Reviewed, UniProtKB/Swiss-Prot Q9PL91 (TRMB_CHLMU)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.33
Alternative name(s):
    tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	TC_0216

Organism

Chlamydia muridarum [Complete proteome] [HAMAP]

Taxonomic identifier

83560 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine. HAMAP MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Molecular function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	tRNA (guanine-N(7)-)-methyltransferase HAMAP MF_01057		PRO_0000171315
Sites
	Binding site	54	1	S-adenosyl-L-methionine By similarity
	Binding site	79	1	S-adenosyl-L-methionine By similarity
	Binding site	106	1	S-adenosyl-L-methionine By similarity
	Binding site	129	1	S-adenosyl-L-methionine By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	165	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9PL91-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 8DC9A831A7B8A700	FASTA	224	26,516
	10 20 30 40 50 60 MKPQDLKLPY FWEDRCPKIE NHVFYVPSYY PKYEEFVMPS WQNLFANEGP VCCELCSGNG 70 80 90 100 110 120 DWVVEQALKD TSINWIAVEK RFDRVRKIWS KMSNHKVNNL LIVCGEAQTF FTHYVTDASF 130 140 150 160 170 180 QKIVVNFPDP WPKFRHRKHR LFQDVFVQDM VRVLVEGGQL TLVTDDHTYL TNSIQVMLNY 190 200 210 220 LSPGMQDPYY VNVKDNYGGS WFENLWRSKG EKIFCTEFVK RVGI

References

[1]

"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MoPn / Nigg.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE002160 Genomic DNA. Translation: AAF39088.1.
PIR	F81726.
RefSeq	NP_296595.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1246342.
GenomeReviews	Gene locus TC_0216 in contig AE002160_GR.
KEGG	cmu:TC0216.
TIGR	TC_0216.
Phylogenomic databases
HOGENOM	Q9PL91.
OMA	Q9PL91. INWIAVE.
Enzyme and pathway databases
BioCyc	CMUR243161:TC_0216-MON.
BRENDA	2.1.1.33. 256349.
Family and domain databases
HAMAP	MF_01057. [Tree]
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
PANTHER	PTHR23417:SF1. Methyltransf_4. 1 hit.
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMB_CHLMU

Accession

Primary (citable) accession number: Q9PL91

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)