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Q9PPC2 (DDL_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Synonyms:ddlA
Ordered Locus Names:Cj0798c
OrganismCampylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168) [Reference proteome] [HAMAP]
Taxonomic identifier192222 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 346346D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177800

Regions

Domain133 – 327195ATP-grasp
Nucleotide binding159 – 21153ATP By similarity

Sites

Metal binding2841Magnesium or manganese 1 By similarity
Metal binding2961Magnesium or manganese 1 By similarity
Metal binding2961Magnesium or manganese 2 By similarity
Metal binding2981Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9PPC2 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 50296AD08284B473

FASTA34639,908
        10         20         30         40         50         60 
MKFAILFGGN SYEHEISIVS AVVLKKVINQ NLEFIFCDEE RRFYHIPSEK MNSKTFSTKA 

        70         80         90        100        110        120 
YKKEKELFIK QGGFFSKGFL KENKLECECV INLIHGRDGE DGKIAALFEF YSIKFIGPRL 

       130        140        150        160        170        180 
EASVLSFNKE LTKLYAKSVG VKTLDYTILR KNQNSKEKLS FPCIIKPARL GSSIGISIVK 

       190        200        210        220        230        240 
DEKDLEYAKD VGFEFDNDLV VEEFKNNIKE YNLAGCMIND EFVFSIIEEP KKKEFLDFEQ 

       250        260        270        280        290        300 
KYLSFSGHNE LIEADLSEEL KEKLKDSFKK IYNPLFKGAL IRCDFFILDN EIYLNEINPN 

       310        320        330        340 
PGSLANYLFK DFNTTLNALA DQISLEKMIK INYNFLHSIN GQKGKL 

« Hide

References

[1]"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences."
Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. expand/collapse author list , van Vliet A.H.M., Whitehead S., Barrell B.G.
Nature 403:665-668(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCTC 11168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL111168 Genomic DNA. Translation: CAL34926.1.
PIRF81351.
RefSeqYP_002344205.1. NC_002163.1.

3D structure databases

ProteinModelPortalQ9PPC2.
SMRQ9PPC2. Positions 1-323.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING192222.Cj0798c.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL34926; CAL34926; Cj0798c.
GeneID905128.
KEGGcje:Cj0798c.
PATRIC20058544. VBICamJej33762_0786.

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000102494.
KOK01921.
OMAHNGLLKN.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycCJEJ192222:GJTS-772-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_CAMJE
AccessionPrimary (citable) accession number: Q9PPC2
Secondary accession number(s): Q0PA92
Entry history
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways