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Protein

Ribonucleoside-diphosphate reductase subunit alpha

Gene

nrdA

Organism
Chlamydia muridarum (strain MoPn / Nigg)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).By similarity

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei442SubstrateBy similarity1
Sitei458Important for hydrogen atom transferBy similarity1
Sitei465Allosteric effector bindingBy similarity1
Binding sitei486Substrate; via amide nitrogenBy similarity1
Sitei495Allosteric effector bindingBy similarity1
Active sitei670Proton acceptorBy similarity1
Active sitei672Cysteine radical intermediateBy similarity1
Active sitei674Proton acceptorBy similarity1
Sitei687Important for hydrogen atom transferBy similarity1
Sitei990Important for electron transferBy similarity1
Sitei991Important for electron transferBy similarity1
Sitei1043Interacts with thioredoxin/glutaredoxinBy similarity1
Sitei1046Interacts with thioredoxin/glutaredoxinBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase
Gene namesi
Name:nrdA
Ordered Locus Names:TC_0214
OrganismiChlamydia muridarum (strain MoPn / Nigg)
Taxonomic identifieri243161 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000000800 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872101 – 1047Ribonucleoside-diphosphate reductase subunit alphaAdd BLAST1047

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi458 ↔ 687Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ9PL93.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

STRINGi243161.CmurN_010100001068.

Structurei

3D structure databases

ProteinModelPortaliQ9PL93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 111ATP-cone 1PROSITE-ProRule annotationAdd BLAST103
Domaini118 – 219ATP-cone 2PROSITE-ProRule annotationAdd BLAST102
Domaini237 – 327ATP-cone 3PROSITE-ProRule annotationAdd BLAST91

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni457 – 458Substrate bindingBy similarity2
Regioni670 – 674Substrate bindingBy similarity5
Regioni857 – 861Substrate bindingBy similarity5

Sequence similaritiesi

Contains 3 ATP-cone domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105BZH. Bacteria.
COG0209. LUCA.
KOiK00525.
OMAiFDYLGLQ.

Family and domain databases

InterProiIPR005144. ATP-cone_dom.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 2 hits.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 3 hits.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PL93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDLQEKQCT IVKRNGMFVP FDRNRIFQAL EAAFRDTRRI DDHMPLPEDL
60 70 80 90 100
ENSIRSITHQ VVKEVVQKIT DGQVVTVERI QDMVESQLYI NGLQDVARDY
110 120 130 140 150
VVYRDDRKAH REKSWQSLSV IRRCGTTVHF NPMKISAALE KAFRATDRIE
160 170 180 190 200
GMTPDFVREE VNALTQKIVA EIEERCSQQD SRIDIEQIQD IVEQQLMVVG
210 220 230 240 250
HYATAKNYIL YREARARVRD NRVEDQIVEE APSEETFEVL SKDGSTYMIT
260 270 280 290 300
HSQLLARLAR ACSRFPETTD AALLTDMAFS NFYSGIKESE VVLACIMAAR
310 320 330 340 350
ANIEKEPDYA FVAAELLLDV VYKEALDRSR GDEDLEQVYR DHFKRYIMEG
360 370 380 390 400
DSYRLNPELK NLFDLDALAN AMDLSRDLQF SYMGIQNLYD RYFNHDDGRR
410 420 430 440 450
LETPQIFWMR VAMGLALKEQ DKTYWAITFY NLLSTFRYTP ATPTLFNSGM
460 470 480 490 500
RHSQLSSCYL STVQDDLVNI YKVISDNAML SKWAGGIGND WTAIRATGAL
510 520 530 540 550
IKGTNGKSQG VIPFIKVTND TAVAVNQGGK RKGAVCVYLE VWHLDYEDFL
560 570 580 590 600
ELRKNTGDDR RRAHDVNTAS WIPDLFFKRL QQKGSWTLFS PDDVPGLHDA
610 620 630 640 650
YGEEFERLYE EYERKVDSGE IRLYKKVEAE DLWRKMLSML FETGHPWMTF
660 670 680 690 700
KDPSNIRSAQ DHTGVVRCSN LCTEILLNCS ETETAVCNLG SVNLVQHILD
710 720 730 740 750
DGLDEEKLSE TISIAVRMLD NVIDINFYPT KEAKEANFAH RAIGLGVMGF
760 770 780 790 800
QDALYKLDIS YASQEAVEFA DYSSELISYY AIQASCLLAK ERGTYSSYKG
810 820 830 840 850
SKWDRGLLPI DTIQLLANYR GKDNLQMDTS VRKDWEPIRS LIREHGMRNC
860 870 880 890 900
QLMAIAPTAT ISNIIGVTQS IEPTYKHLFV KSNLSGEFTI PNVYLIEKLK
910 920 930 940 950
KLGIWDADML DDLKYFDGSL LEIERVPDHI KHIFLTAFEI EPEWILECAS
960 970 980 990 1000
RRQKWIDMGQ SLNLYLAQPD GKKLSNMYLT AWKKGLKTTY YLRSSSATTV
1010 1020 1030 1040
EKSFVDINKR GIQPRWMKNK SASAGIVVER ASKTPVCSLE EGCEVCQ
Length:1,047
Mass (Da):120,036
Last modified:October 1, 2000 - v1
Checksum:i9B3FFD9BFAF817AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE002160 Genomic DNA. Translation: AAF39086.1.
PIRiF81728.
RefSeqiWP_010229837.1. NZ_ACOV01000003.1.

Genome annotation databases

EnsemblBacteriaiAAF39086; AAF39086; TC_0214.
GeneIDi1246340.
KEGGicmu:TC_0214.
PATRICi20371810. VBIChlMur19118_0234.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE002160 Genomic DNA. Translation: AAF39086.1.
PIRiF81728.
RefSeqiWP_010229837.1. NZ_ACOV01000003.1.

3D structure databases

ProteinModelPortaliQ9PL93.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243161.CmurN_010100001068.

Proteomic databases

PRIDEiQ9PL93.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF39086; AAF39086; TC_0214.
GeneIDi1246340.
KEGGicmu:TC_0214.
PATRICi20371810. VBIChlMur19118_0234.

Phylogenomic databases

eggNOGiENOG4105BZH. Bacteria.
COG0209. LUCA.
KOiK00525.
OMAiFDYLGLQ.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

InterProiIPR005144. ATP-cone_dom.
IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF03477. ATP-cone. 2 hits.
PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS51161. ATP_CONE. 3 hits.
PS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIR1_CHLMU
AccessioniPrimary (citable) accession number: Q9PL93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.