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Protein

3-isopropylmalate dehydrogenase

Gene

leuB

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.

Catalytic activityi

(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 Mg2+ or Mn2+ ion per subunit.UniRule annotation

Pathwayi: L-leucine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase (leuA)
  2. 3-isopropylmalate dehydratase small subunit (leuD), 3-isopropylmalate dehydratase large subunit (leuC)
  3. 3-isopropylmalate dehydrogenase (leuB)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei98SubstrateUniRule annotation1
Binding sitei108SubstrateUniRule annotation1
Binding sitei137SubstrateUniRule annotation1
Sitei144Important for catalysisUniRule annotation1
Sitei189Important for catalysisUniRule annotation1
Metal bindingi221Magnesium or manganeseUniRule annotation1
Binding sitei221SubstrateUniRule annotation1
Metal bindingi245Magnesium or manganeseUniRule annotation1
Metal bindingi249Magnesium or manganeseUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 90NADUniRule annotationAdd BLAST14
Nucleotide bindingi279 – 291NADUniRule annotationAdd BLAST13

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-1628-MONOMER.
UniPathwayiUPA00048; UER00072.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydrogenaseUniRule annotation (EC:1.1.1.85UniRule annotation)
Alternative name(s):
3-IPM-DHUniRule annotation
Beta-IPM dehydrogenaseUniRule annotation
Short name:
IMDHUniRule annotation
Gene namesi
Name:leuBUniRule annotation
Ordered Locus Names:Cj1718c
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000836751 – 3583-isopropylmalate dehydrogenaseAdd BLAST358

Proteomic databases

PaxDbiQ9PLW0.
PRIDEiQ9PLW0.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiQ9PLW0. 6 interactors.
STRINGi192222.Cj1718c.

Structurei

Secondary structure

1358
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 12Combined sources10
Helixi15 – 33Combined sources19
Beta strandi36 – 41Combined sources6
Helixi46 – 52Combined sources7
Beta strandi53 – 56Combined sources4
Helixi58 – 65Combined sources8
Beta strandi67 – 74Combined sources8
Helixi78 – 80Combined sources3
Helixi85 – 87Combined sources3
Helixi89 – 101Combined sources13
Beta strandi105 – 111Combined sources7
Helixi114 – 119Combined sources6
Beta strandi120 – 122Combined sources3
Helixi124 – 127Combined sources4
Beta strandi132 – 138Combined sources7
Helixi142 – 144Combined sources3
Beta strandi148 – 150Combined sources3
Beta strandi152 – 162Combined sources11
Helixi163 – 179Combined sources17
Beta strandi182 – 188Combined sources7
Turni190 – 192Combined sources3
Helixi194 – 206Combined sources13
Helixi207 – 209Combined sources3
Beta strandi213 – 219Combined sources7
Helixi220 – 229Combined sources10
Helixi231 – 233Combined sources3
Beta strandi235 – 239Combined sources5
Helixi241 – 255Combined sources15
Helixi258 – 260Combined sources3
Beta strandi262 – 266Combined sources5
Beta strandi268 – 270Combined sources3
Beta strandi272 – 278Combined sources7
Helixi282 – 284Combined sources3
Helixi293 – 305Combined sources13
Helixi310 – 325Combined sources16
Turni331 – 333Combined sources3
Beta strandi335 – 337Combined sources3
Helixi341 – 354Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UDOX-ray2.30A1-358[»]
3UDUX-ray1.85A/B/C/D/E/F/G/H1-358[»]
ProteinModelPortaliQ9PLW0.
SMRiQ9PLW0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0C. Bacteria.
COG0473. LUCA.
HOGENOMiHOG000021112.
KOiK00052.
OMAiRRPKQFD.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_01033. LeuB_type1. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
IPR004429. Isopropylmalate_DH.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00169. leuB. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PLW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTYKVAVLA GDGIGPLVMK EALKILTFIA QKYNFSFEFN EAKIGGASID
60 70 80 90 100
AYGVALSDET LKLCEQSDAI LFGSVGGPKW DNLPIDQRPE RASLLPLRKH
110 120 130 140 150
FNLFANLRPC KIYESLTHAS PLKNEIIQKG VDILCVRELT GGIYFGKQDL
160 170 180 190 200
GKESAYDTEI YTKKEIERIA RIAFESARIR KKKVHLIDKA NVLASSILWR
210 220 230 240 250
EVVANVAKDY QDINLEYMYV DNAAMQIVKN PSIFDVMLCS NLFGDILSDE
260 270 280 290 300
LAAINGSLGL LSSASLNDKG FGLYEPAGGS APDIAHLNIA NPIAQILSAA
310 320 330 340 350
LMLKYSFKEE QAAQDIENAI SLALAQGKMT KDLNAKSYLN TDEMGDCILE

ILKENDNG
Length:358
Mass (Da):39,368
Last modified:October 1, 2000 - v1
Checksum:i26B811645E7FFA83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL35812.1.
PIRiB81270.
RefSeqiWP_002858793.1. NC_002163.1.
YP_002345084.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL35812; CAL35812; Cj1718c.
GeneIDi905995.
KEGGicje:Cj1718c.
PATRICi20060418. VBICamJej33762_1692.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL35812.1.
PIRiB81270.
RefSeqiWP_002858793.1. NC_002163.1.
YP_002345084.1. NC_002163.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UDOX-ray2.30A1-358[»]
3UDUX-ray1.85A/B/C/D/E/F/G/H1-358[»]
ProteinModelPortaliQ9PLW0.
SMRiQ9PLW0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9PLW0. 6 interactors.
STRINGi192222.Cj1718c.

Proteomic databases

PaxDbiQ9PLW0.
PRIDEiQ9PLW0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL35812; CAL35812; Cj1718c.
GeneIDi905995.
KEGGicje:Cj1718c.
PATRICi20060418. VBICamJej33762_1692.

Phylogenomic databases

eggNOGiENOG4105C0C. Bacteria.
COG0473. LUCA.
HOGENOMiHOG000021112.
KOiK00052.
OMAiRRPKQFD.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00072.
BioCyciCJEJ192222:GJTS-1628-MONOMER.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_01033. LeuB_type1. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
IPR004429. Isopropylmalate_DH.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00169. leuB. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEU3_CAMJE
AccessioniPrimary (citable) accession number: Q9PLW0
Secondary accession number(s): Q0P7R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.