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Protein

DNA topoisomerase 1

Gene

topA

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9Magnesium 1; catalyticUniRule annotation1
Metal bindingi83Magnesium 1; catalyticUniRule annotation1
Metal bindingi83Magnesium 2UniRule annotation1
Metal bindingi85Magnesium 2UniRule annotation1
Active sitei298O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri573 – 599C4-type 1Add BLAST27
Zinc fingeri629 – 656C4-type 2Add BLAST28
Zinc fingeri669 – 692C4-type 3Add BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciCJEJ192222:G1G1F-1645-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:Cj1686c
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001451461 – 700DNA topoisomerase 1Add BLAST700

Proteomic databases

PaxDbiQ9PLZ2
PRIDEiQ9PLZ2

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei33Interaction with DNAUniRule annotation1
Sitei140Interaction with DNAUniRule annotation1
Sitei141Interaction with DNAUniRule annotation1
Sitei144Interaction with DNAUniRule annotation1
Sitei149Interaction with DNAUniRule annotation1
Sitei300Interaction with DNAUniRule annotation1
Sitei485Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRINGi192222.Cj1686c

Structurei

3D structure databases

ProteinModelPortaliQ9PLZ2
SMRiQ9PLZ2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 114ToprimUniRule annotationAdd BLAST112

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni164 – 169Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri573 – 599C4-type 1Add BLAST27
Zinc fingeri629 – 656C4-type 2Add BLAST28
Zinc fingeri669 – 692C4-type 3Add BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG4105C73 Bacteria
COG0550 LUCA
COG0551 LUCA
HOGENOMiHOG000004018
KOiK03168
OMAiPECKYTR

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03363 TOPRIM_TopoIA_TopoI, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 2 hits
2.70.20.10, 2 hits
HAMAPiMF_00952 Topoisom_1_prok, 1 hit
InterProiView protein in InterPro
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR013498 Topo_IA_Znf
IPR005733 TopoI_bac-type
IPR028612 Topoisom_1_IA
IPR006171 TOPRIM_domain
IPR034149 TOPRIM_TopoI
PfamiView protein in Pfam
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PF01396 zf-C4_Topoisom, 3 hits
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 1 hit
TIGRFAMsiTIGR01051 topA_bact, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

Q9PLZ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKNLIIVES PAKAKTIGNF LGKDYEVIAS KGHIRDLPKS SFGIKIEDDE
60 70 80 90 100
FIPEYRITSD HSALVKELKS KAKDAKEVYL ATDEDREGEA IAYHIAKAIG
110 120 130 140 150
KDENTLPRIV FHEITKSAIE NALKNPRKLN MHSVNAQQTR RLLDRIVGYK
160 170 180 190 200
LSPLLGQKIQ RGLSAGRVQS AALKIIVDRE KEIRAFVPLE YFSIDMIFQK
210 220 230 240 250
DLDAELVEFD KAKIEKLTIT NKDRAKLILE ACKNEVYSIS DIESKERKIA
260 270 280 290 300
PPPPFMTSTL QQSASNRLGF NPKKTMMIAQ KLYEGVNTHE GVMGVITYMR
310 320 330 340 350
TDSLNLAKEA VENARKFIQA NFGKDYLPSK ANVYTTKTKG AQEAHEAIRP
360 370 380 390 400
TNLSFTPEIA AKFLDKDELK LYTLIYNRFL ACQMSPAISQ TQNVFVKNDR
410 420 430 440 450
VVFKISGRKI LFDGYYKVYG DMDKDKILPN FKIGQNLKVQ NLEMNSHFTE
460 470 480 490 500
PPSRYSEAGL VKKLESLGIG RPSTYAPTIS ILTSRDYVTI DKKQLIPSDV
510 520 530 540 550
AFNVTEVLEK NFSDIVDSKF TSNLENTLDE IAEDKADWQE TLKEFYYPFM
560 570 580 590 600
RKIEEGKTKI ASQKTVTKLG ESCPDCGGEL AIRKGRFGEF VACLNFPKCK
610 620 630 640 650
YSRNLKSESK NESENTAAKA KANGTGITCP SCQKGEIVER FSKRGKFYGC
660 670 680 690 700
SAYPKCNFIS KYKPSEEKCE ECGETLVIKE LKKGTFLECL KCKIKKEMKD
Length:700
Mass (Da):79,148
Last modified:October 1, 2000 - v1
Checksum:i86CE6DE6A8231A00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA Translation: CAL35780.1
PIRiB81266
RefSeqiWP_002851268.1, NC_002163.1
YP_002345052.1, NC_002163.1

Genome annotation databases

EnsemblBacteriaiCAL35780; CAL35780; Cj1686c
GeneIDi905960
KEGGicje:Cj1686c
PATRICifig|192222.6.peg.1660

Similar proteinsi

Entry informationi

Entry nameiTOP1_CAMJE
AccessioniPrimary (citable) accession number: Q9PLZ2
Secondary accession number(s): Q0P7U5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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