Q9PVE8 (C330_FUNHE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 3A30
EC=1.14.14.1

Alternative name(s):
CYPIIIA30

Gene names

Name:

cyp3a30

Organism

Fundulus heteroclitus (Killifish) (Mummichog)

Taxonomic identifier

8078 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Euteleostei › Neognathi › Neoteleostei › Eurypterygii › Ctenosquamata › Acanthomorpha › Euacanthomorpha › Holacanthopterygii › Acanthopterygii › Euacanthopterygii › Percomorpha › Smegmamorpha › Atherinomorpha › Cyprinodontiformes › Cyprinodontoidei › Fundulidae › Fundulus

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Putative steroid 6-beta-hydroxylase.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein Potential. Microsome membrane; Peripheral membrane protein Potential.
Tissue specificity	Highly expressed in liver and intestine. Moderate expression in gill and spleen. Low expression in kidney, brain and heart. Ref.1
Sequence similarities	Belongs to the cytochrome P450 family.
Caution	Due to a recent gene duplication event, CYP3A30 and CYP3A56 are very similar. Because of this it was not possible to distinguish between the two genes when measuring the tissue expression.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Cytochrome P450 3A30		PRO_0000051809
Sites
	Metal binding	441	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PVE8 [UniParc]. Last modified August 22, 2003. Version 2. Checksum: C19FF4CE75AB37D4	FASTA	496	57,045
	10 20 30 40 50 60 MGYFYLTAET WTLLVAFVTL LLVYAYWPYG TFKRLGISGP KPVPFFGTML HYRRGFFTFD 70 80 90 100 110 120 EECKKKYGKV WGIYDGRQPV LCVTDPEIIK AVLVKECLSF FTNRRNFRLN GPLYDAVSIA 130 140 150 160 170 180 EDDQWKRIRS VLSPSFTSGR LKEMFEIMKN HSANLIRSMK KKADKDEPLD LKEFFGSYSM 190 200 210 220 230 240 DVVTSTAFSV DIDSLNNPSD PFVTNIKKML KFDFLNPLFL AVAFFPFLGP ILEKFELSFF 250 260 270 280 290 300 PKSVTDFFYA SLEKIKSNRE ASQQKSRVDF LQLMIDSQKN SGAQQDKSLT DHEILSQSMI 310 320 330 340 350 360 FIFAGYETSS SSLTFLAYNL ATNPEVMKKL QEEIDATFPN KAPVHYQPLM EMEYLDCVIN 370 380 390 400 410 420 ESLRLFPIAA RLERVAKAAV EINGVVIPKD MVVMIPTWPL HRDPEIWPEP EAFKPERFSK 430 440 450 460 470 480 KNKDNIDPYI YMPFGSGPRN CIGMRFALVL IKLAVVEILQ QYSFSVCKET EVPFEMDIQG 490 LLAPKRPIQL KLVPRS

References

[1]	"Hepatic versus extrahepatic expression of CYP3A30 and CYP3A56 in adult killifish (Fundulus heteroclitus)." Hegelund T., Celander M.C. Aquat. Toxicol. 64:277-291(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Intestine and Liver.
[2]	"Isolation of a cytochrome P450 3A cDNA sequence (CYP3A30) from the marine teleost Fundulus heteroclitus and phylogenetic analyses of CYP3A genes." Celander M.C., Stegeman J.J. Biochem. Biophys. Res. Commun. 236:306-312(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 312-435. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF105068 mRNA. Translation: AAF14117.2.
PIR	A58612.
3D structure databases
ProteinModelPortal	Q9PVE8.
SMR	Q9PVE8. Positions 29-495.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG108567.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR008072. Cyt_P450_E_CYP3A. IPR002402. Cyt_P450_E_grp-II. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00464. EP450II. PR01689. EP450IICYP3A. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C330_FUNHE

Accession

Primary (citable) accession number: Q9PVE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	August 22, 2003
Last modified:	April 3, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program