiQ9PVK5.
TreeFamiTF314963.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
ReactomeiR-DRE-70268. Pyruvate metabolism.

Miscellaneous databases

PROiQ9PVK5.

Gene expression databases

BgeeiENSDARG00000040856.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLDHA_DANRE
AccessioniPrimary (citable) accession number: Q9PVK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: January 23, 2007
Last modified: October 5, 2016
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.