Reviewed, UniProtKB/Swiss-Prot Q9PNE6 (GMHA1_CAMJE)

Names and origin

Protein names

Recommended name:
    Phosphoheptose isomerase 1
    EC=5.3.1.-
Alternative name(s):
    Sedoheptulose 7-phosphate isomerase 1

Gene names

Name:	gmhA1
Synonyms:	gmhA
Ordered Locus Names:	Cj1149c

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate By similarity.
Catalytic activity	D-sedoheptulose 7-phosphate = D-glycero-alpha-D-manno-heptose 7-phosphate + D-glycero-beta-D-manno-heptose 7-phosphate. HAMAP MF_00067
Pathway	Carbohydrate biosynthesis; D-glycero-D-manno-heptose biosynthesis; D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate: step 1/1. HAMAP MF_00067 Bacterial outer membrane biogenesis; LOS core biosynthesis. HAMAP MF_00067
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the SIS family. GmhA subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide core region biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	D-sedoheptulose 7-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP protein binding Inferred from physical interaction. Source: IntAct sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
fliH	Q0PBI9	1	EBI-1193890,EBI-1191439
fliY	Q0PC74	1	EBI-1193890,EBI-1191111

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 186

186

Phosphoheptose isomerase 1 HAMAP MF_00067

PRO_0000136522

Experimental info

Sequence conflict

16

1

V → A Ref.3

Sequence conflict

18

1

A → E Ref.1

Sequence conflict

18

1

A → E Ref.2

Sequence conflict

18

1

A → E Ref.3

Sequence conflict

31

1

E → D Ref.1

Sequence conflict

31

1

E → D Ref.2

Sequence conflict

109

1

K → N Ref.1

Sequence conflict

109

1

K → N Ref.2

Sequence conflict

109

1

K → N Ref.3

Sequence conflict

128

1

L → F Ref.1

Sequence conflict

128

1

L → F Ref.2

Sequence conflict

128

1

L → F Ref.3

Secondary structure

1

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186

Helix Strand Turn

Details...

Helix

2 – 18

17

Helix

19 – 22

4

Helix

23 – 38

16

Beta strand

43 – 48

6

Helix

50 – 63

14

Beta strand

66 – 68

3

Beta strand

75 – 79

5

Helix

82 – 91

10

Helix

94 – 96

3

Helix

97 – 105

9

Beta strand

111 – 115

5

Beta strand

117 – 119

3

Helix

122 – 133

12

Beta strand

137 – 143

7

Helix

144 – 148

5

Helix

149 – 152

4

Beta strand

154 – 160

7

Helix

164 – 185

22

Sequences

	Sequence		Length	Mass (Da)
	Q9PNE6-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: D7759471EC88924D	FASTA	186	20,085
	10 20 30 40 50 60 MINLVEKEWQ EHQKIVQASE ILKGQIAKVG ELLCECLKKG GKILICGNGG SAADAQHFAA 70 80 90 100 110 120 ELSGRYKKER KALAGIALTT DTSALSAIGN DYGFEFVFSR QVEALGNEKD VLIGISTSGK 130 140 150 160 170 180 SPNVLEALKK AKELNMLCLG LSGKGGGMMN KLCDHNLVVP SDDTARIQEM HILIIHTLCQ IIDESF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biosynthesis of ganglioside mimics in Campylobacter jejuni OH4384. Identification of the glycosyltransferase genes, enzymatic synthesis of model compounds, and characterization of nanomole amounts by 600-MHz (1)H and (13)C NMR analysis." Gilbert M., Brisson J.-R., Karwaski M.-F., Michniewicz J., Cunningham A.-M., Wu Y., Young N.M., Wakarchuk W.W. J. Biol. Chem. 275:3896-3906(2000) [PubMed: 10660542] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43446 / MK104 / Serotype O:19.
[2]	Gilbert M. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Evidence for acquisition of the lipooligosaccharide biosynthesis locus in Campylobacter jejuni GB11, a strain isolated from a patient with Guillain-Barre syndrome, by horizontal exchange." Gilbert M., Godschalk P.C., Karwaski M.-F., Ang C.W., Van Belkum A., Li J., Wakarchuk W.W., Endtz H.P. Infect. Immun. 72:1162-1165(2004) [PubMed: 14742567] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: GB11.
[4]	"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.
[5]	"Novel pathways for biosynthesis of nucleotide-activated glycero-manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides." Valvano M.A., Messner P., Kosma P. Microbiology 148:1979-1989(2002) [PubMed: 12101286] [Abstract] Cited for: BIOSYNTHESIS OF NUCLEOTIDE-ACTIVATED GLYCERO-MANNO-HEPTOSE.
[6]	"Deciphering Campylobacter jejuni cell surface interactions from the genome sequence." Linton D., Karlyshev A.V., Wren B.W. Curr. Opin. Microbiol. 4:35-40(2001) [PubMed: 11173031] [Abstract] Cited for: CELL SURFACE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF167344 Genomic DNA. Translation: AAR99164.1.
AY422197 Genomic DNA. Translation: AAR82882.1.
AL111168 Genomic DNA. Translation: CAL35264.1.

PIR

G81319.

RefSeq

YP_002344540.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1TK9	X-ray	2.10	A/B/C/D	2-186	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9PNE6. 105 interactions.

Genome annotation databases

GeneID

905439.

GenomeReviews

Gene locus Cj1149c in contig AL111168_GR.

KEGG

cje:Cj1149c.

Organism-specific databases

CMR

Search...

Phylogenomic databases

OMA

Q9PNE6. ANDLGYD.

Enzyme and pathway databases

BioCyc

CJEJ192222:CJ1149C-MON.

Family and domain databases

HAMAP

MF_00067.
[Tree]

InterPro

IPR004515. GmhA.
IPR001347. SIS.
[Graphical view]

Pfam

PF01380. SIS. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00441. gmhA. 1 hit.

ProtoNet

Search...

Entry information

Entry name

GMHA1_CAMJE

Accession

Primary (citable) accession number: Q9PNE6
Secondary accession number(s): Q0P9A7, Q6TG10, Q7BPS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 28, 2003
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)