Q9PQ33 (SYFB_UREPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase beta chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase beta chain
Short name=PheRS

Gene names

Name:	pheT
Ordered Locus Names:	UU457

Organism

Ureaplasma parvum serovar 3 (strain ATCC 700970) [Complete proteome] [HAMAP]

Taxonomic identifier

273119 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Ureaplasma

Protein attributes

Sequence length	772 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00283
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00283
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00283.
Sequence similarities	Belongs to the phenylalanyl-tRNA synthetase beta chain family. Type 1 subfamily. Contains 1 B5 domain. Contains 1 FDX-ACB domain. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 772	772	Phenylalanine--tRNA ligase beta chain HAMAP MF_00283		PRO_0000126980
Regions
	Domain	40 – 158	119	tRNA-binding
	Domain	397 – 468	72	B5
	Domain	691 – 772	82	FDX-ACB
Sites
	Metal binding	446	1	Magnesium By similarity
	Metal binding	452	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	455	1	Magnesium By similarity
	Metal binding	456	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PQ33 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: CCBE070438AEB6E6	FASTA	772	88,192
	10 20 30 40 50 60 MILSLNLLHK ISPKLKKIPL NELCAALMDL GCEVETINSI KPSTNLVFAK VLEKTRHPNA 70 80 90 100 110 120 NHLNLVKVKA NQKVYEIVCG ANNFSVHNWV VLAKLNAELA NGLKITPREL RGYVSNGMLC 130 140 150 160 170 180 AYSEINPQAT SFLTSTDLDG ILVLDDNYDH YKTPNQIFNL DDVILDLSIP SNRNDLNGYF 190 200 210 220 230 240 WIAKELCAYF DFEYVVDATI NHRPHKEIID VRILSDDVNS YGIIEVKNIQ NYILKWNTKS 250 260 270 280 290 300 ILINNQIKVL NNFADNMNFL TLLTANPLHA FDARKISGQI VVKNAEEDAI LLGLDQKEYL 310 320 330 340 350 360 IKKGDLIIVD DQKILALAGI IGSNDSKIDA TSTTAYIECA NFNPMLIANT ARRLKINTAA 370 380 390 400 410 420 AMRFSKPLTN YVTKVTLKKL LANFKSDAKL IYYFKHSVHN VIKNKINQVS DFVGAEIDLD 430 440 450 460 470 480 TAQTFLKRLG YKINKSNLIT PSHRYDVLNE FDVYEDIMKK ISIQEIKPQP ISFDILNFEN 490 500 510 520 530 540 NLAYDFEKKV SDFLVDQGLF ECKTYNLKNQ TQAHEFNFFN FKQAYEINNP TSNMRSHLKL 550 560 570 580 590 600 NNLNSLLEVL EYNQNQKNEL ENIFEISKIN PVDSSQQTVL SIILCKPLIN SKINDSLIVN 610 620 630 640 650 660 NFVTTKALLH ALLTKLNIDY AYDKNHVVNE LYDNNQLALI NANKQVFGFI GQLKNQVKKT 670 680 690 700 710 720 YGLSNDIFII NLNLTSYLNQ KQVITKVIKP SMYHDVIRDI SVKLASDVDL NNIIANIKKI 730 740 750 760 770 KNIRKVEISD LYIKDDGIIY TFKYYINDHL SNLSSEQIII IEQEVNNYLK QF

References

[1]	"The complete sequence of the mucosal pathogen Ureaplasma urealyticum." Glass J.I., Lefkowitz E.J., Glass J.S., Heiner C.R., Chen E.Y., Cassell G.H. Nature 407:757-762(2000) [PubMed: 11048724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700970.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF222894 Genomic DNA. Translation: AAF30869.1.
RefSeq	NP_078294.1. NC_002162.1.
3D structure databases
ProteinModelPortal	Q9PQ33.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	876345.
GenomeReviews	Gene locus UU457 in contig AF222894_GR.
KEGG	uur:UU457.
PATRIC	20534497. VBIUrePar45146_0473.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734515.
OMA	ELERKCK.
ProtClustDB	PRK00629.
Enzyme and pathway databases
BioCyc	UURE95667:UU457-MONOMER.
Family and domain databases
HAMAP	MF_00283. Phe_tRNA_synth_beta1. [Tree]
InterPro	IPR005146. B3/B4_tRNA-bd. IPR009061. DNA-bd_dom_put. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004532. Phe-tRNA-synth_IIc_bsu_bac. IPR020825. Phe-tRNA_synthase_B3/B4. IPR005121. PheS_beta_Fdx_antiC-bd. IPR002547. tRNA-bd_dom. IPR005147. tRNA_synthase_B5-dom. [Graphical view]
Gene3D	G3DSA:3.50.40.10. B3_4. 1 hit. G3DSA:3.30.56.20. B5. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01890.
Pfam	PF03483. B3_4. 1 hit. PF03484. B5. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
SMART	SM00873. B3_4. 1 hit. SM00874. B5. 1 hit. [Graphical view]
SUPFAM	SSF56037. B3_4. 1 hit. SSF50249. Nucleic_acid_OB. 1 hit. SSF46955. Putativ_DNA_bind. 1 hit.
TIGRFAMs	TIGR00472. PheT_bact. 1 hit.
PROSITE	PS51483. B5. 1 hit. PS51447. FDX_ACB. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFB_UREPA

Accession

Primary (citable) accession number: Q9PQ33

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program