Reviewed, UniProtKB/Swiss-Prot Q9PN20 (TRMB_CAMJE)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.33
Alternative name(s):
    tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	Cj1278c

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine. HAMAP MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Molecular function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	tRNA (guanine-N(7)-)-methyltransferase HAMAP MF_01057		PRO_0000171312
Sites
	Binding site	123	1	S-adenosyl-L-methionine By similarity
	Binding site	148	1	S-adenosyl-L-methionine By similarity
	Binding site	175	1	S-adenosyl-L-methionine By similarity
	Binding site	231	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9PN20-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 1EA67D8B6EA4A190	FASTA	392	46,184
	10 20 30 40 50 60 MPNFKSKKIK EINLPYSKDD VEFLWLAKND NVSLIYTKVQ EESFFLQIKK AQNGFVIKGD 70 80 90 100 110 120 KHTKPSKIGY LQKALKIFKE GFCEDIINEA FGLKNNALIE KTPFIVDNFD ELLSRLQGKI 130 140 150 160 170 180 YIEIGFGSGR HLLYQAKENP NVLILGVEIY NPALTQVAKL AKAQNVNNIL LIQSDARLLL 190 200 210 220 230 240 SVLKSKSVEK IFLHFPVPWD KKPHRRVIGK DFCKECARVL VQNGRFELRT DSFEYFNFTL 250 260 270 280 290 300 EQFLTFPAPK FSLRKNENLE ISSKYEDRWK KQEKNIYDLW VWNFNQECKN YELNEFNLSS 310 320 330 340 350 360 VEFSKEDLKK IEQNFKNITI KKDDFFLHFE SIYKQDENLL LKVAFGAFNK PEHCYLHLDK 370 380 390 TIDFAFKEPF KIQENIKAIN ELKEILKVQF KI

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL111168 Genomic DNA. Translation: CAL35393.1.
PIR	H81335.
RefSeq	YP_002344669.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	905569.
GenomeReviews	Gene locus Cj1278c in contig AL111168_GR.
KEGG	cje:Cj1278c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q9PN20. FVHFPVP.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ1278C-MON.
BRENDA	2.1.1.33. 255835.
Family and domain databases
HAMAP	MF_01057. [Tree]
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
PANTHER	PTHR23417:SF1. Methyltransf_4. 1 hit.
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. CHP91. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_CAMJE

Accession

Primary (citable) accession number: Q9PN20
Secondary accession number(s): Q0P8X8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)