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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathway:iN(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei123 – 1231S-adenosyl-L-methionineUniRule annotation
Binding sitei148 – 1481S-adenosyl-L-methionineUniRule annotation
Binding sitei175 – 1751S-adenosyl-L-methionineUniRule annotation
Binding sitei201 – 2011SubstrateUniRule annotation
Binding sitei231 – 2311SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-1250-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Cj1278c
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000000799 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392tRNA (guanine-N(7)-)-methyltransferasePRO_0000171312Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi192222.Cj1278c.

Structurei

3D structure databases

ProteinModelPortaliQ9PN20.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000252163.
KOiK03439.
OMAiHFPVPWN.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PN20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNFKSKKIK EINLPYSKDD VEFLWLAKND NVSLIYTKVQ EESFFLQIKK
60 70 80 90 100
AQNGFVIKGD KHTKPSKIGY LQKALKIFKE GFCEDIINEA FGLKNNALIE
110 120 130 140 150
KTPFIVDNFD ELLSRLQGKI YIEIGFGSGR HLLYQAKENP NVLILGVEIY
160 170 180 190 200
NPALTQVAKL AKAQNVNNIL LIQSDARLLL SVLKSKSVEK IFLHFPVPWD
210 220 230 240 250
KKPHRRVIGK DFCKECARVL VQNGRFELRT DSFEYFNFTL EQFLTFPAPK
260 270 280 290 300
FSLRKNENLE ISSKYEDRWK KQEKNIYDLW VWNFNQECKN YELNEFNLSS
310 320 330 340 350
VEFSKEDLKK IEQNFKNITI KKDDFFLHFE SIYKQDENLL LKVAFGAFNK
360 370 380 390
PEHCYLHLDK TIDFAFKEPF KIQENIKAIN ELKEILKVQF KI
Length:392
Mass (Da):46,184
Last modified:October 1, 2000 - v1
Checksum:i1EA67D8B6EA4A190
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL35393.1.
PIRiH81335.
RefSeqiWP_002858203.1. NC_002163.1.
YP_002344669.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL35393; CAL35393; Cj1278c.
GeneIDi905569.
KEGGicje:Cj1278c.
PATRICi20059518. VBICamJej33762_1261.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL35393.1.
PIRiH81335.
RefSeqiWP_002858203.1. NC_002163.1.
YP_002344669.1. NC_002163.1.

3D structure databases

ProteinModelPortaliQ9PN20.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi192222.Cj1278c.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL35393; CAL35393; Cj1278c.
GeneIDi905569.
KEGGicje:Cj1278c.
PATRICi20059518. VBICamJej33762_1261.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000252163.
KOiK03439.
OMAiHFPVPWN.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciCJEJ192222:GJTS-1250-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 11168.

Entry informationi

Entry nameiTRMB_CAMJE
AccessioniPrimary (citable) accession number: Q9PN20
Secondary accession number(s): Q0P8X8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: October 1, 2000
Last modified: July 22, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.