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Protein

Phosphoheptose isomerase 1

Gene

gmhA1

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate.By similarity

Catalytic activityi

D-sedoheptulose 7-phosphate = D-glycero-D-manno-heptose 7-phosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: D-glycero-D-manno-heptose 7-phosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Phosphoheptose isomerase 1 (gmhA1), Phosphoheptose isomerase 2 (gmhA2)
This subpathway is part of the pathway D-glycero-D-manno-heptose 7-phosphate biosynthesis, which is itself part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate, the pathway D-glycero-D-manno-heptose 7-phosphate biosynthesis and in Carbohydrate biosynthesis.

Pathwayi: LOS core biosynthesis

This protein is involved in the pathway LOS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LOS core biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi57ZincBy similarity1
Metal bindingi61ZincBy similarity1
Binding sitei61SubstrateBy similarity1
Binding sitei121SubstrateBy similarity1
Metal bindingi168ZincBy similarity1
Binding sitei168SubstrateBy similarity1
Metal bindingi176ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-1076-MONOMER.
BRENDAi5.3.1.28. 1087.
UniPathwayiUPA00041; UER00436.
UPA00976.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoheptose isomerase 1 (EC:5.3.1.28)
Alternative name(s):
Sedoheptulose 7-phosphate isomerase 1
Gene namesi
Name:gmhA1
Synonyms:gmhA
Ordered Locus Names:Cj1149c
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001365221 – 186Phosphoheptose isomerase 1Add BLAST186

Proteomic databases

PaxDbiQ9PNE6.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiQ9PNE6. 103 interactors.
STRINGi192222.Cj1149c.

Structurei

Secondary structure

1186
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 18Combined sources17
Helixi19 – 22Combined sources4
Helixi23 – 38Combined sources16
Beta strandi43 – 48Combined sources6
Helixi50 – 63Combined sources14
Beta strandi66 – 68Combined sources3
Beta strandi75 – 79Combined sources5
Helixi82 – 91Combined sources10
Helixi94 – 96Combined sources3
Helixi97 – 105Combined sources9
Beta strandi111 – 115Combined sources5
Beta strandi117 – 119Combined sources3
Helixi122 – 133Combined sources12
Beta strandi137 – 143Combined sources7
Helixi144 – 148Combined sources5
Helixi149 – 152Combined sources4
Beta strandi154 – 160Combined sources7
Helixi164 – 185Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TK9X-ray2.10A/B/C/D2-186[»]
ProteinModelPortaliQ9PNE6.
SMRiQ9PNE6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9PNE6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 186SISAdd BLAST154

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni48 – 50Substrate bindingBy similarity3
Regioni90 – 91Substrate bindingBy similarity2
Regioni116 – 118Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the SIS family. GmhA subfamily.Curated
Contains 1 SIS domain.Curated

Phylogenomic databases

eggNOGiENOG4105F55. Bacteria.
COG0279. LUCA.
HOGENOMiHOG000237571.
KOiK03271.
OMAiEMHILMI.

Family and domain databases

HAMAPiMF_00067. GmhA. 1 hit.
InterProiIPR004515. Phosphoheptose_Isoase.
IPR001347. SIS.
[Graphical view]
PfamiPF13580. SIS_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00441. gmhA. 1 hit.
PROSITEiPS51464. SIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PNE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINLVEKEWQ EHQKIVQASE ILKGQIAKVG ELLCECLKKG GKILICGNGG
60 70 80 90 100
SAADAQHFAA ELSGRYKKER KALAGIALTT DTSALSAIGN DYGFEFVFSR
110 120 130 140 150
QVEALGNEKD VLIGISTSGK SPNVLEALKK AKELNMLCLG LSGKGGGMMN
160 170 180
KLCDHNLVVP SDDTARIQEM HILIIHTLCQ IIDESF
Length:186
Mass (Da):20,085
Last modified:October 1, 2000 - v1
Checksum:iD7759471EC88924D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16V → A in AAR82882 (PubMed:14742567).Curated1
Sequence conflicti18A → E in AAR99164 (Ref. 1) Curated1
Sequence conflicti18A → E in AAR82882 (PubMed:14742567).Curated1
Sequence conflicti31E → D in AAR99164 (Ref. 1) Curated1
Sequence conflicti109K → N in AAR99164 (Ref. 1) Curated1
Sequence conflicti109K → N in AAR82882 (PubMed:14742567).Curated1
Sequence conflicti128L → F in AAR99164 (Ref. 1) Curated1
Sequence conflicti128L → F in AAR82882 (PubMed:14742567).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF167344 Genomic DNA. Translation: AAR99164.1.
AY422197 Genomic DNA. Translation: AAR82882.1.
AL111168 Genomic DNA. Translation: CAL35264.1.
PIRiG81319.
RefSeqiWP_002858021.1. NC_002163.1.
YP_002344540.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL35264; CAL35264; Cj1149c.
GeneIDi905439.
KEGGicje:Cj1149c.
PATRICi20059254. VBICamJej33762_1130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF167344 Genomic DNA. Translation: AAR99164.1.
AY422197 Genomic DNA. Translation: AAR82882.1.
AL111168 Genomic DNA. Translation: CAL35264.1.
PIRiG81319.
RefSeqiWP_002858021.1. NC_002163.1.
YP_002344540.1. NC_002163.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TK9X-ray2.10A/B/C/D2-186[»]
ProteinModelPortaliQ9PNE6.
SMRiQ9PNE6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9PNE6. 103 interactors.
STRINGi192222.Cj1149c.

Proteomic databases

PaxDbiQ9PNE6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL35264; CAL35264; Cj1149c.
GeneIDi905439.
KEGGicje:Cj1149c.
PATRICi20059254. VBICamJej33762_1130.

Phylogenomic databases

eggNOGiENOG4105F55. Bacteria.
COG0279. LUCA.
HOGENOMiHOG000237571.
KOiK03271.
OMAiEMHILMI.

Enzyme and pathway databases

UniPathwayiUPA00041; UER00436.
UPA00976.
BioCyciCJEJ192222:GJTS-1076-MONOMER.
BRENDAi5.3.1.28. 1087.

Miscellaneous databases

EvolutionaryTraceiQ9PNE6.

Family and domain databases

HAMAPiMF_00067. GmhA. 1 hit.
InterProiIPR004515. Phosphoheptose_Isoase.
IPR001347. SIS.
[Graphical view]
PfamiPF13580. SIS_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00441. gmhA. 1 hit.
PROSITEiPS51464. SIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGMHA1_CAMJE
AccessioniPrimary (citable) accession number: Q9PNE6
Secondary accession number(s): Q0P9A7, Q6TG10, Q7BPS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The reaction produces a racemic mixture of D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate.By similarity

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.