Reviewed, UniProtKB/Swiss-Prot Q9PS28 (VSP34_CERCE)

Names and origin

Protein names	Recommended name:     Proteinase RP34     EC=3.4.21.74
Organism	Cerastes cerastes (Horned desert viper)
Taxonomic identifier	8697 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Viperidae › Viperinae › Cerastes

Protein attributes

Sequence length	33 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Thrombin-like snake venom serine protease. Displays proteolytic activity toward the natural substrate casein as well as clotting activity on fibrinogen. Shows both arginine-ester hydrolase and amidase activities on synthetic substrates. Ref.1
Catalytic activity	Selective cleavage of Arg-|-Xaa bond in fibrinogen, to form fibrin, and release fibrinopeptide A. The specificity of further degradation of fibrinogen varies with species origin of the enzyme.
Subunit structure	Homodimer. Ref.1
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the peptidase S1 family. Snake venom subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Blood coagulation
Cellular component	Secreted
Molecular function	Hydrolase Protease Serine protease Toxin
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›33	›33	Proteinase RP34		PRO_0000294991
Regions
	Domain	1 – ›33	›33	Peptidase S1
Experimental info
	Non-terminal residue	33	1

Sequences

	Sequence		Length	Mass (Da)
	Q9PS28-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 123F8467054D32E3	FASTA	33	3,786
	10 20 30 VIGGDEXDIN EHRSLALMYX SWSHRFIXXG XLI

References

[1]

"A fibrinogen-clotting serine proteinase from Cerastes cerastes (horned viper) venom with arginine-esterase and amidase activities. Purification, characterization and kinetic parameter determination." Laraba-Djebari F., Martin-Eauclaire M.-F., Marchot P. Toxicon 30:1399-1410(1992) [PubMed: 1485336] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, SUBUNIT. Tissue: Venom.

Cross-references

Sequence databases
PIR	A44181.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q9PS28.
Enzyme and pathway databases
BRENDA	3.4.21.74. 18553.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. Partial match. PS00134. TRYPSIN_HIS. Partial match. PS00135. TRYPSIN_SER. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VSP34_CERCE

Accession

Primary (citable) accession number: Q9PS28

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Tox-Prot (Toxin Annotation Project)