Q9PL83 (SYFA_CHLMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	TC_0224

Organism

Chlamydia muridarum [Complete proteome] [HAMAP]

Taxonomic identifier

83560 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_0000126686
Sites
	Metal binding	256	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PL83 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 62426ADD147EFB89	FASTA	341	38,781
	10 20 30 40 50 60 MTIQEELEAV KQQFSCDLSL VHSSKDLFDL KVKYLGKKGI FRGFADQLRE CPVEQKATIG 70 80 90 100 110 120 ASINACKQYI EEVLLEKSQV ILAKEEAEEF LKEKVDVSLP GEDAPLGGKH IIKKVLDDVV 130 140 150 160 170 180 DIFVRFGFCV REAPNIESEK NNFSLLNFEE DHPARQMQDT FYLDPVTVLR THTSNVQSRE 190 200 210 220 230 240 LARNKPPVRV VAPGECFRNE DISARSHVTF HQVEAFHVDR DVSFSDLTSM LSGFYHIFFG 250 260 270 280 290 300 RKVELRYRHS YFPFVEPGIE VDISCECRGA GCSLCKHSGW LEVAGAGMIH PNVLRQANID 310 320 330 340 PEEYSGYALG MGIERLAMLK YGISDIRLFS ENDLRFLRQF S

References

[1]

"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MoPn / Nigg.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE002160 Genomic DNA. Translation: AAF39096.1.
PIR	F81727.
RefSeq	NP_296603.1. NC_002620.2.
3D structure databases
ProteinModelPortal	Q9PL83.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1246392.
GenomeReviews	Gene locus TC_0224 in contig AE002160_GR.
KEGG	cmu:TC0224.
PATRIC	20371832. VBIChlMur19118_0244.
TIGR	TC_0224.
Phylogenomic databases
HOGENOM	HBG284353.
OMA	FRASYFP.
PhylomeDB	Q9PL83.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	CMUR243161:TC_0224-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_CHLMU

Accession

Primary (citable) accession number: Q9PL83

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program