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Protein

Insulin-like growth factor-binding protein 2-A

Gene

igfbp2a

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.2 Publications

GO - Molecular functioni

  1. insulin-like growth factor I binding Source: UniProtKB
  2. insulin-like growth factor II binding Source: UniProtKB

GO - Biological processi

  1. angiogenesis Source: ZFIN
  2. growth Source: ZFIN
  3. heart development Source: ZFIN
  4. negative regulation of cell proliferation Source: UniProtKB
  5. negative regulation of developmental growth Source: UniProtKB
  6. negative regulation of DNA replication Source: UniProtKB
  7. regulation of cell growth Source: InterPro
  8. regulation of insulin-like growth factor receptor signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Growth regulation

Keywords - Ligandi

Growth factor binding

Enzyme and pathway databases

ReactomeiREACT_289688. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Protein family/group databases

MEROPSiI31.953.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like growth factor-binding protein 2-A
Short name:
IGF-binding protein 2-A
Short name:
IGFBP-2-A
Short name:
IGFBP-2a
Gene namesi
Name:igfbp2a
Synonyms:igfbp2, igfbp2b
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437 Componenti: Chromosome 6

Organism-specific databases

ZFINiZDB-GENE-000125-12. igfbp2a.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
  2. extracellular space Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 276254Insulin-like growth factor-binding protein 2-APRO_0000014376Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi180 ↔ 214PROSITE-ProRule annotation
Disulfide bondi225 ↔ 236PROSITE-ProRule annotation
Disulfide bondi238 ↔ 259PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

In embryos at 24 hpf, initially expressed in the lens and cranial region, and at 48 and 72 hpf in the brain boundary vasculature. Expression in these regions persists throughout the hatching period and by 96 hpf expression is most abundant in the liver. In both male and female adults, highest expression is in the liver with modest expression in the brain. In male but not females adults, expressed at a low level in muscle and gonad. Also expressed in the adult intestine.2 Publications

Developmental stagei

Not expressed until 10 hpf. Expression gradually increases from 10 to 36 hpf and is maintained at high levels thereafter.1 Publication

Inductioni

Expression increases in response to prolonged fasting but decreases in response to growth hormone (GH).1 Publication

Gene expression databases

BgeeiQ9PTH3.

Interactioni

Subunit structurei

Interacts equally well with igf1 and igf2.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9PTH3.
SMRiQ9PTH3. Positions 26-103, 172-267.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 10582IGFBP N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini177 – 25983Thyroglobulin type-1PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi254 – 2563Cell attachment siteSequence Analysis

Sequence similaritiesi

Contains 1 IGFBP N-terminal domain.PROSITE-ProRule annotation
Contains 1 thyroglobulin type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG47500.
GeneTreeiENSGT00550000074457.
HOGENOMiHOG000253012.
InParanoidiQ9PTH3.
OMAiGAGTCEK.
OrthoDBiEOG74N5HG.
PhylomeDBiQ9PTH3.
TreeFamiTF331211.

Family and domain databases

Gene3Di4.10.40.20. 1 hit.
4.10.800.10. 1 hit.
InterProiIPR009030. Growth_fac_rcpt_N_dom.
IPR012210. IGFBP-2.
IPR000867. IGFBP-like.
IPR009168. IGFBP1-6.
IPR022321. IGFBP_1-6_chordata.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERiPTHR11551. PTHR11551. 1 hit.
PTHR11551:SF5. PTHR11551:SF5. 1 hit.
PfamiPF00219. IGFBP. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PRINTSiPR01976. IGFBPFAMILY.
PR01978. IGFBPFAMILY2.
SMARTiSM00121. IB. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9PTH3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSYVSCGLL LALVTFHGTA RSEMVFRCPS CTAERQAACP MLTETCGEIV
60 70 80 90 100
REPGCGCCPV CARQEGEQCG VYTPRCSSGL RCYPKPDSEL PLELLVQGLG
110 120 130 140 150
RCGRKVDTEP TGSAEPREVS GEVQDPLDIG LTEVPPIRKP TKDSPWKESA
160 170 180 190 200
VLQHRQQLKS KMKYHKVEDP KAPHAKQSQC QQELDQVLER ISKITFKDNR
210 220 230 240 250
TPLEDLYSLH IPNCDKRGQY NLKQCKMSVN GYRGECWCVN PHTGRPMPTS
260 270
PLIRGDPNCN QYLDGQEMDP SVDPPN
Length:276
Mass (Da):30,764
Last modified:May 1, 2000 - v1
Checksum:i37AB561B7C9947D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF198033 mRNA. Translation: AAF23123.1.
BC056331 mRNA. Translation: AAH56331.1.
BC153606 mRNA. Translation: AAI53607.1.
RefSeqiNP_571533.1. NM_131458.2.
UniGeneiDr.78120.

Genome annotation databases

EnsembliENSDART00000074327; ENSDARP00000068816; ENSDARG00000052470.
GeneIDi794176.
KEGGidre:794176.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF198033 mRNA. Translation: AAF23123.1.
BC056331 mRNA. Translation: AAH56331.1.
BC153606 mRNA. Translation: AAI53607.1.
RefSeqiNP_571533.1. NM_131458.2.
UniGeneiDr.78120.

3D structure databases

ProteinModelPortaliQ9PTH3.
SMRiQ9PTH3. Positions 26-103, 172-267.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiI31.953.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000074327; ENSDARP00000068816; ENSDARG00000052470.
GeneIDi794176.
KEGGidre:794176.

Organism-specific databases

CTDi794176.
ZFINiZDB-GENE-000125-12. igfbp2a.

Phylogenomic databases

eggNOGiNOG47500.
GeneTreeiENSGT00550000074457.
HOGENOMiHOG000253012.
InParanoidiQ9PTH3.
OMAiGAGTCEK.
OrthoDBiEOG74N5HG.
PhylomeDBiQ9PTH3.
TreeFamiTF331211.

Enzyme and pathway databases

ReactomeiREACT_289688. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Miscellaneous databases

NextBioi20931744.

Gene expression databases

BgeeiQ9PTH3.

Family and domain databases

Gene3Di4.10.40.20. 1 hit.
4.10.800.10. 1 hit.
InterProiIPR009030. Growth_fac_rcpt_N_dom.
IPR012210. IGFBP-2.
IPR000867. IGFBP-like.
IPR009168. IGFBP1-6.
IPR022321. IGFBP_1-6_chordata.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERiPTHR11551. PTHR11551. 1 hit.
PTHR11551:SF5. PTHR11551:SF5. 1 hit.
PfamiPF00219. IGFBP. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PRINTSiPR01976. IGFBPFAMILY.
PR01978. IGFBPFAMILY2.
SMARTiSM00121. IB. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Insulin-like growth factor binding protein 2 is a growth inhibitory protein conserved in zebrafish."
    Duan C., Ding J., Li Q., Tsai W., Pozios K.
    Proc. Natl. Acad. Sci. U.S.A. 96:15274-15279(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH IGF1 AND IGF2, TISSUE SPECIFICITY, INDUCTION.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.
  3. "Duplication of the IGFBP-2 gene in teleost fish: protein structure and functionality conservation and gene expression divergence."
    Zhou J., Li W., Kamei H., Duan C.
    PLoS ONE 3:E3926-E3926(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiIBP2A_DANRE
AccessioniPrimary (citable) accession number: Q9PTH3
Secondary accession number(s): A8E5I5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 1, 2000
Last modified: April 1, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.