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Protein

DNA topoisomerase 1

Gene

topA

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9Magnesium 1; catalyticUniRule annotation1
Metal bindingi83Magnesium 1; catalyticUniRule annotation1
Metal bindingi83Magnesium 2UniRule annotation1
Metal bindingi85Magnesium 2UniRule annotation1
Active sitei298O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri573 – 599C4-type 1Add BLAST27
Zinc fingeri629 – 656C4-type 2Add BLAST28
Zinc fingeri669 – 692C4-type 3Add BLAST24

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-1592-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:Cj1686c
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001451461 – 700DNA topoisomerase 1Add BLAST700

Proteomic databases

PaxDbiQ9PLZ2.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei33Interaction with DNAUniRule annotation1
Sitei140Interaction with DNAUniRule annotation1
Sitei141Interaction with DNAUniRule annotation1
Sitei144Interaction with DNAUniRule annotation1
Sitei149Interaction with DNAUniRule annotation1
Sitei300Interaction with DNAUniRule annotation1
Sitei485Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRINGi192222.Cj1686c.

Structurei

3D structure databases

ProteinModelPortaliQ9PLZ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 114ToprimUniRule annotationAdd BLAST112

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni164 – 169Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri573 – 599C4-type 1Add BLAST27
Zinc fingeri629 – 656C4-type 2Add BLAST28
Zinc fingeri669 – 692C4-type 3Add BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG4105C73. Bacteria.
COG0550. LUCA.
COG0551. LUCA.
HOGENOMiHOG000004018.
KOiK03168.
OMAiVVECDKC.

Family and domain databases

CDDicd00186. TOP1Ac. 1 hit.
Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 3 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PLZ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKNLIIVES PAKAKTIGNF LGKDYEVIAS KGHIRDLPKS SFGIKIEDDE
60 70 80 90 100
FIPEYRITSD HSALVKELKS KAKDAKEVYL ATDEDREGEA IAYHIAKAIG
110 120 130 140 150
KDENTLPRIV FHEITKSAIE NALKNPRKLN MHSVNAQQTR RLLDRIVGYK
160 170 180 190 200
LSPLLGQKIQ RGLSAGRVQS AALKIIVDRE KEIRAFVPLE YFSIDMIFQK
210 220 230 240 250
DLDAELVEFD KAKIEKLTIT NKDRAKLILE ACKNEVYSIS DIESKERKIA
260 270 280 290 300
PPPPFMTSTL QQSASNRLGF NPKKTMMIAQ KLYEGVNTHE GVMGVITYMR
310 320 330 340 350
TDSLNLAKEA VENARKFIQA NFGKDYLPSK ANVYTTKTKG AQEAHEAIRP
360 370 380 390 400
TNLSFTPEIA AKFLDKDELK LYTLIYNRFL ACQMSPAISQ TQNVFVKNDR
410 420 430 440 450
VVFKISGRKI LFDGYYKVYG DMDKDKILPN FKIGQNLKVQ NLEMNSHFTE
460 470 480 490 500
PPSRYSEAGL VKKLESLGIG RPSTYAPTIS ILTSRDYVTI DKKQLIPSDV
510 520 530 540 550
AFNVTEVLEK NFSDIVDSKF TSNLENTLDE IAEDKADWQE TLKEFYYPFM
560 570 580 590 600
RKIEEGKTKI ASQKTVTKLG ESCPDCGGEL AIRKGRFGEF VACLNFPKCK
610 620 630 640 650
YSRNLKSESK NESENTAAKA KANGTGITCP SCQKGEIVER FSKRGKFYGC
660 670 680 690 700
SAYPKCNFIS KYKPSEEKCE ECGETLVIKE LKKGTFLECL KCKIKKEMKD
Length:700
Mass (Da):79,148
Last modified:October 1, 2000 - v1
Checksum:i86CE6DE6A8231A00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL35780.1.
PIRiB81266.
RefSeqiWP_002851268.1. NC_002163.1.
YP_002345052.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL35780; CAL35780; Cj1686c.
GeneIDi905960.
KEGGicje:Cj1686c.
PATRICi20060346. VBICamJej33762_1660.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL35780.1.
PIRiB81266.
RefSeqiWP_002851268.1. NC_002163.1.
YP_002345052.1. NC_002163.1.

3D structure databases

ProteinModelPortaliQ9PLZ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi192222.Cj1686c.

Proteomic databases

PaxDbiQ9PLZ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL35780; CAL35780; Cj1686c.
GeneIDi905960.
KEGGicje:Cj1686c.
PATRICi20060346. VBICamJej33762_1660.

Phylogenomic databases

eggNOGiENOG4105C73. Bacteria.
COG0550. LUCA.
COG0551. LUCA.
HOGENOMiHOG000004018.
KOiK03168.
OMAiVVECDKC.

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-1592-MONOMER.

Family and domain databases

CDDicd00186. TOP1Ac. 1 hit.
Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 3 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOP1_CAMJE
AccessioniPrimary (citable) accession number: Q9PLZ2
Secondary accession number(s): Q0P7U5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.