Reviewed, UniProtKB/Swiss-Prot Q9PNT0 (TGT_CAMJE)

Names and origin

Protein names

Recommended name:
    Queuine tRNA-ribosyltransferase
    EC=2.4.2.29
Alternative name(s):
    tRNA-guanine transglycosylase
    Guanine insertion enzyme

Gene names

Name:	tgt
Ordered Locus Names:	Cj1010

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.
Catalytic activity	[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168 [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00168
Sequence similarities	Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	protein binding Inferred from physical interaction. Source: IntAct queuine tRNA-ribosyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
flgG2	Q0PAI2	1	EBI-1192693,EBI-1191173

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 373	373	Queuine tRNA-ribosyltransferase HAMAP MF_00168		PRO_0000135459
Sites
	Active site	90	1	Nucleophile By similarity
	Metal binding	308	1	Zinc By similarity
	Metal binding	310	1	Zinc By similarity
	Metal binding	313	1	Zinc By similarity
	Metal binding	339	1	Zinc By similarity
	Binding site	91	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PNT0-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 8C197BF939898CCA	FASTA	373	42,574
	10 20 30 40 50 60 MEFKLKHKDG MARVCEITTA HSTFLTPVFM PVGTVGAVKS LDANDMKNEL DAKIILANTY 70 80 90 100 110 120 HMYLRPTSKV VKDFGGLHGF TKFDRSFLTD SGGFQAFSLS KNSKHFNEGI EFKSHIDGSR 130 140 150 160 170 180 HLFTPKSVLD TQYDFNSDIM MILDDLVALP ATKERVKISV DRTILWAKEA ITYHKNMQNK 190 200 210 220 230 240 GIGIGQNIFG IIQGGTDYEE RKRCALSLNE MPFDGLAIGG LSVGEENALM YETVQNLNPY 250 260 270 280 290 300 LDENRPRYLM GVGTPEDLVE NVERGVDMFD CVMPTRNARN GTFFTSFGKF NIKKAEFIND 310 320 330 340 350 360 HEVIDSTCSC YTCRNFSRGY LNHLFKAKEL TFFRLASLHN LHYYLELARK MREAILNNSF 370 TQFKRNFYHL RGK

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL111168 Genomic DNA. Translation: CAL35128.1.
PIR	G81302.
RefSeq	YP_002344405.1.
3D structure databases
HSSP	HSSP built from PDB template 1K4G based on UniProtKB P28720.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PNT0. 6 interactions.
Genome annotation databases
GeneID	905301.
GenomeReviews	Gene locus Cj1010 in contig AL111168_GR.
KEGG	cje:Cj1010.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q9PNT0. ECVRLPA.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ1010-MON.
BRENDA	2.4.2.29. 255835.
Family and domain databases
HAMAP	MF_00168. [Tree]
InterPro	IPR004803. QtRNA_ribo_trans. IPR002616. tRNA_ribo_trans. [Graphical view]
Gene3D	G3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
PANTHER	PTHR11962. tRNA_ribo_trans. 1 hit.
Pfam	PF01702. TGT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00430. Q_tRNA_tgt. 1 hit. TIGR00449. tgt_general. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TGT_CAMJE

Accession

Primary (citable) accession number: Q9PNT0
Secondary accession number(s): Q0P9P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)