Q9PMS6 (NTPA_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

Cj1374c

Organism

Campylobacter jejuni

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
dcd	Q9PN07	3	EBI-1271444,EBI-1271615

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178145
Regions
	Region	7 – 12	6	Substrate binding By similarity
	Region	73 – 74	2	Substrate binding By similarity
Sites
	Metal binding	38	1	Magnesium or manganese By similarity
	Metal binding	73	1	Magnesium or manganese By similarity
	Binding site	157	1	Substrate By similarity
	Binding site	177	1	Substrate By similarity
	Binding site	183	1	Substrate By similarity
Experimental info
	Sequence conflict	120	1	Y → H in AAD10059. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9PMS6 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 02F29AF6FEB0FBA1	FASTA	200	22,375
	10 20 30 40 50 60 MKIILATSNK HKVLELKEIL KDFEIYAFDE VLMPFEIEEN GKTFKENALI KARAVFNALD 70 80 90 100 110 120 EKQKKDFIAL SDDSGICVDV LEGNPGIYSA RFSGKGDDKS NRDKLVNEMI KKGFKQSKAY 130 140 150 160 170 180 YVAAIAMVGL MGEFSTHGTM HGKVIDTEKG ENGFGYDSLF IPKGFDKTLA QLSVDEKNNI 190 200 SHRFKALELA KIILKILNKG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a multidrug-efflux transporter homolog from Campylobacter jejuni." Gilbert M., Michniewicz J., Wakarchuk W.W. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: OH4384 / Serotype O:19.
[2]	"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF113952 Genomic DNA. Translation: AAD10059.1. AL111168 Genomic DNA. Translation: CAL35486.1.
PIR	C81282.
RefSeq	YP_002344762.1. NC_002163.1.
3D structure databases
ProteinModelPortal	Q9PMS6.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PMS6. 41 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	905667.
GenomeReviews	Gene locus Cj1374c in contig AL111168_GR.
KEGG	cje:Cj1374c.
PATRIC	20059713. VBICamJej33762_1355.
Phylogenomic databases
HOGENOM	HBG697237.
OMA	GVYTADW.
ProtClustDB	PRK00120.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ1374C-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_CAMJE

Accession

Primary (citable) accession number: Q9PMS6
Secondary accession number(s): Q0P8N5, Q9ZF65

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program