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Q9PQ79

- SYI_UREPA

UniProt

Q9PQ79 - SYI_UREPA

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Ureaplasma parvum serovar 3 (strain ATCC 700970)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
Binding sitei596 – 5961ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciUPAR273119:GHVP-428-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:UU410
OrganismiUreaplasma parvum serovar 3 (strain ATCC 700970)
Taxonomic identifieri273119 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeUreaplasma
ProteomesiUP000000423: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 900900Isoleucine--tRNA ligasePRO_0000098496Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi273119.UU410.

Structurei

3D structure databases

ProteinModelPortaliQ9PQ79.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi593 – 5975"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiRYVEGKW.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PQ79-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKDYKSTLNM PTTGFEMRAN LNVKEPKIQQ FWVEHQIYEK LLTKNKDKKP
60 70 80 90 100
FILHDGPPYA NGNIHIGHAL NKILKDFVVS YHNMNNYYSP YIPGWDTHGL
110 120 130 140 150
PIEVALSKKI KLSNLSVNER REQCKKYALE QVNNQIQQFL RLGMISDFKQ
160 170 180 190 200
RYLTLDHNYE IDQLKLFTYM LKKGFIYQDF KPVFWSWSSQ TALAESEIEY
210 220 230 240 250
ADRQSSAIYV KMKVVDHNDL FTDKPTSLVI WTTTPWTLPA NLAIAIHPEL
260 270 280 290 300
VYSLIEYKNE NYIIAKPLVE TFVKKVGFED YKWIKDFKAN TLEKIKYISP
310 320 330 340 350
ISKKHAFVIM DEYVSANDGT GLVHNAPAFG LEDYYACKKY GIETVVMIDQ
360 370 380 390 400
FGKYNALVND LELENMFYED ANQVILNRLM NEHLLIHHEV ITHSVAHDWR
410 420 430 440 450
TKKPVMYRAT KQWFVSIEKI LPNILQTLNN DVKSTSFRGI ERMHEMIVNR
460 470 480 490 500
KEWCISRQRV WGVPIPMIFD ENHNAIMDHE LVENIINVLN EKGVNAWFDL
510 520 530 540 550
DVNAFLTPKY LSMKNKTFYK EKDIMDVWFD SGSSYNILGH YNLNYPADVY
560 570 580 590 600
LEGYDQYRGW FNSSLITGTI LNNRAPYKYL VAHGMVLDGE GYKMSKSKGN
610 620 630 640 650
VVDPLDVCKI YGADVLRLWI ANSDYQNDTR ISEEILKQNA EIYRRIRNTL
660 670 680 690 700
FKYSLSILND FEPSVDFSFN VRQEEQFVLN EFNELHIKVI KAYENFDYQT
710 720 730 740 750
VVKLFNKFIL DLSSWYFENI KDDMYCLAIN DPIRKQIQSA VYWILKNSLI
760 770 780 790 800
DLTPIIPHTT EEAYSFLKDA NKKESIRLED FYDQSQFQFK KGIAHVKAFF
810 820 830 840 850
SIKDQIFNEL ENARKNNILK KNNEAFVTIA KNLILDDYLI NNPKLLAKWF
860 870 880 890 900
GVAKIEFANN TNVANANFKK CLRCWNHFPD EEMYNDELSM NCYKVINKIK
Length:900
Mass (Da):105,806
Last modified:October 1, 2000 - v1
Checksum:iFA478902AE347D87
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF222894 Genomic DNA. Translation: AAF30821.1.
RefSeqiNP_078246.1. NC_002162.1.

Genome annotation databases

EnsemblBacteriaiAAF30821; AAF30821; UU410.
GeneIDi876236.
KEGGiuur:UU410.
PATRICi20534400. VBIUrePar45146_0426.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF222894 Genomic DNA. Translation: AAF30821.1 .
RefSeqi NP_078246.1. NC_002162.1.

3D structure databases

ProteinModelPortali Q9PQ79.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273119.UU410.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAF30821 ; AAF30821 ; UU410 .
GeneIDi 876236.
KEGGi uur:UU410.
PATRICi 20534400. VBIUrePar45146_0426.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi RYVEGKW.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci UPAR273119:GHVP-428-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete sequence of the mucosal pathogen Ureaplasma urealyticum."
    Glass J.I., Lefkowitz E.J., Glass J.S., Heiner C.R., Chen E.Y., Cassell G.H.
    Nature 407:757-762(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700970.

Entry informationi

Entry nameiSYI_UREPA
AccessioniPrimary (citable) accession number: Q9PQ79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3