Q9PNY0 (PURL_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Cj0955

Organism

Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168) [Reference proteome] [HAMAP]

Taxonomic identifier

192222 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter › Campylobacter jejuni › Campylobacter jejuni subsp. jejuni

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP-Rule MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP-Rule MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
flaG	Q0PAW9	1	EBI-1192636,EBI-1191365

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 728	728	Phosphoribosylformylglycinamidine synthase 2 HAMAP-Rule MF_00420		PRO_0000100446
Regions
	Nucleotide binding	99 – 110	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9PNY0 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: A7CAD4432EDF678A	FASTA	728	79,310
	10 20 30 40 50 60 MDKETIKAHK ISDEEYAQIL EILGREPNLL ELGVISAMWS EHCSYKSSKK YLNGFPTKAP 70 80 90 100 110 120 WVIQGPGENA GVIDIGQGMA AVFKVESHNH PSFIEPFAGA ATGVGGILRD VFTMGARVVA 130 140 150 160 170 180 GLNSLKFGDI HDEKCGKHQK YLVKGVVNGI SHYGNCMGVP TIGGECAFDE CFNGNILVNA 190 200 210 220 230 240 FALGVCKSED IFYAKAEGVG NPVIYVGSKT GRDGLGGAVM ASDSFNEESK SLRPTVQIGD 250 260 270 280 290 300 PFSEKLLMEA CLELFKTDYI VGIQDMGAAG LTSSSFEMAG RSGSGMKLYL DKTPMRESGM 310 320 330 340 350 360 TPYELMLSES QERMLICAKK GYEDKVIEIF KKWDLDAVVM GEVTNTGKME LFWHDELVGL 370 380 390 400 410 420 IPIEPLSEKA PILSRPTSEP KYLSEIKNYK FELKSSVQEL FIQMLQNENI NNKAFIYDQF 430 440 450 460 470 480 DSSVQTNTIK ADGRLGASVI RIKENGASVA MAIECNSRLN YVNSKIGAAL AVASAGRKVA 490 500 510 520 530 540 CTGAKPLAIS DCLNYGNPQN PEVMWQFAQG CEGIKEACKE LNTPVVSGNV SLYNETEGVS 550 560 570 580 590 600 IYPSPTIVSV GVLEDANKTL KASFEKENLS VYLLGESLGE FSGSMVMKIQ DKKVSGSLKE 610 620 630 640 650 660 LDYKAELALW DLLYKANQNS LLECANSVGI GGIAMTLAKM FAISSVGANL TSDFDDEKMI 670 680 690 700 710 720 FDESASRAII GLSKENEEAF LNLAKEFGVK AYKLGVSTSQ KHFKLDSIEL SKAELDKLYF ESFKEQIQ

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL111168 Genomic DNA. Translation: CAL35075.1.
PIR	B81370.
RefSeq	YP_002344353.1. NC_002163.1.
3D structure databases
ProteinModelPortal	Q9PNY0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PNY0. 3 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAL35075; CAL35075; Cj0955c.
GeneID	904957.
KEGG	cje:Cj0955c.
PATRIC	20058870. VBICamJej33762_0939.
Phylogenomic databases
eggNOG	COG0046.
HOGENOM	HOG000238227.
KO	K01952.
OMA	WSEHCCY.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	CJEJ192222:GJTS-930-MONOMER.
UniPathway	UPA00074; UER00128.
Family and domain databases
HAMAP	MF_00420. PurL_2.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_CAMJE

Accession

Primary (citable) accession number: Q9PNY0
Secondary accession number(s): Q0P9U4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program