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Q9PN47 (PUR2_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:Cj1250
OrganismCampylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168) [Reference proteome] [HAMAP]
Taxonomic identifier192222 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Protein attributes

Sequence length416 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

flaGQ0PAW91EBI-1192088,EBI-1191365

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 416416Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151439

Regions

Domain105 – 310206ATP-grasp
Nucleotide binding131 – 19262ATP By similarity

Sites

Metal binding2811Magnesium or manganese By similarity
Metal binding2831Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9PN47 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: E700C38A97AFE9D6

FASTA41645,316
        10         20         30         40         50         60 
MKIMILGSGA REYSIALALR RVDKNLEFYF APGNGATESL GTNLNLKDPV VLATYAKEKG 

        70         80         90        100        110        120 
FDLCIVGSES FLAEGVVDIF KQQGLAIFGP SKAAAMLETS KSFMKSFLKK YRIKTAKFLN 

       130        140        150        160        170        180 
TNDIEKAKNF IYSLTPPIVV KADGLCAGKG VIIAKTHEEA IEETAKMLSG ESFGDAGKLV 

       190        200        210        220        230        240 
VIEEFLDGYE LSIFAVCDGN DFVLLPAAQD HKKLLDNDQG PNTGGMGAYA PSSLANESLL 

       250        260        270        280        290        300 
RKVQKDIILP TLAGMKKEGA EFCGVLFIGA MIVGNKPYVL EFNVRFGDPE CEVLMPLIED 

       310        320        330        340        350        360 
PLELILAATQ RRLRHSKIKI KKEFAVGVVC ASENYPYKSS PKSEITVNNI PENSHISYAG 

       370        380        390        400        410 
VSLEDGKLMA DGGRVLVCVG TGKSIEEAQK NAYKLCDNVN FKGKQYRKDI AHQVLK 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL111168 Genomic DNA. Translation: CAL35365.1.
PIRD81332.
RefSeqYP_002344641.1. NC_002163.1.

3D structure databases

ProteinModelPortalQ9PN47.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9PN47. 23 interactions.
MINTMINT-6484378.
STRING192222.Cj1250.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL35365; CAL35365; Cj1250.
GeneID905541.
KEGGcje:Cj1250.
PATRIC20059462. VBICamJej33762_1233.

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMADPEAMNV.
OrthoDBEOG69SKD1.
ProtClustDBPRK00885.

Enzyme and pathway databases

BioCycCJEJ192222:GJTS-1222-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_CAMJE
AccessionPrimary (citable) accession number: Q9PN47
Secondary accession number(s): Q0P906
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: October 1, 2000
Last modified: February 19, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways