Reviewed, UniProtKB/Swiss-Prot Q9PNY0 (PURL_CAMJE)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase 2
    EC=6.3.5.3
Alternative name(s):
    Phosphoribosylformylglycinamidine synthase II
      Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Cj0955

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/5. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, purQ and purL. HAMAP MF_00420
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
flaG	Q0PAW9	1	EBI-1192636,EBI-1191365

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 728	728	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100446
Regions
	Nucleotide binding	99 – 110	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9PNY0-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: A7CAD4432EDF678A	FASTA	728	79,310
	10 20 30 40 50 60 MDKETIKAHK ISDEEYAQIL EILGREPNLL ELGVISAMWS EHCSYKSSKK YLNGFPTKAP 70 80 90 100 110 120 WVIQGPGENA GVIDIGQGMA AVFKVESHNH PSFIEPFAGA ATGVGGILRD VFTMGARVVA 130 140 150 160 170 180 GLNSLKFGDI HDEKCGKHQK YLVKGVVNGI SHYGNCMGVP TIGGECAFDE CFNGNILVNA 190 200 210 220 230 240 FALGVCKSED IFYAKAEGVG NPVIYVGSKT GRDGLGGAVM ASDSFNEESK SLRPTVQIGD 250 260 270 280 290 300 PFSEKLLMEA CLELFKTDYI VGIQDMGAAG LTSSSFEMAG RSGSGMKLYL DKTPMRESGM 310 320 330 340 350 360 TPYELMLSES QERMLICAKK GYEDKVIEIF KKWDLDAVVM GEVTNTGKME LFWHDELVGL 370 380 390 400 410 420 IPIEPLSEKA PILSRPTSEP KYLSEIKNYK FELKSSVQEL FIQMLQNENI NNKAFIYDQF 430 440 450 460 470 480 DSSVQTNTIK ADGRLGASVI RIKENGASVA MAIECNSRLN YVNSKIGAAL AVASAGRKVA 490 500 510 520 530 540 CTGAKPLAIS DCLNYGNPQN PEVMWQFAQG CEGIKEACKE LNTPVVSGNV SLYNETEGVS 550 560 570 580 590 600 IYPSPTIVSV GVLEDANKTL KASFEKENLS VYLLGESLGE FSGSMVMKIQ DKKVSGSLKE 610 620 630 640 650 660 LDYKAELALW DLLYKANQNS LLECANSVGI GGIAMTLAKM FAISSVGANL TSDFDDEKMI 670 680 690 700 710 720 FDESASRAII GLSKENEEAF LNLAKEFGVK AYKLGVSTSQ KHFKLDSIEL SKAELDKLYF ESFKEQIQ

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL111168 Genomic DNA. Translation: CAL35075.1.
PIR	B81370.
RefSeq	YP_002344353.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PNY0. 3 interactions.
Genome annotation databases
GeneID	904957.
GenomeReviews	Gene locus Cj0955 in contig AL111168_GR.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q9PNY0. GNCVGVP.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0955C-MON.
BRENDA	6.3.5.3. 255835.
Family and domain databases
HAMAP	MF_00420. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_CAMJE

Accession

Primary (citable) accession number: Q9PNY0
Secondary accession number(s): Q0P9U4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)