Q9PUH4 (PA210_AUSSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Phospholipase A2 isozyme S5-32M EC=3.1.1.4 Alternative name(s): ASPLA10 Phosphatidylcholine 2-acylhydrolase
Organism	Austrelaps superbus (Lowland copperhead snake) (Hoplocephalus superbus)
Taxonomic identifier	29156 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Elapidae › Acanthophiinae › Austrelaps

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Inhibits collagen-induced platelet aggregation By similarity.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Calcium Probable.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the phospholipase A2 family. Group I subfamily.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Toxin
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 27	8	Potential		PRO_0000022803
	Chain	28 – 146	119	Phospholipase A2 isozyme S5-32M		PRO_0000022804
Sites
	Active site	75	1	By similarity
	Active site	120	1	By similarity
	Metal binding	55	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	57	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	59	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	76	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	38 ↔ 98		By similarity
	Disulfide bond	54 ↔ 145		By similarity
	Disulfide bond	56 ↔ 72		By similarity
	Disulfide bond	71 ↔ 126		By similarity
	Disulfide bond	78 ↔ 119		By similarity
	Disulfide bond	87 ↔ 112		By similarity
	Disulfide bond	105 ↔ 117		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PUH4 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 9C00CAEACDFDA180	FASTA	146	16,379
	10 20 30 40 50 60 MYPAHLLVLL AVCVSLLGAA SIPPQPLNLV QFSYLIQCAN HGSRATWHYT DYGCYCGKGG 70 80 90 100 110 120 SGTPVDELDR CCKIHDDCYG EAEKKGCYPK MSAYDYYCGE NGPYCRNIKK ECQRFVCDCD 130 140 VEAAKCFARA PYNDANWNID TKKRCQ

References

[1]	"Phospholipase A(2) with platelet aggregation inhibitor activity from Austrelaps superbus venom: protein purification and cDNA cloning." Singh S.B., Armugam A., Kini R.M., Jeyaseelan K. Arch. Biochem. Biophys. 375:289-303(2000) [PubMed: 10700385] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Venom gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF184136 mRNA. Translation: AAD56559.1.
3D structure databases
ProteinModelPortal	Q9PUH4.
SMR	Q9PUH4. Positions 28-146.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG008137.
Family and domain databases
InterPro	IPR016090. PLipase_A2. IPR013090. PLipase_A2_AS. IPR001211. PLipase_A2_euk. [Graphical view]
Gene3D	G3DSA:1.20.90.10. Phospholipase_A2. 1 hit.
PANTHER	PTHR11716. Phospholipase_A2. 1 hit.
Pfam	PF00068. Phospholip_A2_1. 1 hit. [Graphical view]
PRINTS	PR00389. PHPHLIPASEA2.
SMART	SM00085. PA2c. 1 hit. [Graphical view]
SUPFAM	SSF48619. PhospholipaseA2. 1 hit.
PROSITE	PS00119. PA2_ASP. 1 hit. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA210_AUSSU

Accession

Primary (citable) accession number: Q9PUH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	May 1, 2000
Last modified:	October 19, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program

Annotation program

Chordata Protein Annotation Program