Reviewed, UniProtKB/Swiss-Prot Q9PTE6 (TPST1_DANRE)

Names and origin

Protein names

Recommended name:
    Protein-tyrosine sulfotransferase 1
    EC=2.8.2.20
Alternative name(s):
    Tyrosylprotein sulfotransferase-1
      Short name=TPST-1

Gene names

Name:

tpst1

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides By similarity.
Catalytic activity	3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the protein sulfotransferase family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-tyrosine sulfotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Protein-tyrosine sulfotransferase 1		PRO_0000189828
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 25	17	Signal-anchor for type II membrane protein Potential
	Topological domain	26 – 355	330	Lumenal Potential
Amino acid modifications
	Glycosylation	55	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	211	1	K → R in AAF18448. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9PTE6-1 [UniParc]. Last modified February 1, 2005. Version 2. Checksum: 02F45B99C9C7E502	FASTA	355	40,198
	10 20 30 40 50 60 MIGKLKQNLL VACLVISSVT VFYLCRHAMD CHHRIEERSQ PLLSSLHATL RTGQNLSTPF 70 80 90 100 110 120 IYNKDMPLIF IGGVPRSGTT LMRAMLDAHP DVRCGEETRV IPRILAMKQM WSRSGREKMR 130 140 150 160 170 180 LDEAGVTDEV LDSAMQAFLL EIIVKHGEPA NYLCNKDPFA LKSLTYLAKI FPHAKFILMV 190 200 210 220 230 240 RDGRASVHSM ISRKVTIAGF DLSSYRDCLT KWNRAIETMY TQCLEAADKC LPVHYEQLVL 250 260 270 280 290 300 HPEKWMRTLL RFLNIPWNDA VLHHEELIGK AGGVSLSKVE RSTDQVIKPV NVEALSKWVG 310 320 330 340 350 KIPLDVQRDM AVIAPMLARL GYDPHANPPN YGRPDPLVLD NTRRLQKSPE KPNPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tyrosylprotein sulfotransferase-1 from Danio rerio." Moore K.L., Dudley A.A. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: AB.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: AB.
[3]	"Isolation of zebrafish tyrosylprotein sulfotransferase 1 cDNA." Kim C.H. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-256.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF510736 mRNA. Translation: AAO42986.1. BC056786 mRNA. Translation: AAH56786.1. AF204241 mRNA. Translation: AAF18448.1.
IPI	IPI00512152.
RefSeq	NP_571478.1.
UniGene	Dr.118149
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9PTE6.
Genome annotation databases
Ensembl	ENSDART00000114464; ENSDARP00000101352; ENSDARG00000073872; Danio rerio. [Genome view]
GeneID	30677.
KEGG	dre:30677.
Organism-specific databases
CTD	30677.
ZFIN	ZDB-GENE-000210-10. tpst1.
Phylogenomic databases
HOVERGEN	Q9PTE6.
Enzyme and pathway databases
BRENDA	2.8.2.20. 96826.
Gene expression databases
Bgee	Q9PTE6.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

TPST1_DANRE

Accession

Primary (citable) accession number: Q9PTE6
Secondary accession number(s): Q804S9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	February 1, 2005
Last modified:	October 13, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project