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Q9PLT3 (Q9PLT3_CHLMU) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesSubmitted name:
DNA topoisomerase I EMBL AAF38905.1
Gene names
Name:topA EMBL AAF38905.1
Ordered Locus Names:TC_0012
OrganismChlamydia muridarum [Complete proteome] [HAMAP]
Taxonomic identifier83560 [NCBI]
Taxonomic lineageBacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length865 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733

Sequence similarities

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
Q9PLT3 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 4A3588647BC39755

FASTA86597,933
        10         20         30         40         50         60 
MKKSLIIVES PAKIKTLRKL LGEGFIFDSS LGHIVDLPAK GFGIDIEKGF IPDYQILEGK 

        70         80         90        100        110        120 
EEVIRKICAE AKKCDVVYLA PDPDREGEAI AWHIANQLPK NTKIQRISFN AITKGAVTEA 

       130        140        150        160        170        180 
LKHPREIDMA LVNAQQARRF LDRIVGYKIS PILGRKLQRW SGVSAGRVQS VALKLVVDRE 

       190        200        210        220        230        240 
YAIEQFVPVE FWNIRVNLQD PKSQKTFWAH LHSVNGKKWE KEIPEGKSSE DVVLIDSKEK 

       250        260        270        280        290        300 
ADDLVSLLES ATYCVDRVES KEKKRNAYPP FITSTLQQEA SRHYRFSSSR TMNIAQTLYE 

       310        320        330        340        350        360 
GVDLDSQGAV GLITYMRTDS VRTDPEAIKQ VRKYIENHFG KEYVPSSPNM YATKKMAQDA 

       370        380        390        400        410        420 
HEAIRPTDVS LSPESIRTKL TEDQYKLYSL IWKRFVASQM IAAIYDTLAI QIATNKGIDL 

       430        440        450        460        470        480 
RATGSCLKFK GFLAVYEEKR DEEGDEDENI QLPKLHERDE LKKEEIEAEQ SHTKPLPRFT 

       490        500        510        520        530        540 
EASLVKELEK SGIGRPSTYA TIMNKIQSRE YTLKEGQRLR PTELGKVVCQ FLETNFPRIM 

       550        560        570        580        590        600 
DIGFTAKMED ELELIADNKK PWKQLLQEFC ELFLPFVVTA EKEAFIPRIV TEMDCPRCHK 

       610        620        630        640        650        660 
GKLVKIWSKN RYFFGCSEYP TCDYKTSEEE LTFDKSEYAD DTPWDAPCAL CGGQMKVRHG 

       670        680        690        700        710        720 
KFGSFLGCEN YPQCHYIVNL FKKGEAGSEP EEIVSCPAEG CTGHLVKRRS RFNKMFYSCS 

       730        740        750        760        770        780 
EYPACSVIGN SVDAVIEKYT GTPKTPYEKK IKAKKATASK KGKATKGKAS TTKTKKKSTV 

       790        800        810        820        830        840 
TTKNRKTATY TPSSALAAVI GPDPIDGFPE ATKKIWAYIK EQGLQSPNNK RVIVPDSKMK 

       850        860 
HVIGDDPIDM FALSKKIQAH LTKQE

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE002160 Genomic DNA. Translation: AAF38905.1.
PIRH81749.
RefSeqNP_296396.1. NC_002620.2.

3D structure databases

HSSPHSSP built from PDB template 1ECL based on UniProtKB P06612.
ProteinModelPortalQ9PLT3.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1245536.
GenomeReviewsGene locus TC_0012 in contig AE002160_GR.
KEGGcmu:TC0012.
PATRIC20371360. VBIChlMur19118_0022.
TIGRTC_0012.

Phylogenomic databases

HOGENOMHBG512935.
OMAEEYWSID.
PhylomeDBQ9PLT3.
ProtClustDBPRK06319.

Family and domain databases

InterProIPR019835. SWIB_domain.
IPR003121. SWIB_MDM2_domain.
IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF02201. SWIB. 1 hit.
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 3 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00151. SWIB. 1 hit.
SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF47592. MDM2. 1 hit.
SSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ9PLT3_CHLMU
AccessionPrimary (citable) accession number: Q9PLT3
Entry history
Integrated into UniProtKB/TrEMBL: October 1, 2000
Last sequence update: October 1, 2000
Last modified: January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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