Reviewed, UniProtKB/Swiss-Prot Q9PR63 (GYRA_UREPA)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit A
    EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	UU082

Organism

Ureaplasma parvum (Ureaplasma urealyticum biotype 1) [Complete proteome] [HAMAP]

Taxonomic identifier

134821 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Ureaplasma

Protein attributes

Sequence length	840 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 840	840	DNA gyrase subunit A		PRO_0000145272
Sites
	Active site	139	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PR63-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 8D3DC66EB4DC1CF3	FASTA	840	94,252
	10 20 30 40 50 60 MALKKPKKSR LTTEEIKQQL EGSTIKEQSI TKEVETSFLD YSMSVIVARA LPDVRDGFKP 70 80 90 100 110 120 VHRRALFAAF ENGMTHDKPY KKSARWVGDV IGKYHPHGDQ AVYQTIVRMA QDFSMRYLLV 130 140 150 160 170 180 DGHGNFGSID GDSAAAMRYT EARLSKISYE LLKYIDKETV DFVPNYDASE QEPSVLPSGF 190 200 210 220 230 240 PNLLTNGTTG IAVGMATNIP PHNLTEVCQA IKAYAKNHNV TISEIMEYLK GPDFPTGAEI 250 260 270 280 290 300 YGDSGIIKYF NTGRGSVTIR SKYEIEDIGQ GRVAIVVTEI PYMVNKANLI EKIVELVTNK 310 320 330 340 350 360 QIEGISDLRD ESSRDGIRIV IEVKRDVIPE VLLNKLFKTT PLQTNFSVNN LALVNGVPMV 370 380 390 400 410 420 LNIKEMIKYY FEHQIEILVR RTNFDLKKAK ERIHIVEGLV IAVNNIDEVI KIIKASGDDD 430 440 450 460 470 480 IASKSLIQRF DLTELQTKAI LEMRLRALTG LNIDKLKKEY EDLILVIKDL EDVLNNYNRQ 490 500 510 520 530 540 VNIICENLDY LIEKFGDERR TEIMYGVSSH IDDEDLIPVE DIVVTMSKRG YFKRLPIDTY 550 560 570 580 590 600 KNQRRGGVGV QGLKTYEDDD VEKILVANTH TDLLFFSDLG RVYRLRGHEV PLGSRQSKGI 610 620 630 640 650 660 PAINFLPIEK SESILTILPI DNYDQGSLFF TTSKGIIKRA NLSDFESIRA NGKIAITLKD 670 680 690 700 710 720 GDKLFSVMQT LGNDEVFIGA SNGNVIRFNE NDAREMGRIA TGVKGINLEN DEYVVGTGLS 730 740 750 760 770 780 SHGEYVLAVG SKGLGKLTDI NDYRLTKRGA KGVNTLKVND KTGNLVSIKV VNREEEALII 790 800 810 820 830 840 TTSGKVIRLS IKDISVIGRN TSGVKLISLE NKEEVKSIAI FKKEEINDEQ LLQENDYNLK

References

[1]	"The complete sequence of the mucosal pathogen Ureaplasma urealyticum." Glass J.I., Lefkowitz E.J., Glass J.S., Heiner C.R., Chen E.Y., Cassell G.H. Nature 407:757-762(2000) [PubMed: 11048724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700970 / Serovar 3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF222894 Genomic DNA. Translation: AAF30487.1.
RefSeq	NP_077912.1.
3D structure databases
HSSP	HSSP built from PDB template 1AB4 based on UniProtKB P09097.
ModBase	Search...
Genome annotation databases
GeneID	876159.
GenomeReviews	Gene locus UU082 in contig AF222894_GR.
KEGG	uur:UU082.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9PR63.
OMA	ELGNDWN.
Enzyme and pathway databases
BioCyc	UURE95667:UU082-MON.
BRENDA	5.99.1.3. 281353.
Family and domain databases
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
ProDom	PD000742. DNA_topoisoIV. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_UREPA

Accession

Primary (citable) accession number: Q9PR63

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)