Q9PUE4 (COPG2_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coatomer subunit gamma-2

Alternative name(s):
Gamma-2-coat protein
Short name=Gamma-2-COP

Gene names

Name:	copg2
ORF Names:	si:ch211-285d14.8

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	873 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins By similarity.
Subunit structure	Oligomeric complex By similarity.
Subcellular location	Cytoplasm By similarity. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasmic vesicle › COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the COPG family. Contains 6 HEAT repeats.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Cytoplasmic vesicle Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular protein transport Inferred from electronic annotation. Source: InterPro vesicle-mediated transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	COPI vesicle coat Inferred from electronic annotation. Source: InterPro
Molecular_function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9PUE4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9PUE4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     296-299: CSSP → LLVR
     300-873: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 873	873	Coatomer subunit gamma-2		PRO_0000342519
Regions
	Repeat	64 – 101	38	HEAT 1
	Repeat	283 – 320	38	HEAT 2
	Repeat	321 – 355	35	HEAT 3
	Repeat	356 – 392	37	HEAT 4
	Repeat	395 – 430	36	HEAT 5
	Repeat	467 – 504	38	HEAT 6
Natural variations
	Alternative sequence	296 – 299	4	CSSP → LLVR in isoform 2.		VSP_034478
	Alternative sequence	300 – 873	574	Missing in isoform 2.		VSP_034479
Experimental info
	Sequence conflict	56	1	N → D in AAH45918. Ref.3
	Sequence conflict	103	1	I → V in AAH96860. Ref.3
	Sequence conflict	576	1	M → I in AAH96860. Ref.3
	Sequence conflict	649	1	C → F in AAF05718. Ref.1
	Sequence conflict	853	1	I → M in AAF05717. Ref.1
	Sequence conflict	853	1	I → M in AAF05718. Ref.1
	Sequence conflict	853	1	I → M in AAH96860. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 1, 2008. Version 2. Checksum: EE664350506B48FF	FASTA	873	97,529
	10 20 30 40 50 60 MIKKFDKKDE ESGSGSNPFQ HLEKSAVLQE ARIFNETPIN PRRCLHILTK IIYLLNQGEH 70 80 90 100 110 120 FGTTEATEAF FAMTRLFQSN DQTLRRMCYL TIKEMANISE DVIIVTSSLT KDMTGKEDVY 130 140 150 160 170 180 RGPAIRALCR ITDTTMLQAI ERYMKQAIVD KVPSVSSSAL VSSLHMVKMS FDVVKRWVNE 190 200 210 220 230 240 AQEAASSDNI MVQYHALGLL YHLRKNDRLA VTKMLNKFTK SGLKSPFAYC MMIRIASKLL 250 260 270 280 290 300 EETEGGHDSP LFDFIESCLR NKHEMVVYEA ASAIVHMPNC TARELAPAVS VLQLFCSSPK 310 320 330 340 350 360 AALRYAAVRT LNKVAMKHPS AVTACNLDLE NLITDSNRSI ATLAITTLLK TGSESSVDRL 370 380 390 400 410 420 MKQISSFVSE ISDEFKVVVV QAISALCQKY PRKHSVMMNF LSNMLRDDGG FEYKRAIVDC 430 440 450 460 470 480 IISIIEENPE SKETGLAHLC EFIEDCEHTV LATKILHLLG KEGPRTPTPS KYIRFIFNRV 490 500 510 520 530 540 VLESEAVRAA AVSALAKFGA QNDDLLPSVL VLMQRCMMDS DDEVRDRATF YMNVLQQKQK 550 560 570 580 590 600 ALNAAYIFNG LSVSVLGLEK SLHQYTLEPS EKPFDMKTVP LATAPITEHK TEIAPVATSK 610 620 630 640 650 660 LPEKLAPSRQ DIYQEQLSAI PEFQGLGPLF KSSEPVQLTE AETEYVVRCI KHTFANHMIF 670 680 690 700 710 720 QFDCTNTLND QLLQKVLVQM EPSESYEVLH YVPAANLPYS QPGSCYSLVR LPEDDPTAVS 730 740 750 760 770 780 CTFSCTMKYL VRDCDPNTGE PDDDGYDDEY VLEDLEVTVA DHIQKVLKPN FAAAWDEVGD 790 800 810 820 830 840 ECEKEETFAL ATVRTLDEAV NNIVSFLGMQ PCERSDKVPE NKNSHVLFLA GVFRGGHDVL 850 860 870 VRARLALADG VTIQVTVRST DDNVVDVILA SVG
	Isoform 2 [UniParc]. Checksum: 40BCF1795764DCC3	FASTA	299	33,908
	10 20 30 40 50 60 MIKKFDKKDE ESGSGSNPFQ HLEKSAVLQE ARIFNETPIN PRRCLHILTK IIYLLNQGEH 70 80 90 100 110 120 FGTTEATEAF FAMTRLFQSN DQTLRRMCYL TIKEMANISE DVIIVTSSLT KDMTGKEDVY 130 140 150 160 170 180 RGPAIRALCR ITDTTMLQAI ERYMKQAIVD KVPSVSSSAL VSSLHMVKMS FDVVKRWVNE 190 200 210 220 230 240 AQEAASSDNI MVQYHALGLL YHLRKNDRLA VTKMLNKFTK SGLKSPFAYC MMIRIASKLL 250 260 270 280 290 EETEGGHDSP LFDFIESCLR NKHEMVVYEA ASAIVHMPNC TARELAPAVS VLQLFLLVR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Duplication of genes encoding non-clathrin coat protein gamma-COP in vertebrate, insect and plant evolution." Hahn Y., Lee Y.J., Yun J.H., Yang S.K., Park C.W., Mita K., Huh T.-L., Rhee M., Chung J.H. FEBS Lett. 482:31-36(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	The Danio rerio sequencing project at the Sanger Institute Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[3]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: AB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF191561 mRNA. Translation: AAF05717.1. AF191562 mRNA. Translation: AAF05718.1. CR749763, BX004864 Genomic DNA. Translation: CAK04920.1. BX004864, CR749763 Genomic DNA. Translation: CAK05111.1. BC045918 mRNA. Translation: AAH45918.1. BC096860 mRNA. Translation: AAH96860.1.
IPI	IPI00495942. IPI00898490.
RefSeq	NP_571069.1. NM_130994.1.
UniGene	Dr.75573.
3D structure databases
HSSP	HSSP built from PDB template 1R4X based on UniProtKB Q9Y678.
ProteinModelPortal	Q9PUE4.
SMR	Q9PUE4. Positions 604-873.
ModBase	Search...
Proteomic databases
PRIDE	Q9PUE4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000043660; ENSDARP00000043659; ENSDARG00000034823.
GeneID	30187.
KEGG	dre:30187.
Organism-specific databases
CTD	26958.
ZFIN	ZDB-GENE-000208-8. copg2.
Phylogenomic databases
eggNOG	COG5240.
GeneTree	ENSGT00390000016313.
HOGENOM	HOG000184434.
HOVERGEN	HBG004368.
InParanoid	Q7ZVC5.
OrthoDB	EOG4DZ1TM.
Gene expression databases
Bgee	Q9PUE4.
Family and domain databases
Gene3D	1.25.10.10. 1 hit. 2.60.40.1480. 1 hit. 3.30.310.30. 1 hit.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR002553. Clathrin/coatomer_adapt-like_N. IPR015873. Clathrin_a/coatomer_app_sub_C. IPR009028. Coatomer/calthrin_app_sub_C. IPR013041. Coatomer/clathrin_app_Ig-like. IPR017106. Coatomer_gsu. IPR014863. Coatomer_gsu_app. IPR013040. Coatomer_gsu_app_Ig-like-sub. [Graphical view]
PANTHER	PTHR10261. PTHR10261. 1 hit.
Pfam	PF01602. Adaptin_N. 1 hit. PF08752. Gamma-COP. 1 hit. [Graphical view]
PIRSF	PIRSF037093. Coatomer_gamma_subunit. 1 hit.
SUPFAM	SSF55711. AP2_adap_app. 1 hit. SSF48371. ARM-type_fold. 1 hit. SSF49348. Clath_adapt. 1 hit.
PROSITE	PS50077. HEAT_REPEAT. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	20806653.

Entry information

Entry name

COPG2_DANRE

Accession

Primary (citable) accession number: Q9PUE4
Secondary accession number(s): Q1L8N9 , Q4V9K1, Q7ZVC5, Q9PUE3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 2008
Last sequence update:	July 1, 2008
Last modified:	April 3, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program