Reviewed, UniProtKB/Swiss-Prot Q9PRP4 (VSPA_LACMR)

Names and origin

Protein names	Recommended name:     Gyroxin analog     EC=3.4.21.74 Alternative name(s):     LMR-47     Thrombin-like enzyme
Organism	Lachesis muta rhombeata (Bushmaster)
Taxonomic identifier	60219 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Viperidae › Crotalinae › Lachesis

Protein attributes

Sequence length	30 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Thrombin-like snake venom serine protease. Exhibits strong N-p-tosyl-L-arginine methyl esterase activity, hydrolyzes tripeptide nitroanilide derivatives weakly or not at all, and specifically hydrolyzes the alpha chain of fibrinogen.
Catalytic activity	Selective cleavage of Arg-|-Xaa bond in fibrinogen, to form fibrin, and release fibrinopeptide A. The specificity of further degradation of fibrinogen varies with species origin of the enzyme.
Enzyme regulation	Inactivated by antipain, PMSF, TPCK, leupeptin, pepstatin, EDTA, and E-64 (trans-epoxysuccinylleucylamido(4-guanidino)butane).
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Post-translational modification	Glycosylated. Ref.1
Sequence similarities	Belongs to the peptidase S1 family. Snake venom subfamily. Contains 1 peptidase S1 domain.
biophysicochemical properties	Kinetic parameters: KM=12 µM for N-p-tosyl-L-arginine methyl ester (TAME) KM=589 µM for NBz-Pro-Phe-Arg-pNA KM=1320 µM for NBz-Val-Gly-Arg-pNA KM=857 µM for NBz-DL-Arg-pNA Vmax=877.0 nmol/sec/mg enzyme for N-p-tosyl-L-arginine methyl ester (TAME) Vmax=51.0 nmol/sec/mg enzyme for NBz-Pro-Phe-Arg-pNA Vmax=75.0 nmol/sec/mg enzyme for NBz-Val-Gly-Arg-pNA Vmax=39.3 nmol/sec/mg enzyme for NBz-DL-Arg-pNA

Ontologies

Keywords
Biological process	Blood coagulation
Cellular component	Secreted
Molecular function	Hydrolase Protease Serine protease Toxin
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›30	›30	Gyroxin analog		PRO_0000295178
Regions
	Domain	1 – ›30	›30	Peptidase S1
Experimental info
	Non-terminal residue	30	1

Sequences

	Sequence		Length	Mass (Da)
	Q9PRP4-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: FA1B2609921853DB	FASTA	30	3,171
	10 20 30 VIGGDECNIN EHRFLVALYD GLSGTFLCGG

References

[1]

"Purification and partial characterization of a thrombin-like/gyroxin enzyme from bushmaster (Lachesis muta rhombeata) venom." Aguiar A.S., Alves C.R., Melgarejo A., Giovanni-de-Simone S. Toxicon 34:555-565(1996) [PubMed: 8783450] [Abstract] Cited for: PROTEIN SEQUENCE, BIOPHYSICOCHEMICAL PROPERTIES, GLYCOSYLATION. Tissue: Venom.

Cross-references

3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q9PRP4.
Enzyme and pathway databases
BRENDA	3.4.21.74. 296551.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. Partial match. PS00134. TRYPSIN_HIS. Partial match. PS00135. TRYPSIN_SER. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VSPA_LACMR

Accession

Primary (citable) accession number: Q9PRP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Tox-Prot (Toxin Annotation Project)