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Q9PQ79

- SYI_UREPA

UniProt

Q9PQ79 - SYI_UREPA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Ureaplasma parvum serovar 3 (strain ATCC 700970)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
    Binding sitei596 – 5961ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciUPAR273119:GHVP-428-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:UU410
    OrganismiUreaplasma parvum serovar 3 (strain ATCC 700970)
    Taxonomic identifieri273119 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeUreaplasma
    ProteomesiUP000000423: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 900900Isoleucine--tRNA ligasePRO_0000098496Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi273119.UU410.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9PQ79.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi593 – 5975"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiRYVEGKW.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9PQ79-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKDYKSTLNM PTTGFEMRAN LNVKEPKIQQ FWVEHQIYEK LLTKNKDKKP    50
    FILHDGPPYA NGNIHIGHAL NKILKDFVVS YHNMNNYYSP YIPGWDTHGL 100
    PIEVALSKKI KLSNLSVNER REQCKKYALE QVNNQIQQFL RLGMISDFKQ 150
    RYLTLDHNYE IDQLKLFTYM LKKGFIYQDF KPVFWSWSSQ TALAESEIEY 200
    ADRQSSAIYV KMKVVDHNDL FTDKPTSLVI WTTTPWTLPA NLAIAIHPEL 250
    VYSLIEYKNE NYIIAKPLVE TFVKKVGFED YKWIKDFKAN TLEKIKYISP 300
    ISKKHAFVIM DEYVSANDGT GLVHNAPAFG LEDYYACKKY GIETVVMIDQ 350
    FGKYNALVND LELENMFYED ANQVILNRLM NEHLLIHHEV ITHSVAHDWR 400
    TKKPVMYRAT KQWFVSIEKI LPNILQTLNN DVKSTSFRGI ERMHEMIVNR 450
    KEWCISRQRV WGVPIPMIFD ENHNAIMDHE LVENIINVLN EKGVNAWFDL 500
    DVNAFLTPKY LSMKNKTFYK EKDIMDVWFD SGSSYNILGH YNLNYPADVY 550
    LEGYDQYRGW FNSSLITGTI LNNRAPYKYL VAHGMVLDGE GYKMSKSKGN 600
    VVDPLDVCKI YGADVLRLWI ANSDYQNDTR ISEEILKQNA EIYRRIRNTL 650
    FKYSLSILND FEPSVDFSFN VRQEEQFVLN EFNELHIKVI KAYENFDYQT 700
    VVKLFNKFIL DLSSWYFENI KDDMYCLAIN DPIRKQIQSA VYWILKNSLI 750
    DLTPIIPHTT EEAYSFLKDA NKKESIRLED FYDQSQFQFK KGIAHVKAFF 800
    SIKDQIFNEL ENARKNNILK KNNEAFVTIA KNLILDDYLI NNPKLLAKWF 850
    GVAKIEFANN TNVANANFKK CLRCWNHFPD EEMYNDELSM NCYKVINKIK 900
    Length:900
    Mass (Da):105,806
    Last modified:October 1, 2000 - v1
    Checksum:iFA478902AE347D87
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF222894 Genomic DNA. Translation: AAF30821.1.
    RefSeqiNP_078246.1. NC_002162.1.

    Genome annotation databases

    EnsemblBacteriaiAAF30821; AAF30821; UU410.
    GeneIDi876236.
    KEGGiuur:UU410.
    PATRICi20534400. VBIUrePar45146_0426.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF222894 Genomic DNA. Translation: AAF30821.1 .
    RefSeqi NP_078246.1. NC_002162.1.

    3D structure databases

    ProteinModelPortali Q9PQ79.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273119.UU410.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAF30821 ; AAF30821 ; UU410 .
    GeneIDi 876236.
    KEGGi uur:UU410.
    PATRICi 20534400. VBIUrePar45146_0426.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi RYVEGKW.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci UPAR273119:GHVP-428-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete sequence of the mucosal pathogen Ureaplasma urealyticum."
      Glass J.I., Lefkowitz E.J., Glass J.S., Heiner C.R., Chen E.Y., Cassell G.H.
      Nature 407:757-762(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700970.

    Entry informationi

    Entry nameiSYI_UREPA
    AccessioniPrimary (citable) accession number: Q9PQ79
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3