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Q9PQ79

- SYI_UREPA

UniProt

Q9PQ79 - SYI_UREPA

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Protein

Isoleucine--tRNA ligase

Gene
ileS, UU410
Organism
Ureaplasma parvum serovar 3 (strain ATCC 700970)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylate By similarity
Binding sitei596 – 5961ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciUPAR273119:GHVP-428-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:UU410
OrganismiUreaplasma parvum serovar 3 (strain ATCC 700970)
Taxonomic identifieri273119 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeUreaplasma
ProteomesiUP000000423: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 900900Isoleucine--tRNA ligaseUniRule annotationPRO_0000098496Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi273119.UU410.

Structurei

3D structure databases

ProteinModelPortaliQ9PQ79.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi593 – 5975"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiRYVEGKW.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PQ79-1 [UniParc]FASTAAdd to Basket

« Hide

MKDYKSTLNM PTTGFEMRAN LNVKEPKIQQ FWVEHQIYEK LLTKNKDKKP    50
FILHDGPPYA NGNIHIGHAL NKILKDFVVS YHNMNNYYSP YIPGWDTHGL 100
PIEVALSKKI KLSNLSVNER REQCKKYALE QVNNQIQQFL RLGMISDFKQ 150
RYLTLDHNYE IDQLKLFTYM LKKGFIYQDF KPVFWSWSSQ TALAESEIEY 200
ADRQSSAIYV KMKVVDHNDL FTDKPTSLVI WTTTPWTLPA NLAIAIHPEL 250
VYSLIEYKNE NYIIAKPLVE TFVKKVGFED YKWIKDFKAN TLEKIKYISP 300
ISKKHAFVIM DEYVSANDGT GLVHNAPAFG LEDYYACKKY GIETVVMIDQ 350
FGKYNALVND LELENMFYED ANQVILNRLM NEHLLIHHEV ITHSVAHDWR 400
TKKPVMYRAT KQWFVSIEKI LPNILQTLNN DVKSTSFRGI ERMHEMIVNR 450
KEWCISRQRV WGVPIPMIFD ENHNAIMDHE LVENIINVLN EKGVNAWFDL 500
DVNAFLTPKY LSMKNKTFYK EKDIMDVWFD SGSSYNILGH YNLNYPADVY 550
LEGYDQYRGW FNSSLITGTI LNNRAPYKYL VAHGMVLDGE GYKMSKSKGN 600
VVDPLDVCKI YGADVLRLWI ANSDYQNDTR ISEEILKQNA EIYRRIRNTL 650
FKYSLSILND FEPSVDFSFN VRQEEQFVLN EFNELHIKVI KAYENFDYQT 700
VVKLFNKFIL DLSSWYFENI KDDMYCLAIN DPIRKQIQSA VYWILKNSLI 750
DLTPIIPHTT EEAYSFLKDA NKKESIRLED FYDQSQFQFK KGIAHVKAFF 800
SIKDQIFNEL ENARKNNILK KNNEAFVTIA KNLILDDYLI NNPKLLAKWF 850
GVAKIEFANN TNVANANFKK CLRCWNHFPD EEMYNDELSM NCYKVINKIK 900
Length:900
Mass (Da):105,806
Last modified:October 1, 2000 - v1
Checksum:iFA478902AE347D87
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF222894 Genomic DNA. Translation: AAF30821.1.
RefSeqiNP_078246.1. NC_002162.1.

Genome annotation databases

EnsemblBacteriaiAAF30821; AAF30821; UU410.
GeneIDi876236.
KEGGiuur:UU410.
PATRICi20534400. VBIUrePar45146_0426.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF222894 Genomic DNA. Translation: AAF30821.1 .
RefSeqi NP_078246.1. NC_002162.1.

3D structure databases

ProteinModelPortali Q9PQ79.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273119.UU410.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAF30821 ; AAF30821 ; UU410 .
GeneIDi 876236.
KEGGi uur:UU410.
PATRICi 20534400. VBIUrePar45146_0426.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi RYVEGKW.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci UPAR273119:GHVP-428-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete sequence of the mucosal pathogen Ureaplasma urealyticum."
    Glass J.I., Lefkowitz E.J., Glass J.S., Heiner C.R., Chen E.Y., Cassell G.H.
    Nature 407:757-762(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700970.

Entry informationi

Entry nameiSYI_UREPA
AccessioniPrimary (citable) accession number: Q9PQ79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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