Reviewed, UniProtKB/Swiss-Prot Q9PMG8 (CARA_CAMJE)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	Cj1494c

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112264
Regions
	Domain	188 – 372	185	Glutamine amidotransferase type-1
	Region	1 – 184	184	CPSase HAMAP MF_01209
Sites
	Active site	268	1	Nucleophile By similarity
	Active site	351	1	By similarity
	Active site	353	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PMG8-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 151D54A6769AE04D	FASTA	372	42,095
	10 20 30 40 50 60 MKAYIYLEND IFLSAKAFGK SGTFFGELVF NTSLTGYQEI ISDPSYAGQF IVFSMPEIGI 70 80 90 100 110 120 VGTNENDNES KEIFASGVLM RELSSSFSNF RAKESLQDYL EKHGKIGIYE LDTRYLVKMI 130 140 150 160 170 180 RNNGNLRAVI STEISNKEDL KIALEKSAKI DEVNFVKEVS TKKNYSHKQG VWNASFQKFN 190 200 210 220 230 240 DAKRSEKKVA VIDYGVKTNI LNELVEVGFE VEVYPYNVKA DELITLYKKG EIQGVFLSNG 250 260 270 280 290 300 PGEPRILKQE IAEIKKLAEA KIPMLGICLG HQLLSNAFGY ETYKMKFGQH GANHPVINLD 310 320 330 340 350 360 TKTVEITAQN HNYNVPEELA QVAHITHRNL FGDNVEGVRY KDYPIISVQH HPESSSGPHE 370 SKYIFKEFMN LM

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL111168 Genomic DNA. Translation: CAL35601.1.
PIR	C81296.
RefSeq	YP_002344874.1.
3D structure databases
HSSP	HSSP built from PDB template 1M6V based on UniProtKB P00907.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PMG8. 29 interactions.
Genome annotation databases
GeneID	905785.
GenomeReviews	Gene locus Cj1494c in contig AL111168_GR.
KEGG	cje:Cj1494c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q9PMG8. EADIPFF.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ1494C-MON.
BRENDA	6.3.5.5. 255835.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_CAMJE

Accession

Primary (citable) accession number: Q9PMG8
Secondary accession number(s): Q0P8C3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)