ID   NADE_CAMJE              Reviewed;         246 AA.
AC   Q9PPB0; Q0PA80;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   16-JUN-2009, entry version 46.
DE   RecName: Full=NH(3)-dependent NAD(+) synthetase;
DE            EC=6.3.1.5;
GN   Name=nadE; OrderedLocusNames=Cj0810;
OS   Campylobacter jejuni.
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Campylobacteraceae; Campylobacter.
OX   NCBI_TaxID=197;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 11168 / Serotype O:2;
RX   MEDLINE=20150912; PubMed=10688204; DOI=10.1038/35001088;
RA   Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M.,
RA   Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S.,
RA   Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W.,
RA   Quail M.A., Rajandream M.A., Rutherford K.M., van Vliet A.H.M.,
RA   Whitehead S., Barrell B.G.;
RT   "The genome sequence of the food-borne pathogen Campylobacter jejuni
RT   reveals hypervariable sequences.";
RL   Nature 403:665-668(2000).
CC   -!- CATALYTIC ACTIVITY: ATP + deamido-NAD(+) + NH(3) = AMP +
CC       diphosphate + NAD(+).
CC   -!- PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from
CC       deamido-NAD(+) (ammonia route): step 1/1.
CC   -!- INTERACTION:
CC       Q0PC74:fliY; NbExp=1; IntAct=EBI-1193487, EBI-1191111;
CC   -!- SIMILARITY: Belongs to the NAD synthetase family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AL111168; CAL34938.1; -; Genomic_DNA.
DR   PIR; B81353; B81353.
DR   RefSeq; YP_002344217.1; -.
DR   HSSP; P08164; 1IH8.
DR   IntAct; Q9PPB0; 41.
DR   GeneID; 906011; -.
DR   GenomeReviews; AL111168_GR; Cj0810.
DR   KEGG; cje:Cj0810; -.
DR   OMA; Q9PPB0; CAINPIG.
DR   BioCyc; CJEJ192222:CJ0810-MON; -.
DR   BRENDA; 6.3.1.5; 255835.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0003952; F:NAD+ synthase (glutamine-hydrolyzing) activity; IEA:InterPro.
DR   GO; GO:0008795; F:NAD+ synthase activity; IEA:EC.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0009435; P:NAD biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00193; -; 1.
DR   InterPro; IPR003694; NAD_synthase.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   Pfam; PF02540; NAD_synthase; 1.
DR   TIGRFAMs; TIGR00552; nadE; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Complete proteome; Ligase; NAD; Nucleotide-binding.
FT   CHAIN         1    246       NH(3)-dependent NAD(+) synthetase.
FT                                /FTId=PRO_0000152162.
FT   NP_BIND      29     36       ATP (By similarity).
FT   ACT_SITE     31     31       By similarity.
SQ   SEQUENCE   246 AA;  27463 MW;  A92C822F968078EA CRC64;
     MDWQKITEKM CDFIQEKVKN SQSQGVVLGL SGGIDSALVA TLCKRALKEN VFALLMPTQI
     SNKANLEDAL RLCADLNLEY KIIEIQSILD AFIKQSENTT LVSLGNFAAR IRMSLLYDYS
     ALKNSLVIGT SNKSELLLGY GTIYGDLACA FNPIGSLYKS EIYALAKYLN LHENFIKKAP
     SADLWENQSD EADLGFSYTK IDEGLKALET NDEKLLRTLD PSLIAMLKNR MQKNAFKGKM
     PEILEI
//