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Q9PN58

- PDXA_CAMJE

UniProt

Q9PN58 - PDXA_CAMJE

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Protein

4-hydroxythreonine-4-phosphate dehydrogenase

Gene
pdxA, Cj1239
Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP) By similarity.UniRule annotation

Catalytic activityi

4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.UniRule annotation

Cofactori

Binds 1 divalent metal cation per subunit. Can use ions such as zinc, magnesium or cobalt By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei148 – 1481Substrate By similarity
Binding sitei149 – 1491Substrate By similarity
Metal bindingi177 – 1771Divalent metal cation; shared with dimeric partner By similarity
Metal bindingi216 – 2161Divalent metal cation; shared with dimeric partner By similarity
Metal bindingi301 – 3011Divalent metal cation; shared with dimeric partner By similarity
Binding sitei309 – 3091Substrate By similarity
Binding sitei318 – 3181Substrate By similarity
Binding sitei327 – 3271Substrate By similarity

GO - Molecular functioni

  1. 4-hydroxythreonine-4-phosphate dehydrogenase activity Source: UniProtKB-HAMAP
  2. cobalt ion binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. NAD binding Source: InterPro
  5. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. pyridoxal phosphate biosynthetic process Source: UniProtKB-HAMAP
  2. pyridoxine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyridoxine biosynthesis

Keywords - Ligandi

Cobalt, Magnesium, Metal-binding, NAD, NADP, Zinc

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-1211-MONOMER.
UniPathwayiUPA00244; UER00312.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxythreonine-4-phosphate dehydrogenase (EC:1.1.1.262)
Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenase
Gene namesi
Name:pdxA
Ordered Locus Names:Cj1239
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000000799: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3643644-hydroxythreonine-4-phosphate dehydrogenaseUniRule annotationPRO_0000188802Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

IntActiQ9PN58. 2 interactions.
STRINGi192222.Cj1239.

Structurei

Secondary structure

1
364
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 75
Turni11 – 144
Helixi15 – 2612
Turni27 – 293
Beta strandi30 – 356
Helixi38 – 4811
Beta strandi54 – 629
Beta strandi67 – 737
Beta strandi75 – 8410
Helixi103 – 12119
Beta strandi124 – 1307
Helixi135 – 1406
Helixi148 – 1569
Beta strandi161 – 1666
Beta strandi169 – 1757
Helixi180 – 1823
Helixi183 – 1864
Helixi189 – 20315
Beta strandi206 – 2116
Helixi215 – 2184
Turni219 – 2224
Helixi226 – 24419
Helixi248 – 2547
Helixi258 – 2669
Helixi281 – 2844
Helixi287 – 2926
Beta strandi295 – 3006
Helixi301 – 31111
Turni313 – 3153
Beta strandi317 – 3259
Beta strandi327 – 3293
Helixi348 – 35912

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TSNX-ray2.63A/B/C/D1-364[»]
ProteinModelPortaliQ9PN58.

Family & Domainsi

Sequence similaritiesi

Belongs to the PdxA family.

Phylogenomic databases

eggNOGiCOG1995.
HOGENOMiHOG000221591.
KOiK00097.
OMAiISIKLAM.
OrthoDBiEOG6GN6ZC.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_00536. PdxA.
InterProiIPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view]
PfamiPF04166. PdxA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PN58-1 [UniParc]FASTAAdd to Basket

« Hide

MKKLAISIGD INSIGLEILV RSHEELSKIC TPFYFIHESL LNKALKLLNL    50
KLFNAKIVAF KDDKDYEFNF IKKENSLEIY SFCLPLGFKV DENFEIQAGE 100
IDAKSGLYGF LSFKAASYFV YEKHAHALLT LPIHKKAWED AGLKYKGHTD 150
ALRDFFKKNA IMMLGCKELF VGLFSEHIPL AKVSKKITFK NLSIFLKDFY 200
KETHFKKMGL LGFNPHAGDY GVIGGEEEKI MEKAIAFVNA FLHSKKDEKF 250
FKKALKDENL QKELLLNFKG KGVYLPYPLV ADTAFTKTGL KNCNRLVAMY 300
HDLALAPLKA LYFDKSINVS LNLPIIRVSV DHGTAFDKAY KNAKINTKSY 350
FEAAKFAINL HSKA 364
Length:364
Mass (Da):41,387
Last modified:October 1, 2000 - v1
Checksum:iDDD26465821FFC3E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL111168 Genomic DNA. Translation: CAL35354.1.
PIRiA81331.
RefSeqiYP_002344630.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL35354; CAL35354; Cj1239.
GeneIDi905530.
KEGGicje:Cj1239.
PATRICi20059438. VBICamJej33762_1221.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL111168 Genomic DNA. Translation: CAL35354.1 .
PIRi A81331.
RefSeqi YP_002344630.1. NC_002163.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3TSN X-ray 2.63 A/B/C/D 1-364 [» ]
ProteinModelPortali Q9PN58.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9PN58. 2 interactions.
STRINGi 192222.Cj1239.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL35354 ; CAL35354 ; Cj1239 .
GeneIDi 905530.
KEGGi cje:Cj1239.
PATRICi 20059438. VBICamJej33762_1221.

Phylogenomic databases

eggNOGi COG1995.
HOGENOMi HOG000221591.
KOi K00097.
OMAi ISIKLAM.
OrthoDBi EOG6GN6ZC.

Enzyme and pathway databases

UniPathwayi UPA00244 ; UER00312 .
BioCyci CJEJ192222:GJTS-1211-MONOMER.

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
HAMAPi MF_00536. PdxA.
InterProi IPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view ]
Pfami PF04166. PdxA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 11168.

Entry informationi

Entry nameiPDXA_CAMJE
AccessioniPrimary (citable) accession number: Q9PN58
Secondary accession number(s): Q0P917
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The active site is located at the dimer interface By similarity.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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