NH(3)-dependent NAD(+) synthetase - Campylobacter jejuni

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Q9PPB0 (NADE_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 64. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	Cj0810

Organism

Campylobacter jejuni

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD⁺ + NH₃ = AMP + diphosphate + NAD⁺. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
fliY	Q0PC74	1	EBI-1193487,EBI-1191111

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 246

246

NH(3)-dependent NAD(+) synthetase HAMAP MF_00193

PRO_0000152162

Regions

Nucleotide binding

29 – 36

ATP By similarity

Sites

Active site

By similarity

Secondary structure

246

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q9PPB0 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: A92C822F968078EA
Show »

FASTA

246

27,463

        10         20         30         40         50         60 
MDWQKITEKM CDFIQEKVKN SQSQGVVLGL SGGIDSALVA TLCKRALKEN VFALLMPTQI 

        70         80         90        100        110        120 
SNKANLEDAL RLCADLNLEY KIIEIQSILD AFIKQSENTT LVSLGNFAAR IRMSLLYDYS 

       130        140        150        160        170        180 
ALKNSLVIGT SNKSELLLGY GTIYGDLACA FNPIGSLYKS EIYALAKYLN LHENFIKKAP 

       190        200        210        220        230        240 
SADLWENQSD EADLGFSYTK IDEGLKALET NDEKLLRTLD PSLIAMLKNR MQKNAFKGKM 


PEILEI

« Hide

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences."
Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M.

Barrell B.G.
Nature 403:665-668(2000) [PubMed: 10688204] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCTC 11168 / Serotype O:2.

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AL111168 Genomic DNA. Translation: CAL34938.1.

PIR

B81353.

RefSeq

YP_002344217.1. NC_002163.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3P52	X-ray	2.74	A/B	1-246	[»]

ProteinModelPortal

Q9PPB0.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9PPB0. 42 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

906011.

GenomeReviews

Gene locus Cj0810 in contig AL111168_GR.

KEGG

cje:Cj0810.

PATRIC

20058568. VBICamJej33762_0798.

Phylogenomic databases

HOGENOM

HBG351567.

OMA

YDISARD.

ProtClustDB

PRK13980.

Enzyme and pathway databases

BioCyc

CJEJ192222:CJ0810-MONOMER.

Family and domain databases

HAMAP

MF_00193. NadE.
[Tree]

InterPro

IPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]

Gene3D

G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.

K01916.

Pfam

PF02540. NAD_synthase. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00552. NadE. 1 hit.

ProtoNet

Search...

Entry information

Entry name

NADE_CAMJE

Accession

Primary (citable) accession number: Q9PPB0
Secondary accession number(s): Q0PA80

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents