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Q9PLW0 (LEU3_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3-isopropylmalate dehydrogenase
EC=1.1.1.85
Alternative name(s):
3-IPM-DH
Beta-IPM dehydrogenase
Short name=IMDH
Gene names
Name:leuB
Ordered Locus Names:Cj1718c
OrganismCampylobacter jejuni
Taxonomic identifier197 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length358 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. HAMAP MF_01033

Catalytic activity

(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH. HAMAP MF_01033

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. HAMAP MF_01033

Subunit structure

Homodimer By similarity. HAMAP MF_01033

Subcellular location

Cytoplasm By similarity HAMAP MF_01033.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3583583-isopropylmalate dehydrogenase HAMAP MF_01033
PRO_0000083675

Regions

Nucleotide binding77 – 9014NAD By similarity
Nucleotide binding279 – 29113NAD By similarity

Sites

Metal binding2211Magnesium or manganese By similarity
Metal binding2451Magnesium or manganese By similarity
Metal binding2491Magnesium or manganese By similarity
Binding site981Substrate By similarity
Binding site1081Substrate By similarity
Binding site1371Substrate By similarity
Binding site2211Substrate By similarity
Site1441Important for catalysis By similarity
Site1891Important for catalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9PLW0 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 26B811645E7FFA83

FASTA35839,368
        10         20         30         40         50         60 
MKTYKVAVLA GDGIGPLVMK EALKILTFIA QKYNFSFEFN EAKIGGASID AYGVALSDET 

        70         80         90        100        110        120 
LKLCEQSDAI LFGSVGGPKW DNLPIDQRPE RASLLPLRKH FNLFANLRPC KIYESLTHAS 

       130        140        150        160        170        180 
PLKNEIIQKG VDILCVRELT GGIYFGKQDL GKESAYDTEI YTKKEIERIA RIAFESARIR 

       190        200        210        220        230        240 
KKKVHLIDKA NVLASSILWR EVVANVAKDY QDINLEYMYV DNAAMQIVKN PSIFDVMLCS 

       250        260        270        280        290        300 
NLFGDILSDE LAAINGSLGL LSSASLNDKG FGLYEPAGGS APDIAHLNIA NPIAQILSAA 

       310        320        330        340        350 
LMLKYSFKEE QAAQDIENAI SLALAQGKMT KDLNAKSYLN TDEMGDCILE ILKENDNG

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL111168 Genomic DNA. Translation: CAL35812.1.
PIRB81270.
RefSeqYP_002345084.1. NC_002163.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3UDOX-ray2.30A1-358[»]
3UDUX-ray1.85A/B/C/D/E/F/G/H1-358[»]
ProteinModelPortalQ9PLW0.
SMRQ9PLW0. Positions 2-354.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9PLW0. 6 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID905995.
GenomeReviewsGene locus Cj1718c in contig AL111168_GR.
KEGGcje:Cj1718c.
PATRIC20060418. VBICamJej33762_1692.

Phylogenomic databases

HOGENOMHBG518924.
OMAFESARIR.
ProtClustDBPRK00772.

Enzyme and pathway databases

BioCycCJEJ192222:CJ1718C-MONOMER.

Family and domain databases

HAMAPMF_01033. LeuB_type1.
[Tree]
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
IPR004429. Isopropylmalate_DH.
[Graphical view]
Gene3DG3DSA:3.40.718.10. IDH_IMDH. 1 hit.
KOK00052.
PANTHERPTHR11835. IDH_IMDH_dimeric. 1 hit.
PTHR11835:SF13. IPMDH. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsTIGR00169. LeuB. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEU3_CAMJE
AccessionPrimary (citable) accession number: Q9PLW0
Secondary accession number(s): Q0P7R3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 1, 2000
Last modified: January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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