Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.UniRule annotation

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NH(3)-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi35MagnesiumUniRule annotation1
Binding sitei110Deamido-NADUniRule annotation1
Binding sitei130ATPUniRule annotation1
Metal bindingi135MagnesiumUniRule annotation1
Binding sitei159ATPUniRule annotation1
Binding sitei181ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 36ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciCJEJ192222:G1G1F-776-MONOMER
UniPathwayiUPA00253; UER00333

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.1.5UniRule annotation)
Gene namesi
Name:nadEUniRule annotation
Ordered Locus Names:Cj0810
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001521621 – 246NH(3)-dependent NAD(+) synthetaseAdd BLAST246

Proteomic databases

PaxDbiQ9PPB0
PRIDEiQ9PPB0

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiQ9PPB0, 40 interactors
STRINGi192222.Cj0810

Structurei

Secondary structure

1246
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 19Combined sources17
Beta strandi24 – 29Combined sources6
Helixi34 – 47Combined sources14
Beta strandi50 – 55Combined sources6
Helixi64 – 76Combined sources13
Beta strandi79 – 82Combined sources4
Helixi86 – 93Combined sources8
Helixi101 – 122Combined sources22
Beta strandi125 – 128Combined sources4
Helixi133 – 138Combined sources6
Turni143 – 147Combined sources5
Beta strandi150 – 152Combined sources3
Turni153 – 156Combined sources4
Helixi159 – 168Combined sources10
Helixi173 – 177Combined sources5
Helixi200 – 209Combined sources10
Helixi213 – 218Combined sources6
Helixi221 – 233Combined sources13
Helixi235 – 238Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3P52X-ray2.74A/B1-246[»]
ProteinModelPortaliQ9PPB0
SMRiQ9PPB0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9PPB0

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4K Bacteria
COG0171 LUCA
HOGENOMiHOG000238069
KOiK01916
OMAiCAINPIG

Family and domain databases

CDDicd00553 NAD_synthase, 1 hit
Gene3Di3.40.50.620, 1 hit
HAMAPiMF_00193 NadE_ammonia_dep, 1 hit
InterProiView protein in InterPro
IPR022310 NAD/GMP_synthase
IPR003694 NAD_synthase
IPR022926 NH(3)-dep_NAD(+)_synth
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR23090 PTHR23090, 1 hit
PfamiView protein in Pfam
PF02540 NAD_synthase, 1 hit
TIGRFAMsiTIGR00552 nadE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9PPB0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDWQKITEKM CDFIQEKVKN SQSQGVVLGL SGGIDSALVA TLCKRALKEN
60 70 80 90 100
VFALLMPTQI SNKANLEDAL RLCADLNLEY KIIEIQSILD AFIKQSENTT
110 120 130 140 150
LVSLGNFAAR IRMSLLYDYS ALKNSLVIGT SNKSELLLGY GTIYGDLACA
160 170 180 190 200
FNPIGSLYKS EIYALAKYLN LHENFIKKAP SADLWENQSD EADLGFSYTK
210 220 230 240
IDEGLKALET NDEKLLRTLD PSLIAMLKNR MQKNAFKGKM PEILEI
Length:246
Mass (Da):27,463
Last modified:October 1, 2000 - v1
Checksum:iA92C822F968078EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA Translation: CAL34938.1
PIRiB81353
RefSeqiWP_002852555.1, NC_002163.1
YP_002344217.1, NC_002163.1

Genome annotation databases

EnsemblBacteriaiCAL34938; CAL34938; Cj0810
GeneIDi906011
KEGGicje:Cj0810
PATRICifig|192222.6.peg.798

Similar proteinsi

Entry informationi

Entry nameiNADE_CAMJE
AccessioniPrimary (citable) accession number: Q9PPB0
Secondary accession number(s): Q0PA80
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health