Reviewed, UniProtKB/Swiss-Prot Q9PL93 (RIR1_CHLMU)

Names and origin

Protein names

Recommended name:
    Ribonucleoside-diphosphate reductase subunit alpha
    EC=1.17.4.1
Alternative name(s):
    Ribonucleotide reductase

Gene names

Name:	nrdA
Ordered Locus Names:	TC_0214

Organism

Chlamydia muridarum [Complete proteome] [HAMAP]

Taxonomic identifier

83560 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia

Protein attributes

Sequence length	1047 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase large chain family. Contains 3 ATP-cone domains.

Ontologies

Keywords
Biological process	DNA replication
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Oxidoreductase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro ribonucleoside-diphosphate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1047	1047	Ribonucleoside-diphosphate reductase subunit alpha		PRO_0000187210
Regions
	Domain	9 – 111	103	ATP-cone 1
	Domain	118 – 219	102	ATP-cone 2
	Domain	237 – 327	91	ATP-cone 3
Sites
	Active site	458	1	Hydrogen atom transfer By similarity
	Active site	670	1	Proton acceptor By similarity
	Active site	672	1	Proton acceptor By similarity
	Active site	674	1	Proton acceptor By similarity
	Active site	687	1	Hydrogen atom transfer By similarity
	Active site	990	1	Electron transfer By similarity
	Active site	991	1	Electron transfer By similarity
	Site	465	1	Allosteric effector binding By similarity
	Site	495	1	Allosteric effector binding By similarity
	Site	1043	1	Interacts with thioredoxin/glutaredoxin By similarity
	Site	1046	1	Interacts with thioredoxin/glutaredoxin By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PL93-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 9B3FFD9BFAF817AA	FASTA	1,047	120,036
	10 20 30 40 50 60 MVDLQEKQCT IVKRNGMFVP FDRNRIFQAL EAAFRDTRRI DDHMPLPEDL ENSIRSITHQ 70 80 90 100 110 120 VVKEVVQKIT DGQVVTVERI QDMVESQLYI NGLQDVARDY VVYRDDRKAH REKSWQSLSV 130 140 150 160 170 180 IRRCGTTVHF NPMKISAALE KAFRATDRIE GMTPDFVREE VNALTQKIVA EIEERCSQQD 190 200 210 220 230 240 SRIDIEQIQD IVEQQLMVVG HYATAKNYIL YREARARVRD NRVEDQIVEE APSEETFEVL 250 260 270 280 290 300 SKDGSTYMIT HSQLLARLAR ACSRFPETTD AALLTDMAFS NFYSGIKESE VVLACIMAAR 310 320 330 340 350 360 ANIEKEPDYA FVAAELLLDV VYKEALDRSR GDEDLEQVYR DHFKRYIMEG DSYRLNPELK 370 380 390 400 410 420 NLFDLDALAN AMDLSRDLQF SYMGIQNLYD RYFNHDDGRR LETPQIFWMR VAMGLALKEQ 430 440 450 460 470 480 DKTYWAITFY NLLSTFRYTP ATPTLFNSGM RHSQLSSCYL STVQDDLVNI YKVISDNAML 490 500 510 520 530 540 SKWAGGIGND WTAIRATGAL IKGTNGKSQG VIPFIKVTND TAVAVNQGGK RKGAVCVYLE 550 560 570 580 590 600 VWHLDYEDFL ELRKNTGDDR RRAHDVNTAS WIPDLFFKRL QQKGSWTLFS PDDVPGLHDA 610 620 630 640 650 660 YGEEFERLYE EYERKVDSGE IRLYKKVEAE DLWRKMLSML FETGHPWMTF KDPSNIRSAQ 670 680 690 700 710 720 DHTGVVRCSN LCTEILLNCS ETETAVCNLG SVNLVQHILD DGLDEEKLSE TISIAVRMLD 730 740 750 760 770 780 NVIDINFYPT KEAKEANFAH RAIGLGVMGF QDALYKLDIS YASQEAVEFA DYSSELISYY 790 800 810 820 830 840 AIQASCLLAK ERGTYSSYKG SKWDRGLLPI DTIQLLANYR GKDNLQMDTS VRKDWEPIRS 850 860 870 880 890 900 LIREHGMRNC QLMAIAPTAT ISNIIGVTQS IEPTYKHLFV KSNLSGEFTI PNVYLIEKLK 910 920 930 940 950 960 KLGIWDADML DDLKYFDGSL LEIERVPDHI KHIFLTAFEI EPEWILECAS RRQKWIDMGQ 970 980 990 1000 1010 1020 SLNLYLAQPD GKKLSNMYLT AWKKGLKTTY YLRSSSATTV EKSFVDINKR GIQPRWMKNK 1030 1040 SASAGIVVER ASKTPVCSLE EGCEVCQ

References

[1]

"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MoPn / Nigg.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE002160 Genomic DNA. Translation: AAF39086.1.
PIR	F81728.
RefSeq	NP_296593.1.
3D structure databases
HSSP	HSSP built from PDB template 3R1R based on UniProtKB P00452.
ModBase	Search...
Genome annotation databases
GeneID	1246340.
GenomeReviews	Gene locus TC_0214 in contig AE002160_GR.
KEGG	cmu:TC0214.
TIGR	TC_0214.
Phylogenomic databases
HOGENOM	Q9PL93.
OMA	Q9PL93. YNSKEGY.
Enzyme and pathway databases
BioCyc	CMUR243161:TC_0214-MON.
BRENDA	1.17.4.1. 256349.
Family and domain databases
InterPro	IPR005144. ATP-cone. IPR013346. NrdE_NrdA. IPR013509. Ribncl_Rdtase_lsu_N. IPR000788. Ribncl_red_lg_C. [Graphical view]
PANTHER	PTHR11573. Ribncl_red_lg_C. 1 hit.
Pfam	PF03477. ATP-cone. 3 hits. PF02867. Ribonuc_red_lgC. 1 hit. PF00317. Ribonuc_red_lgN. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
TIGRFAMs	TIGR02506. NrdE_NrdA. 1 hit.
PROSITE	PS51161. ATP_CONE. 3 hits. PS00089. RIBORED_LARGE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR1_CHLMU

Accession

Primary (citable) accession number: Q9PL93

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)