Reviewed, UniProtKB/Swiss-Prot Q9PP34 (SYFA_CAMJE)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	Cj0897

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00281
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_0000126681
Sites
	Metal binding	246	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PP34-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 02013BD56B6AEAF2	FASTA	330	37,987
	10 20 30 40 50 60 MQNFIEQIQK CENLNDLEAI RISVLGKKGI LTEGFTKLKE LEDEAKKEFA AKLNAQKEIF 70 80 90 100 110 120 NEAYLAKFKD LENLALEERM KQDALNFNYF DESITTGALH PVMSTMDKII EYFIALNFSI 130 140 150 160 170 180 EKGPLIEDDF HNFEALNLPK SHPARDMQDT FYFDDKRLLR TQTSPVQIRT MLAQKPPIRM 190 200 210 220 230 240 IAPGAVFRRD FDITHTPMFH QVEGLVVEEG QKVSFANLKS VLEDFLRYMF GDVKVRFRPS 250 260 270 280 290 300 FFPFTEPSAE VDISCVFCKG KGCRVCKHTG WLEVLGCGIV DPNVYNFVGY ENVSGYAFGL 310 320 330 GVERFAMLLH QIPDLRSLFE GDLRLLEQFR

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL111168 Genomic DNA. Translation: CAL35018.1.
PIR	A81363.
RefSeq	YP_002344296.1.
3D structure databases
HSSP	HSSP built from PDB template 1JJC based on UniProtKB P27001.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PP34. 12 interactions.
Genome annotation databases
GeneID	905193.
GenomeReviews	Gene locus Cj0897 in contig AL111168_GR.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q9PP34. FRASYFP.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0897C-MON.
BRENDA	6.1.1.20. 255835.
Family and domain databases
HAMAP	MF_00281. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR002319. Phe-tRNA-synth_IIc-rel. IPR004529. Phe-tRNA-synth_IIc_asu. IPR018157. Phe-tRNA-synth_IIc_C. IPR004188. Phe-tRNA_synth_II_N. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_CAMJE

Accession

Primary (citable) accession number: Q9PP34
Secondary accession number(s): Q0PA01

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)