Reviewed, UniProtKB/Swiss-Prot Q9PM41 (AROC_CAMJE)

Names and origin

Protein names

Recommended name:
    Chorismate synthase
    EC=4.2.3.5
Alternative name(s):
    5-enolpyruvylshikimate-3-phosphate phospholyase

Gene names

Name:	aroC
Ordered Locus Names:	Cj1634c

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate. HAMAP MF_00300
Cofactor	Reduced flavin By similarity.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 7/7. HAMAP MF_00300
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the chorismate synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Molecular function	Lyase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	chorismate synthase activity Inferred from electronic annotation. Source: HAMAP protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
flgE2	Q0P7Q2	1	EBI-1329003,EBI-1193196
flgK	Q0P8E9	1	EBI-1329003,EBI-1191246
flhA	Q0PA15	1	EBI-1329003,EBI-1193184
fliE	Q9PHY8	1	EBI-1329003,EBI-1191580

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 362

362

Chorismate synthase HAMAP MF_00300

PRO_0000140568

Secondary structure

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362

Helix Strand Turn

Details...

Beta strand

6 – 13

8

Helix

16 – 18

3

Beta strand

21 – 26

6

Helix

36 – 45

10

Beta strand

62 – 64

3

Beta strand

66 – 69

4

Beta strand

78 – 82

5

Helix

128 – 143

16

Turn

144 – 146

3

Beta strand

148 – 156

9

Helix

165 – 167

3

Helix

170 – 175

6

Turn

183 – 185

3

Helix

186 – 198

13

Beta strand

205 – 213

9

Turn

221 – 223

3

Helix

226 – 235

10

Beta strand

240 – 245

6

Helix

248 – 253

6

Helix

256 – 259

4

Beta strand

264 – 267

4

Beta strand

275 – 277

3

Beta strand

280 – 284

5

Beta strand

286 – 292

7

Helix

325 – 346

22

Helix

348 – 350

3

Helix

352 – 358

7

Sequences

	Sequence		Length	Mass (Da)
	Q9PM41-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 7A9EDAE23F59AA75	FASTA	362	39,252
	10 20 30 40 50 60 MNTFGTRLKF TSFGESHGVA VGCIIDGMPA GVKFDEEFLQ NELDKRKGGS KFATPRKESD 70 80 90 100 110 120 KAQVLSGVFE GYTTGHPIAI VVFNENAHSK DYDNLKDLFR PAHADFTYFY KYGIRDHRGG 130 140 150 160 170 180 GRSSARESVA RVAGGAVAAM LLREFDICVQ SGVFGVGTFV SNLKEEEFDF EFAKKSEIFC 190 200 210 220 230 240 LDPKLESDFK NEILNARNSK DSVGAAVFTK VSGMLIGLGE VLYDKLDSKL AHALMGINAV 250 260 270 280 290 300 KAVEIGEGIN ASKMRGSCNN DALKDGKFLS NHSGGILGGI SNGENLILKT YFKPTPSIFA 310 320 330 340 350 360 KQESIDKFGN NLKFELKGRH DPCVGVRGSV VASAMVRLVL ADCLLLNASA NLNNLKNAYG LK

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AL111168 Genomic DNA. Translation: CAL35731.1.

PIR

H81259.

RefSeq

YP_002345003.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SQ1	X-ray	2.80	A	1-362	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9PM41. 94 interactions.

Genome annotation databases

GeneID

905907.

GenomeReviews

Gene locus Cj1634c in contig AL111168_GR.

KEGG

cje:Cj1634c.

Organism-specific databases

CMR

Search...

Phylogenomic databases

OMA

Q9PM41. SRFTTQR.

Enzyme and pathway databases

BioCyc

CJEJ192222:CJ1634C-MON.

BRENDA

4.2.3.5. 255835.

Family and domain databases

HAMAP

MF_00300.
[Tree]

InterPro

IPR000453. Chorismate_synth.
[Graphical view]

PANTHER

PTHR21085. Chorismate_synth. 1 hit.

Pfam

PF01264. Chorismate_synt. 1 hit.
[Graphical view]

PIRSF

PIRSF001456. Chorismate_synth. 1 hit.

ProDom

PD002941. Chorismate_synth. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00033. aroC. 1 hit.

PROSITE

PS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
PS00789. CHORISMATE_SYNTHASE_3. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

AROC_CAMJE

Accession

Primary (citable) accession number: Q9PM41
Secondary accession number(s): Q0P7Z4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)