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Protein

Histidine biosynthesis bifunctional protein HisIE

Gene

hisI

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-AMP + diphosphate.
1-(5-phospho-beta-D-ribosyl)-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide.

Pathwayi: L-histidine biosynthesis

This protein is involved in step 2 and 3 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (hisG)
  2. Histidine biosynthesis bifunctional protein HisIE (hisI)
  3. Histidine biosynthesis bifunctional protein HisIE (hisI)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
  5. Imidazole glycerol phosphate synthase subunit hisF1 (hisF1), Imidazole glycerol phosphate synthase subunit HisH 2 (hisH2), Imidazole glycerol phosphate synthase subunit HisH 1 (hisH1), Putative imidazole glycerol phosphate synthase subunit hisF2 (hisF2)
  6. Histidine biosynthesis bifunctional protein HisB (hisB)
  7. Histidinol-phosphate aminotransferase (hisC)
  8. Histidine biosynthesis bifunctional protein HisB (hisB)
  9. Histidinol dehydrogenase (hisD)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAmino-acid biosynthesis, Histidine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-1505-MONOMER.
UniPathwayiUPA00031; UER00007.
UPA00031; UER00008.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine biosynthesis bifunctional protein HisIE
Including the following 2 domains:
Phosphoribosyl-AMP cyclohydrolase (EC:3.5.4.19)
Short name:
PRA-CH
Phosphoribosyl-ATP pyrophosphatase (EC:3.6.1.31)
Short name:
PRA-PH
Gene namesi
Name:hisI
Synonyms:hisIE
Ordered Locus Names:Cj1604
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001364081 – 207Histidine biosynthesis bifunctional protein HisIEAdd BLAST207

Proteomic databases

PaxDbiQ9PM71.

Interactioni

Protein-protein interaction databases

IntActiQ9PM71. 7 interactors.
STRINGi192222.Cj1604.

Structurei

3D structure databases

ProteinModelPortaliQ9PM71.
SMRiQ9PM71.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 117Phosphoribosyl-AMP cyclohydrolaseAdd BLAST117
Regioni118 – 207Phosphoribosyl-ATP pyrophosphohydrolaseAdd BLAST90

Sequence similaritiesi

In the N-terminal section; belongs to the PRA-CH family.Curated
In the C-terminal section; belongs to the PRA-PH family.Curated

Phylogenomic databases

eggNOGiENOG4105K8F. Bacteria.
COG0139. LUCA.
COG0140. LUCA.
HOGENOMiHOG000277503.
KOiK11755.
OMAiCHTGNKT.

Family and domain databases

CDDicd11534. NTP-PPase_HisIE_like. 1 hit.
HAMAPiMF_01020. HisE. 1 hit.
MF_01019. HisIE. 1 hit.
InterProiView protein in InterPro
IPR023019. His_synth_HisIE.
IPR008179. HisE.
IPR021130. PRib-ATP_PPHydrolase-like.
IPR002496. PRib_AMP_CycHydrolase_dom.
PfamiView protein in Pfam
PF01502. PRA-CH. 1 hit.
PF01503. PRA-PH. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002610. PRA_CycHdrlase. 1 hit.
TIGRFAMsiTIGR03188. histidine_hisI. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9PM71-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNFKELNEK IAWQKVDNLL PVIIQDAKTC EVLMLGFMNN EALEKSLESG
60 70 80 90 100
KVVFFSRTKQ RLWMKGEESG NFLNIIDLSL DCDNDTLLIL ANPVGPTCHT
110 120 130 140 150
GDISCFEKIS KNADFVFLAR LEKLINARKN ADENTSYTAK LFKSGTKRIA
160 170 180 190 200
QKVGEEGVET ALAATVKDKE ELICEAADLM YHLSVLLADA NLSFSDVISK

LKERHKA
Length:207
Mass (Da):23,176
Last modified:October 1, 2000 - v1
Checksum:iF5E4E6374D6E99C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL35701.1.
PIRiB81256.
RefSeqiWP_002851143.1. NC_002163.1.
YP_002344973.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL35701; CAL35701; Cj1604.
GeneIDi905159.
KEGGicje:Cj1604.
PATRICi20060168. VBICamJej33762_1580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL35701.1.
PIRiB81256.
RefSeqiWP_002851143.1. NC_002163.1.
YP_002344973.1. NC_002163.1.

3D structure databases

ProteinModelPortaliQ9PM71.
SMRiQ9PM71.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9PM71. 7 interactors.
STRINGi192222.Cj1604.

Proteomic databases

PaxDbiQ9PM71.

Protocols and materials databases

DNASUi905159.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL35701; CAL35701; Cj1604.
GeneIDi905159.
KEGGicje:Cj1604.
PATRICi20060168. VBICamJej33762_1580.

Phylogenomic databases

eggNOGiENOG4105K8F. Bacteria.
COG0139. LUCA.
COG0140. LUCA.
HOGENOMiHOG000277503.
KOiK11755.
OMAiCHTGNKT.

Enzyme and pathway databases

UniPathwayiUPA00031; UER00007.
UPA00031; UER00008.
BioCyciCJEJ192222:GJTS-1505-MONOMER.

Family and domain databases

CDDicd11534. NTP-PPase_HisIE_like. 1 hit.
HAMAPiMF_01020. HisE. 1 hit.
MF_01019. HisIE. 1 hit.
InterProiView protein in InterPro
IPR023019. His_synth_HisIE.
IPR008179. HisE.
IPR021130. PRib-ATP_PPHydrolase-like.
IPR002496. PRib_AMP_CycHydrolase_dom.
PfamiView protein in Pfam
PF01502. PRA-CH. 1 hit.
PF01503. PRA-PH. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002610. PRA_CycHdrlase. 1 hit.
TIGRFAMsiTIGR03188. histidine_hisI. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHIS2_CAMJE
AccessioniPrimary (citable) accession number: Q9PM71
Secondary accession number(s): Q0P824
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: October 1, 2000
Last modified: March 15, 2017
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.