Reviewed, UniProtKB/Swiss-Prot Q9PNN7 (MURC_CAMJE)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	Cj1054c

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
flaG	Q0PAW9	1	EBI-1194955,EBI-1191365

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000182073
Regions
	Nucleotide binding	109 – 115	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9PNN7-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E7130AAA56D9AFBD	FASTA	432	48,372
	10 20 30 40 50 60 MMQNIHFIGI GGIGISALAR FLREKGFKIS GSDLKESKIT KELEKEGVKV SIPHHKDNIL 70 80 90 100 110 120 NKDLVIYSAA IKEENPEFKY AKELGIKCLS RKEALPLILE DKRVFAVAGA HGKSTTSSIL 130 140 150 160 170 180 ASLLDDASVI IGAILKEFGS NMIYKESQNL VFEADESDSS FLNSNPYLAI VTNAEAEHLD 190 200 210 220 230 240 HYGNEVSKLH HAYTQFLDVA KIRVINAEDE FLKNYKNESI KLYPSKDIKN CTMCIENFKP 250 260 270 280 290 300 FTSFELKDLG EFKVFGMGYH LALDASLAIL AALNFLDIET IRTRLKNYQG IKKRFDILHA 310 320 330 340 350 360 DENLVLIDDY GHHPTEIKAT LSAAQEYVKL GGYKKITAIF EPHRYTRLAT NLKEFAKAFE 370 380 390 400 410 420 GVDELVILPV YAAGEEPIEL DLKAVFPKAL FVEDIKREGK FLVASKGQVF EEGLIIGFGA 430 GDISNKLRQK NE

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL111168 Genomic DNA. Translation: CAL35172.1.
PIR	C81308.
RefSeq	YP_002344449.1.
3D structure databases
HSSP	HSSP built from PDB template 1GQY based on UniProtKB P45066.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PNN7. 22 interactions.
Genome annotation databases
GeneID	905346.
GenomeReviews	Gene locus Cj1054c in contig AL111168_GR.
KEGG	cje:Cj1054c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q9PNN7. EWMVVEA.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ1054C-MON.
BRENDA	6.3.2.8. 255835.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_CAMJE

Accession

Primary (citable) accession number: Q9PNN7
Secondary accession number(s): Q0P9J8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)