Reviewed, UniProtKB/Swiss-Prot Q9PL64 (FUMC_CHLMU)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II
      Short name=Fumarase C
    EC=4.2.1.2

Gene names

Name:	fumC
Ordered Locus Names:	TC_0244

Organism

Chlamydia muridarum [Complete proteome] [HAMAP]

Taxonomic identifier

83560 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_00743
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161267
Regions
	Region	126 – 129	4	B site By similarity
	Region	136 – 138	3	Substrate binding By similarity
Sites
	Binding site	97	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PL64-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 7F3C5EA2F2991332	FASTA	463	50,428
	10 20 30 40 50 60 MRQENDSLGI VMVPEDKLFG AQTGRSKNFF SYGKELMPIE VIQALVKIKK CAAKANGDLQ 70 80 90 100 110 120 CLDAKRRDMI VAASDEILSG GLEEHFPLKV WQTGSGTQSN MNVNEVIANL AIKRHGGELG 130 140 150 160 170 180 SKNPVHPNDH VNKSQSSNDV FPTAMHIAAV QSIKGSLIPA LEHLQKNLDA KALEFSRDIK 190 200 210 220 230 240 IGRTHLMDAV PMTLGQEFSG YSHQLRSCLE RIGFSLTHLY ELAIGGTAVG TGLNVPEGFV 250 260 270 280 290 300 DKVIYYLRQE TGEPFIPASN YFAALSNHDA LVQAHGSLAV LACSLIKIAT DLSFLGSGPR 310 320 330 340 350 360 CGLGEIFFPE NEPGSSIMPG KINPTQSEAL QMVCSQVIGN NQSVIFSGTK GNFELNVMKP 370 380 390 400 410 420 VIIYDFLQSV NLLSGAMRSF ADYFVSGLKV NRGQLQQNVE RSLMLVTALA PVLGYDKCSK 430 440 450 460 IALKAFHENL SLKEACVSLG FLSEQEFDTH VVPGLMLGKR ERE

References

[1]

"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MoPn / Nigg.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE002160 Genomic DNA. Translation: AAF39114.1.
PIR	B81725.
RefSeq	NP_296623.1.
3D structure databases
HSSP	HSSP built from PDB template 1YFM based on UniProtKB P08417.
ModBase	Search...
Genome annotation databases
GeneID	1246413.
GenomeReviews	Gene locus TC_0244 in contig AE002160_GR.
KEGG	cmu:TC0244.
TIGR	TC_0244.
Phylogenomic databases
HOGENOM	Q9PL64.
OMA	GSQGHFE.
Enzyme and pathway databases
BioCyc	CMUR243161:TC_0244-MON.
BRENDA	4.2.1.2. 256349.
Family and domain databases
HAMAP	MF_00743. [Tree]
InterPro	IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00979. fumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_CHLMU

Accession

Primary (citable) accession number: Q9PL64

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)