Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribonuclease 3

Gene

rnc

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon (By similarity).By similarity
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In this organism endogenous ribonuclease 3 and Cas9 are required for correct coprocessing of pre-crRNA and the trans-encoded small RNA (tracrRNA). Cas9, crRNA and tracrRNA are required for cleavage of invading DNA (Probable). Complements pre-crRNA and tracrRNA coprocessing defects in an rnc deletion in S.pyogenes strain 370 (PubMed:24270795).Curated1 Publication

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40MagnesiumUniRule annotation1
Active sitei44UniRule annotation1
Metal bindingi113MagnesiumUniRule annotation1
Active sitei116UniRule annotation1
Metal bindingi116MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease, RNA-binding, rRNA-binding
Biological processmRNA processing, rRNA processing, tRNA processing
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciCJEJ192222:G1G1F-1591-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3UniRule annotation (EC:3.1.26.3UniRule annotation)
Alternative name(s):
Ribonuclease IIIUniRule annotation
Short name:
RNase IIIUniRule annotation
Gene namesi
Name:rncUniRule annotation
Ordered Locus Names:Cj1635c
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001803831 – 224Ribonuclease 3Add BLAST224

Proteomic databases

PaxDbiQ9PM40
PRIDEiQ9PM40

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiQ9PM40, 5 interactors
STRINGi192222.Cj1635c

Structurei

Secondary structure

1224
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 11Combined sources8
Helixi18 – 24Combined sources7
Helixi37 – 58Combined sources22
Turni59 – 61Combined sources3
Helixi67 – 75Combined sources9
Helixi77 – 86Combined sources10
Helixi89 – 91Combined sources3
Helixi97 – 101Combined sources5
Helixi104 – 106Combined sources3
Helixi108 – 126Combined sources19
Helixi128 – 142Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3N3WX-ray2.21A/B1-224[»]
3O2RX-ray1.25A/B/C/D1-146[»]
ProteinModelPortaliQ9PM40
SMRiQ9PM40
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 127RNase IIIUniRule annotationAdd BLAST124
Domaini154 – 223DRBMUniRule annotationAdd BLAST70

Sequence similaritiesi

Belongs to the ribonuclease III family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108ZBM Bacteria
COG0571 LUCA
HOGENOMiHOG000246808
KOiK03685
OMAiLTHKSCK

Family and domain databases

CDDicd00593 RIBOc, 1 hit
Gene3Di1.10.1520.10, 1 hit
HAMAPiMF_00104 RNase_III, 1 hit
InterProiView protein in InterPro
IPR014720 dsRBD_dom
IPR011907 RNase_III
IPR000999 RNase_III_dom
IPR036389 RNase_III_sf
PfamiView protein in Pfam
PF00035 dsrm, 1 hit
PF14622 Ribonucleas_3_3, 1 hit
SMARTiView protein in SMART
SM00358 DSRM, 1 hit
SM00535 RIBOc, 1 hit
SUPFAMiSSF69065 SSF69065, 1 hit
TIGRFAMsiTIGR02191 RNaseIII, 1 hit
PROSITEiView protein in PROSITE
PS50137 DS_RBD, 1 hit
PS00517 RNASE_3_1, 1 hit
PS50142 RNASE_3_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9PM40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNIEKLEQS LTYEFKDKNL LIHALTHKSF KKSYNNERLE FLGDAVLDLV
60 70 80 90 100
VGEYLFHKFA KDAEGDLSKL RAALVNEKSF AKIANSLNLG DFILMSVAEE
110 120 130 140 150
NNGGKEKPSI LSDALEAIIG AIHLEAGFEF AKTIALRLIE KNFPQIDAKI
160 170 180 190 200
LIKDYKTKLQ EITQGKIGQT PQYETVRAFG PDHLKQFEIA LMLDGKELAR
210 220
AIAGSKKEAQ QMAAKIALEK LGAL
Length:224
Mass (Da):24,964
Last modified:October 1, 2000 - v1
Checksum:i4AB69FD394DB17CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA Translation: CAL35732.1
PIRiA81260
RefSeqiWP_002851153.1, NC_002163.1
YP_002345004.1, NC_002163.1

Genome annotation databases

EnsemblBacteriaiCAL35732; CAL35732; Cj1635c
GeneIDi905908
KEGGicje:Cj1635c
PATRICifig|192222.6.peg.1611

Similar proteinsi

Entry informationi

Entry nameiRNC_CAMJE
AccessioniPrimary (citable) accession number: Q9PM40
Secondary accession number(s): Q0P7Z3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health