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Q9PLZ2

- TOP1_CAMJE

UniProt

Q9PLZ2 - TOP1_CAMJE

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Protein
DNA topoisomerase 1
Gene
topA, Cj1686c
Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Magnesium 1; catalytic By similarity
Sitei33 – 331Interaction with DNA By similarity
Metal bindingi83 – 831Magnesium 1; catalytic By similarity
Metal bindingi83 – 831Magnesium 2 By similarity
Metal bindingi85 – 851Magnesium 2 By similarity
Sitei140 – 1401Interaction with DNA By similarity
Sitei141 – 1411Interaction with DNA By similarity
Sitei144 – 1441Interaction with DNA By similarity
Sitei149 – 1491Interaction with DNA By similarity
Active sitei298 – 2981O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei300 – 3001Interaction with DNA By similarity
Sitei485 – 4851Interaction with DNA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri573 – 59927C4-type 1UniRule annotation
Add
BLAST
Zinc fingeri629 – 65628C4-type 2UniRule annotation
Add
BLAST
Zinc fingeri669 – 69224C4-type 3UniRule annotation
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-1652-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topA
Ordered Locus Names:Cj1686c
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000000799: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 700700DNA topoisomerase 1UniRule annotation
PRO_0000145146Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi192222.Cj1686c.

Structurei

3D structure databases

ProteinModelPortaliQ9PLZ2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 114112Toprim
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni164 – 1696Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0551.
HOGENOMiHOG000004018.
KOiK03168.
OMAiVVECDKC.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PLZ2-1 [UniParc]FASTAAdd to Basket

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MKKNLIIVES PAKAKTIGNF LGKDYEVIAS KGHIRDLPKS SFGIKIEDDE    50
FIPEYRITSD HSALVKELKS KAKDAKEVYL ATDEDREGEA IAYHIAKAIG 100
KDENTLPRIV FHEITKSAIE NALKNPRKLN MHSVNAQQTR RLLDRIVGYK 150
LSPLLGQKIQ RGLSAGRVQS AALKIIVDRE KEIRAFVPLE YFSIDMIFQK 200
DLDAELVEFD KAKIEKLTIT NKDRAKLILE ACKNEVYSIS DIESKERKIA 250
PPPPFMTSTL QQSASNRLGF NPKKTMMIAQ KLYEGVNTHE GVMGVITYMR 300
TDSLNLAKEA VENARKFIQA NFGKDYLPSK ANVYTTKTKG AQEAHEAIRP 350
TNLSFTPEIA AKFLDKDELK LYTLIYNRFL ACQMSPAISQ TQNVFVKNDR 400
VVFKISGRKI LFDGYYKVYG DMDKDKILPN FKIGQNLKVQ NLEMNSHFTE 450
PPSRYSEAGL VKKLESLGIG RPSTYAPTIS ILTSRDYVTI DKKQLIPSDV 500
AFNVTEVLEK NFSDIVDSKF TSNLENTLDE IAEDKADWQE TLKEFYYPFM 550
RKIEEGKTKI ASQKTVTKLG ESCPDCGGEL AIRKGRFGEF VACLNFPKCK 600
YSRNLKSESK NESENTAAKA KANGTGITCP SCQKGEIVER FSKRGKFYGC 650
SAYPKCNFIS KYKPSEEKCE ECGETLVIKE LKKGTFLECL KCKIKKEMKD 700
Length:700
Mass (Da):79,148
Last modified:October 1, 2000 - v1
Checksum:i86CE6DE6A8231A00
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL111168 Genomic DNA. Translation: CAL35780.1.
PIRiB81266.
RefSeqiYP_002345052.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL35780; CAL35780; Cj1686c.
GeneIDi905960.
KEGGicje:Cj1686c.
PATRICi20060346. VBICamJej33762_1660.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL111168 Genomic DNA. Translation: CAL35780.1 .
PIRi B81266.
RefSeqi YP_002345052.1. NC_002163.1.

3D structure databases

ProteinModelPortali Q9PLZ2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 192222.Cj1686c.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL35780 ; CAL35780 ; Cj1686c .
GeneIDi 905960.
KEGGi cje:Cj1686c.
PATRICi 20060346. VBICamJej33762_1660.

Phylogenomic databases

eggNOGi COG0551.
HOGENOMi HOG000004018.
KOi K03168.
OMAi VVECDKC.
OrthoDBi EOG6S7XQ9.

Enzyme and pathway databases

BioCyci CJEJ192222:GJTS-1652-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 11168.

Entry informationi

Entry nameiTOP1_CAMJE
AccessioniPrimary (citable) accession number: Q9PLZ2
Secondary accession number(s): Q0P7U5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: October 1, 2000
Last modified: September 3, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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