Q9PPP0 (SYE_UREPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	UU599

Organism

Ureaplasma parvum serovar 3 (strain ATCC 700970) [Complete proteome] [HAMAP]

Taxonomic identifier

273119 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Ureaplasma

Protein attributes

Sequence length	482 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 482	482	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000119690
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022_B
	Motif	252 – 256	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	255	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PPP0 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 3977C1BCFA31C16C	FASTA	482	55,909
	10 20 30 40 50 60 MKIRTRYAPS PTGYLHIGGA RTALFNYLLA KAYGGDFIIR IEDTDIERNV EGGINSQLDF 70 80 90 100 110 120 LAWMGIIPDE SIRNPKAFGP YIQSEKLKHY EKLALDLVDQ KKAYFCFCSK EQLDADRELA 130 140 150 160 170 180 EKSHQTPKYK RHCLNLDKKT IESNLLQNKE YTIRLKINEN MEYSWDDLIR GKISIPGSAL 190 200 210 220 230 240 TDPVILKSNK IAMYNFAVVI DDYEMQISHV IRGEEHISNT PYQLAIAQAL NYDITKIKYG 250 260 270 280 290 300 HLSIIVDETG KKLSKRNLSL KQFVSDYEKD GYWPHAITNF VALLGWSPKN NDEIMSLETM 310 320 330 340 350 360 IKNFDINNLS KSPAFFDINK MNWFSTQYFN NITQEEFINF IKKHSLTKEL VLNDYTFINK 370 380 390 400 410 420 CLLFKSHIIN LKQLIDLVIE QFNCDKKVLA SDVDYIKKNQ LITVVRVFYE QLIINDEFNE 430 440 450 460 470 480 EFIKEIIKKV QIITNNKGAN LYMPIRIATT FSSHGPELAK TICYLGREKV LKNLINILKI LD

References

[1]	"The complete sequence of the mucosal pathogen Ureaplasma urealyticum." Glass J.I., Lefkowitz E.J., Glass J.S., Heiner C.R., Chen E.Y., Cassell G.H. Nature 407:757-762(2000) [PubMed: 11048724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700970.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF222894 Genomic DNA. Translation: AAF31013.1.
RefSeq	NP_078438.1. NC_002162.1.
3D structure databases
ProteinModelPortal	Q9PPP0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	876116.
GenomeReviews	Gene locus UU599 in contig AF222894_GR.
KEGG	uur:UU599.
PATRIC	20534835. VBIUrePar45146_0622.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	DDFDMEI.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	UURE95667:UU599-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_UREPA

Accession

Primary (citable) accession number: Q9PPP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program