Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoheptose isomerase 1

Gene

gmhA1

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate.By similarity

Catalytic activityi

D-sedoheptulose 7-phosphate = D-glycero-D-manno-heptose 7-phosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: D-glycero-D-manno-heptose 7-phosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Phosphoheptose isomerase 1 (gmhA1), Phosphoheptose isomerase 2 (gmhA2)
This subpathway is part of the pathway D-glycero-D-manno-heptose 7-phosphate biosynthesis, which is itself part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate, the pathway D-glycero-D-manno-heptose 7-phosphate biosynthesis and in Carbohydrate biosynthesis.

Pathwayi: LOS core biosynthesis

This protein is involved in the pathway LOS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LOS core biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi57 – 571ZincBy similarity
Metal bindingi61 – 611ZincBy similarity
Binding sitei61 – 611SubstrateBy similarity
Binding sitei121 – 1211SubstrateBy similarity
Metal bindingi168 – 1681ZincBy similarity
Binding sitei168 – 1681SubstrateBy similarity
Metal bindingi176 – 1761ZincBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-1120-MONOMER.
BRENDAi5.3.1.28. 1087.
UniPathwayiUPA00041; UER00436.
UPA00976.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoheptose isomerase 1 (EC:5.3.1.28)
Alternative name(s):
Sedoheptulose 7-phosphate isomerase 1
Gene namesi
Name:gmhA1
Synonyms:gmhA
Ordered Locus Names:Cj1149c
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 186186Phosphoheptose isomerase 1PRO_0000136522Add
BLAST

Proteomic databases

PaxDbiQ9PNE6.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiQ9PNE6. 103 interactions.
STRINGi192222.Cj1149c.

Structurei

Secondary structure

1
186
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 1817Combined sources
Helixi19 – 224Combined sources
Helixi23 – 3816Combined sources
Beta strandi43 – 486Combined sources
Helixi50 – 6314Combined sources
Beta strandi66 – 683Combined sources
Beta strandi75 – 795Combined sources
Helixi82 – 9110Combined sources
Helixi94 – 963Combined sources
Helixi97 – 1059Combined sources
Beta strandi111 – 1155Combined sources
Beta strandi117 – 1193Combined sources
Helixi122 – 13312Combined sources
Beta strandi137 – 1437Combined sources
Helixi144 – 1485Combined sources
Helixi149 – 1524Combined sources
Beta strandi154 – 1607Combined sources
Helixi164 – 18522Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TK9X-ray2.10A/B/C/D2-186[»]
ProteinModelPortaliQ9PNE6.
SMRiQ9PNE6. Positions 1-186.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9PNE6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 186154SISAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni48 – 503Substrate bindingBy similarity
Regioni90 – 912Substrate bindingBy similarity
Regioni116 – 1183Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the SIS family. GmhA subfamily.Curated
Contains 1 SIS domain.Curated

Phylogenomic databases

eggNOGiENOG4105F55. Bacteria.
COG0279. LUCA.
HOGENOMiHOG000237571.
KOiK03271.
OMAiEMHILMI.

Family and domain databases

HAMAPiMF_00067. GmhA. 1 hit.
InterProiIPR004515. Phosphoheptose_Isoase.
IPR001347. SIS.
[Graphical view]
PfamiPF13580. SIS_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00441. gmhA. 1 hit.
PROSITEiPS51464. SIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PNE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINLVEKEWQ EHQKIVQASE ILKGQIAKVG ELLCECLKKG GKILICGNGG
60 70 80 90 100
SAADAQHFAA ELSGRYKKER KALAGIALTT DTSALSAIGN DYGFEFVFSR
110 120 130 140 150
QVEALGNEKD VLIGISTSGK SPNVLEALKK AKELNMLCLG LSGKGGGMMN
160 170 180
KLCDHNLVVP SDDTARIQEM HILIIHTLCQ IIDESF
Length:186
Mass (Da):20,085
Last modified:October 1, 2000 - v1
Checksum:iD7759471EC88924D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161V → A in AAR82882 (PubMed:14742567).Curated
Sequence conflicti18 – 181A → E in AAR99164 (Ref. 1) Curated
Sequence conflicti18 – 181A → E in AAR82882 (PubMed:14742567).Curated
Sequence conflicti31 – 311E → D in AAR99164 (Ref. 1) Curated
Sequence conflicti109 – 1091K → N in AAR99164 (Ref. 1) Curated
Sequence conflicti109 – 1091K → N in AAR82882 (PubMed:14742567).Curated
Sequence conflicti128 – 1281L → F in AAR99164 (Ref. 1) Curated
Sequence conflicti128 – 1281L → F in AAR82882 (PubMed:14742567).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF167344 Genomic DNA. Translation: AAR99164.1.
AY422197 Genomic DNA. Translation: AAR82882.1.
AL111168 Genomic DNA. Translation: CAL35264.1.
PIRiG81319.
RefSeqiWP_002858021.1. NC_002163.1.
YP_002344540.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL35264; CAL35264; Cj1149c.
GeneIDi905439.
KEGGicje:Cj1149c.
PATRICi20059254. VBICamJej33762_1130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF167344 Genomic DNA. Translation: AAR99164.1.
AY422197 Genomic DNA. Translation: AAR82882.1.
AL111168 Genomic DNA. Translation: CAL35264.1.
PIRiG81319.
RefSeqiWP_002858021.1. NC_002163.1.
YP_002344540.1. NC_002163.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TK9X-ray2.10A/B/C/D2-186[»]
ProteinModelPortaliQ9PNE6.
SMRiQ9PNE6. Positions 1-186.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9PNE6. 103 interactions.
STRINGi192222.Cj1149c.

Proteomic databases

PaxDbiQ9PNE6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL35264; CAL35264; Cj1149c.
GeneIDi905439.
KEGGicje:Cj1149c.
PATRICi20059254. VBICamJej33762_1130.

Phylogenomic databases

eggNOGiENOG4105F55. Bacteria.
COG0279. LUCA.
HOGENOMiHOG000237571.
KOiK03271.
OMAiEMHILMI.

Enzyme and pathway databases

UniPathwayiUPA00041; UER00436.
UPA00976.
BioCyciCJEJ192222:GJTS-1120-MONOMER.
BRENDAi5.3.1.28. 1087.

Miscellaneous databases

EvolutionaryTraceiQ9PNE6.

Family and domain databases

HAMAPiMF_00067. GmhA. 1 hit.
InterProiIPR004515. Phosphoheptose_Isoase.
IPR001347. SIS.
[Graphical view]
PfamiPF13580. SIS_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00441. gmhA. 1 hit.
PROSITEiPS51464. SIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGMHA1_CAMJE
AccessioniPrimary (citable) accession number: Q9PNE6
Secondary accession number(s): Q0P9A7, Q6TG10, Q7BPS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The reaction produces a racemic mixture of D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate.By similarity

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.