Reviewed, UniProtKB/Swiss-Prot Q9PP15 (KPRS_CAMJE)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase
      Short name=RPPK
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase
      Short name=P-Rib-PP synthetase
      Short name=PRPP synthetase

Gene names

Name:	prs
Synonyms:	prsA
Ordered Locus Names:	Cj0918c

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Ribose-phosphate pyrophosphokinase HAMAP MF_00583		PRO_0000141121
Regions
	Region	211 – 224	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	128	1	Magnesium Potential
	Metal binding	130	1	Magnesium Potential
	Metal binding	139	1	Magnesium Potential
	Metal binding	143	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9PP15-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 3CC90CD74E69A433	FASTA	309	33,698
	10 20 30 40 50 60 MRGYKIFSGS ANVEFARQVS KYLSLPLSDA GVKRFSDGEI SVQIDESVRG KDVFIIQSTC 70 80 90 100 110 120 APTNDNLMEL LILTDALRRS SANSITAIIP YFGYARQDRK ANPRVPITAK LVANLIQAAG 130 140 150 160 170 180 IDRVATIDLH AGQIQGFFDI PVDNLYGSIV FNDYIKAKHF KNAIIGSPDI GGVARARSVA 190 200 210 220 230 240 KHLGLDIVIV DKRREKANES EVMNIIGDVK DKEVILVDDI IDTAGTIVKA AEALKEKGAK 250 260 270 280 290 300 SVMACCTHAV LSGKAYERIA SGVLDELVVT DTIPLKEQLP NIKVLSVTPV FAEVIRRVYH NESVNSLFI

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL111168 Genomic DNA. Translation: CAL35038.1.
PIR	E81365.
RefSeq	YP_002344316.1.
3D structure databases
HSSP	HSSP built from PDB template 1DKU based on UniProtKB P14193.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PP15. 79 interactions.
Genome annotation databases
GeneID	905217.
GenomeReviews	Gene locus Cj0918c in contig AL111168_GR.
KEGG	cje:Cj0918c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NVAEIMN.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0918C-MON.
BRENDA	2.7.6.1. 255835.
Family and domain databases
HAMAP	MF_00583. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_CAMJE

Accession

Primary (citable) accession number: Q9PP15
Secondary accession number(s): Q0P9Y1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)