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Q9PNK3 (SYL_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Cj1091
OrganismCampylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168) [Reference proteome] [HAMAP]
Taxonomic identifier192222 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Protein attributes

Sequence length809 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 809809Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000151992

Regions

Motif40 – 5011"HIGH" region HAMAP-Rule MF_00049
Motif579 – 5835"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5821ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9PNK3 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: D7300CDA3DEF8C39

FASTA80993,431
        10         20         30         40         50         60 
MAYEASLIEK KWQKIWDENE YFEPKDDLNL PKKYILSMFP YPSGRIHMGH VRNYTIGDAL 

        70         80         90        100        110        120 
ARYYRKIGFN VLHPIGFDSF GMPAENAAIK HKIHPKSWTY ENIAYMKKEL FSLGFSFSKK 

       130        140        150        160        170        180 
RMLATSDPLY TKFEQEFFIK MFEKGLIYTK EANVNWCEQD QTVLANEQVE DGKCWRCGHE 

       190        200        210        220        230        240 
VVQKKMPGYY VKITAYAEEL LKDLEELKDK WPNQVLTMQE NWIGKSEGLE FSLNLDEESK 

       250        260        270        280        290        300 
QKTKESSLEV FTTRADTIYG VSYIALAPEH KIVQNLLSQN LLNQDVLNKI KVIQNQSPRE 

       310        320        330        340        350        360 
RQSSEKEGYF LGIYAIHPLS GEKIPLWVAN FVLADYGSGA VMAVPAHDER DFEFATKYNL 

       370        380        390        400        410        420 
AIKQVIQTQE NLPYMQKLGK LINSQEFDNL DCNEARLKII SQFEAKNIGK RVVNFKIRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPMIKCQSC GIVPQKLENL PITLPEDVQI TGEGNPLDKH PTWKNCICPK 

       490        500        510        520        530        540 
CGKEAQKESD TLDTFFESSW YFARFASDEK TWQEKALDEK SVKYWMSVDQ YIGGIEHAIL 

       550        560        570        580        590        600 
HLLYARFFQK ALRDLGYLTQ NEPFDRLLTQ GMVLKDGAKM SKSKGNVVDP DEIIEKYGAD 

       610        620        630        640        650        660 
TARLFILFAA PPAKELEWND DAVEGAYRFI CKLYDRAQNV KKGELVELKQ ENLNKEEKYA 

       670        680        690        700        710        720 
RLKVYEALKK SFEVYHQSFA FNTLIAACME ALNALALCKN EALEQEAFYI ILNILEPIIP 

       730        740        750        760        770        780 
HVCFELSEEL FKCKNFKKLE LKEEVFVKDT LNLAVSINGK KRAEFEISSS ASKEEILAFA 

       790        800 
KENTAKWLEG KSIVKEIYVE GKLVNLVIK 

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References

[1]"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences."
Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. expand/collapse author list , van Vliet A.H.M., Whitehead S., Barrell B.G.
Nature 403:665-668(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCTC 11168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL111168 Genomic DNA. Translation: CAL35208.1.
PIRF81312.
RefSeqYP_002344484.1. NC_002163.1.

3D structure databases

ProteinModelPortalQ9PNK3.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL35208; CAL35208; Cj1091c.
GeneID905382.
KEGGcje:Cj1091c.
PATRIC20059140. VBICamJej33762_1073.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycCJEJ192222:GJTS-1064-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CAMJE
AccessionPrimary (citable) accession number: Q9PNK3
Secondary accession number(s): Q0P9G3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries