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Q9PL20

- SYI_CHLMU

UniProt

Q9PL20 - SYI_CHLMU

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Protein
Isoleucine--tRNA ligase
Gene
ileS, TC_0288
Organism
Chlamydia muridarum (strain MoPn / Nigg)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei592 – 5921ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCMUR243161:GHYU-302-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:TC_0288
OrganismiChlamydia muridarum (strain MoPn / Nigg)
Taxonomic identifieri243161 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000800: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10361036Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098527Add
BLAST

Proteomic databases

PRIDEiQ9PL20.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi243161.TC0288.

Structurei

3D structure databases

ProteinModelPortaliQ9PL20.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi46 – 5611"HIGH" regionUniRule annotation
Add
BLAST
Motifi589 – 5935"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKESHFDE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PL20-1 [UniParc]FASTAAdd to Basket

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MDNEDKVSFP AKEEKVLTFW KEQNIFQKTL ENRDGSPTFS FYDGPPFATG     50
LPHYGHLLAG TIKDVVCRYA TMDGHYVPRR FGWDCHGVPV EYEVEKSLGL 100
TEPGAIDRFG IANFNEECRK IVFRYVDEWK YFVDRIGRWV DFSATWKTMD 150
LSFMESVWWV FHSLYKQGLV YEGTKVVPFS TKLGTPLSNF EAGQNYKEVD 200
DPSVVAKFAL QDDQGILLAW TTTPWTLVSN MALAVHPGLT YVRIQDKESG 250
EEYILGQESL ARWFPDRESY KWIGQLSGES LVGRRYCPLF PYFQDQQDRG 300
AFRVIPADFI EESEGTGVVH MAPAFGEADF FACQEHNVPL VCPVDNQGCF 350
TSEVTDFVGE YIKFADKGIA RRLKNENKLF YQGTIRHRYP FCWRTDSPLI 400
YKAVNSWFVS VEKVKHKMLK ANESIHWTPG HIKHGRFGKW LEGARDWAIS 450
RNRYWGTPIP IWRSEDGELL VIRSIQELEE LSGQKIVDLH RHFIDEIVIH 500
KNGKSFHRIP YVFDCWFDSG AMPYAQNHYP FERAEETEAR FPADFIAEGL 550
DQTRGWFYTL TVIAAALFDQ PAFKNVIVNG IVLAEDGNKM SKRLNNYPSP 600
KKIMDTYGAD ALRLYLLNSV VVKAEDLRFS DKGVEAVLKQ VLLPLSNALA 650
FYKTYAELYG FSPNETTDLE LAEIDRWILS SLYSLVGKTR ENMAQYDLHA 700
AVSPFIDFIE DLTNWYIRRS RRRFWESEDS PDRRAAFATL YEVLMVFSKI 750
IAPFIPFTAE DMYQQLRVET DPESVHLCDF PHVVLEKILP DLEKKMQDIR 800
EIVALGHSLR KEHKLKVRQP LQHMYIVGAK ERMAALAQVD SLIGEELNVK 850
EVHFCSETPE YVTTLVKPNF RSLGKRVGNR LPEIQKALAG LSQEQIQAFM 900
HNGFMVLSLG EETISLNEED ITVSWEAAPG FVARSSASFV AILDCQLTSP 950
LIMEGIAREI VNKINTMRRN GKLHVSDRIA IRLHAPKIVQ EAFSQYEEYI 1000
CEETLTTSVS FIDDKEGEEW DVNGHAVSLS LEVIGH 1036
Length:1,036
Mass (Da):118,950
Last modified:October 1, 2000 - v1
Checksum:i69E6B05FD54D5E8D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE002160 Genomic DNA. Translation: AAF39156.1.
PIRiF81719.
RefSeqiNP_296667.1. NC_002620.2.

Genome annotation databases

EnsemblBacteriaiAAF39156; AAF39156; TC_0288.
GeneIDi1246458.
KEGGicmu:TC_0288.
PATRICi20371978. VBIChlMur19118_0315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE002160 Genomic DNA. Translation: AAF39156.1 .
PIRi F81719.
RefSeqi NP_296667.1. NC_002620.2.

3D structure databases

ProteinModelPortali Q9PL20.
ModBasei Search...

Protein-protein interaction databases

STRINGi 243161.TC0288.

Proteomic databases

PRIDEi Q9PL20.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAF39156 ; AAF39156 ; TC_0288 .
GeneIDi 1246458.
KEGGi cmu:TC_0288.
PATRICi 20371978. VBIChlMur19118_0315.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KESHFDE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CMUR243161:GHYU-302-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MoPn / Nigg.

Entry informationi

Entry nameiSYI_CHLMU
AccessioniPrimary (citable) accession number: Q9PL20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi