Q9PP04 (AMPA_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	Cj0929

Organism

Campylobacter jejuni

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 483	483	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_0000165734
Sites
	Active site	256	1	Potential
	Active site	330	1	Potential
	Metal binding	244	1	Manganese 2 By similarity
	Metal binding	249	1	Manganese 1 By similarity
	Metal binding	249	1	Manganese 2 By similarity
	Metal binding	267	1	Manganese 2 By similarity
	Metal binding	326	1	Manganese 1 By similarity
	Metal binding	328	1	Manganese 1 By similarity
	Metal binding	328	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PP04 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: A362874369ADDE6C	FASTA	483	53,694
	10 20 30 40 50 60 MKFELNDKKL DAIKADFELV FIQDKNLKIF NKEKDFFKLN NYKGEGALLD LNNKKLYLEL 70 80 90 100 110 120 KSLAYEDIRL SLCTAYKTLE KLNIKSVKLP SIIGDCVVRS FASLVEGVLF GAYKFDKYKS 130 140 150 160 170 180 EKKTSTLEKF IISNEELNGK KFNKDEAKIG LERGEILANA TNFTKNIVNE IPEIYTPLKM 190 200 210 220 230 240 AEDAQNLAKE NKNIICKIYD EKFLAKEKMN AFLAVNHASV HPPRLIHLSY KAKNAKKRVV 250 260 270 280 290 300 FVGKGLTYDS GGLSLKPADF MLTMKADKSG AAAAMGIIKA VAELALDLEV HCILGATENM 310 320 330 340 350 360 IGGNAYKPDD VLISREGVSI EVRNTDAEGR LVLADCLSFA QDLKPDLLID MATLTGACVV 370 380 390 400 410 420 GLGEFTSAIM GNNEELQNDF YLSSKKSGEY TTILHFNPHL RELIKSNIAD VSNTASSRYG 430 440 450 460 470 480 GAITAGLFLD KFIRKEYKDK WLHLDIAGPA YTEKSWGYSS FGAGGAGVRM CVNYLIQILR KAK

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL111168 Genomic DNA. Translation: CAL35049.1.
PIR	H81366.
RefSeq	YP_002344327.1. NC_002163.1.
3D structure databases
ProteinModelPortal	Q9PP04.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PP04. 9 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	904492.
GenomeReviews	Gene locus Cj0929 in contig AL111168_GR.
KEGG	cje:Cj0929.
PATRIC	20058802. VBICamJej33762_0913.
Phylogenomic databases
HOGENOM	HBG742580.
OMA	NMHLMRY.
ProtClustDB	PRK00913.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0929-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_CAMJE

Accession

Primary (citable) accession number: Q9PP04
Secondary accession number(s): Q0P9X0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program