Q9PPB8 (SYC_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	Cj0802

Organism

Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168) [Reference proteome] [HAMAP]

Taxonomic identifier

192222 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter › Campylobacter jejuni › Campylobacter jejuni subsp. jejuni

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP-Rule MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	Cysteine--tRNA ligase HAMAP-Rule MF_00041		PRO_0000159371
Regions
	Motif	26 – 36	11	"HIGH" region HAMAP-Rule MF_00041
	Motif	256 – 260	5	"KMSKS" region HAMAP-Rule MF_00041
Sites
	Metal binding	24	1	Zinc By similarity
	Metal binding	199	1	Zinc By similarity
	Metal binding	224	1	Zinc By similarity
	Metal binding	228	1	Zinc By similarity
	Binding site	259	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PPB8 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 1187E1252EDDEE89	FASTA	462	53,513
	10 20 30 40 50 60 MRLLDSVTKE KIKLDKKDIS IYLCGPTVYD DAHLGHARSS VCFDLLRRVL LAQGNRVKFA 70 80 90 100 110 120 RNYTDIDDKI LKKMAQSGQT LEEITEFYIK SYEEDMRVLN VLDPDFKPRA THYITAMLDL 130 140 150 160 170 180 IKKLAKDGFV YTLEDGIYFD TSKDEKYLSL SNRNLEENIS RLSNEVQKRN ESDFVLWKFD 190 200 210 220 230 240 ENFYESEFGK GRPGWHTECV AMIDSIFENT LDIHAGGIDL LFPHHENEAA QCRCGCKRKL 250 260 270 280 290 300 ANIWLHNGFV KIDGEKMSKS LNNSFFIKDA LKEFMGEALR FYLLSSHYRS HFNYSLSDLE 310 320 330 340 350 360 NAKKRLDKFY RLKKRLDLGE ISDFDVLNDI GIKSEIAKQI LEILNDDLNV SKALALLDDF 370 380 390 400 410 420 ISSANLELDK ESKNKILKQN IKEALSELAK IFGFGFMDAT LYFQWGVSKE EREEIEKLIL 430 440 450 460 ERTEAKKNKD FNTADAIREQ LNSKKITLLD TPNGTIWEKI NA

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL111168 Genomic DNA. Translation: CAL34930.1.
PIR	B81352.
RefSeq	YP_002344209.1. NC_002163.1.
3D structure databases
ProteinModelPortal	Q9PPB8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PPB8. 1 interaction.
STRING	192222.Cj0802.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAL34930; CAL34930; Cj0802.
GeneID	905105.
KEGG	cje:Cj0802.
PATRIC	20058552. VBICamJej33762_0790.
Phylogenomic databases
eggNOG	COG0215.
HOGENOM	HOG000245250.
KO	K01883.
OMA	DFDALNM.
ProtClustDB	PRK00260.
Enzyme and pathway databases
BioCyc	CJEJ192222:GJTS-776-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00041. Cys_tRNA_synth.
InterPro	IPR015803. Cys-tRNA-ligase. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR10890. PTHR10890. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_CAMJE

Accession

Primary (citable) accession number: Q9PPB8
Secondary accession number(s): Q0PA88

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program