Reviewed, UniProtKB/Swiss-Prot Q9PNU6 (OTC_CAMJE)

Names and origin

Protein names

Recommended name:
    Ornithine carbamoyltransferase
      Short name=OTCase
    EC=2.1.3.3

Gene names

Name:	argF
Ordered Locus Names:	Cj0994c

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3. HAMAP MF_01109
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: HAMAP protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
flaG	Q0PAW9	1	EBI-1195069,EBI-1191365

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Ornithine carbamoyltransferase HAMAP MF_01109		PRO_0000112904
Regions
	Region	46 – 50	5	Carbamoyl phosphate binding By similarity
	Region	259 – 262	4	Ornithine binding By similarity
Sites
	Binding site	97	1	Carbamoyl phosphate By similarity
	Binding site	124	1	Carbamoyl phosphate By similarity
	Site	26	1	Important for structural integrity By similarity
	Site	137	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PNU6-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 58CBF72AF8C86116	FASTA	306	34,976
	10 20 30 40 50 60 MKHFLTLRDF SKEEILSLVN HASELKKEPK KLLQDKTLAM IFEKNSTRTR MAFELAITEL 70 80 90 100 110 120 GGKALFLSSN DLQLSRGEPV KDTARVIGAM VDFVMMRVNK HETLLEFARY SKAPVINALS 130 140 150 160 170 180 ELYHPTQVLG DLFTIKEWNK MQNGIAKVAF IGDSNNMCNS WLITAAILGF EISIAMPKNY 190 200 210 220 230 240 KISPEIWEFA MKQALISGAK ISLGYDKFEA LKDKDVVITD TWVSMGEENE KERKIKEFEG 250 260 270 280 290 300 FMIDEKAMSV ANKDAILLHC LPAYRGYEVS EEIFEKHADV IFEEARNRLY VVKALLCFLD NQRGRE

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL111168 Genomic DNA. Translation: CAL35112.1.
PIR	G81374.
RefSeq	YP_002344389.1.
3D structure databases
HSSP	HSSP built from PDB template 1A1S based on UniProtKB Q51742.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PNU6. 69 interactions.
Genome annotation databases
GeneID	905285.
GenomeReviews	Gene locus Cj0994c in contig AL111168_GR.
KEGG	cje:Cj0994c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q9PNU6. KHETLLE.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0994C-MON.
BRENDA	2.1.3.3. 255835.
Family and domain databases
HAMAP	MF_01109. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P_bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn_bd. IPR002292. Orn_carbamltrans. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
TIGRFAMs	TIGR00658. orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTC_CAMJE

Accession

Primary (citable) accession number: Q9PNU6
Secondary accession number(s): Q0P9Q8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	October 1, 2000
Last modified:	May 5, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)