Reviewed, UniProtKB/Swiss-Prot Q9PM01 (MURB_CAMJE)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	Cj1676

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murB family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000179190
Sites
	Active site	142	1	By similarity
	Active site	184	1	Proton donor By similarity
	Active site	254	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PM01-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 151A42905129C2C6	FASTA	258	28,994
	10 20 30 40 50 60 MIIDFKKYSS VRIGNEFEVL VLDQICDFDG FLIGGANNLL VSPKPKNIGI LGDGFNFIQI 70 80 90 100 110 120 LDRNKDFIHL RIGCKTKSSK MYRFAKENNL KGFEYLSKIP GTLGGLLKMN AGLKGECISQ 130 140 150 160 170 180 NLIKIATSQG EILRANINFD YRFCPLNTHF FWAEFKLNFG FDTLKDEALK NARSNQPSGA 190 200 210 220 230 240 SFGSIFKNPK NDFAGRLIEA VGLKGFSKGD AMLSDKHANF LINKKNASFE DAFFLIELAR 250 KKVFEEFGTN LENEVIII

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL111168 Genomic DNA. Translation: CAL35772.1.
PIR	A81265.
RefSeq	YP_002345044.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PM01. 8 interactions.
Genome annotation databases
GeneID	905951.
GenomeReviews	Gene locus Cj1676 in contig AL111168_GR.
KEGG	cje:Cj1676.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LVKMNAG.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ1676-MON.
BRENDA	1.1.1.158. 255835.
Family and domain databases
HAMAP	MF_00037. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR003170. MurB. IPR011601. MurB_C. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURB_CAMJE

Accession

Primary (citable) accession number: Q9PM01
Secondary accession number(s): Q0P7V3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)