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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Chlamydia muridarum (strain MoPn / Nigg)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei592ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:TC_0288
OrganismiChlamydia muridarum (strain MoPn / Nigg)
Taxonomic identifieri243161 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000000800 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985271 – 1036Isoleucine--tRNA ligaseAdd BLAST1036

Proteomic databases

PRIDEiQ9PL20

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243161.CmurN_010100001463

Structurei

3D structure databases

ProteinModelPortaliQ9PL20
SMRiQ9PL20
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi46 – 56"HIGH" regionAdd BLAST11
Motifi589 – 593"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
KOiK01870
OMAiHLGTAWN

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 2 hits
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q9PL20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNEDKVSFP AKEEKVLTFW KEQNIFQKTL ENRDGSPTFS FYDGPPFATG
60 70 80 90 100
LPHYGHLLAG TIKDVVCRYA TMDGHYVPRR FGWDCHGVPV EYEVEKSLGL
110 120 130 140 150
TEPGAIDRFG IANFNEECRK IVFRYVDEWK YFVDRIGRWV DFSATWKTMD
160 170 180 190 200
LSFMESVWWV FHSLYKQGLV YEGTKVVPFS TKLGTPLSNF EAGQNYKEVD
210 220 230 240 250
DPSVVAKFAL QDDQGILLAW TTTPWTLVSN MALAVHPGLT YVRIQDKESG
260 270 280 290 300
EEYILGQESL ARWFPDRESY KWIGQLSGES LVGRRYCPLF PYFQDQQDRG
310 320 330 340 350
AFRVIPADFI EESEGTGVVH MAPAFGEADF FACQEHNVPL VCPVDNQGCF
360 370 380 390 400
TSEVTDFVGE YIKFADKGIA RRLKNENKLF YQGTIRHRYP FCWRTDSPLI
410 420 430 440 450
YKAVNSWFVS VEKVKHKMLK ANESIHWTPG HIKHGRFGKW LEGARDWAIS
460 470 480 490 500
RNRYWGTPIP IWRSEDGELL VIRSIQELEE LSGQKIVDLH RHFIDEIVIH
510 520 530 540 550
KNGKSFHRIP YVFDCWFDSG AMPYAQNHYP FERAEETEAR FPADFIAEGL
560 570 580 590 600
DQTRGWFYTL TVIAAALFDQ PAFKNVIVNG IVLAEDGNKM SKRLNNYPSP
610 620 630 640 650
KKIMDTYGAD ALRLYLLNSV VVKAEDLRFS DKGVEAVLKQ VLLPLSNALA
660 670 680 690 700
FYKTYAELYG FSPNETTDLE LAEIDRWILS SLYSLVGKTR ENMAQYDLHA
710 720 730 740 750
AVSPFIDFIE DLTNWYIRRS RRRFWESEDS PDRRAAFATL YEVLMVFSKI
760 770 780 790 800
IAPFIPFTAE DMYQQLRVET DPESVHLCDF PHVVLEKILP DLEKKMQDIR
810 820 830 840 850
EIVALGHSLR KEHKLKVRQP LQHMYIVGAK ERMAALAQVD SLIGEELNVK
860 870 880 890 900
EVHFCSETPE YVTTLVKPNF RSLGKRVGNR LPEIQKALAG LSQEQIQAFM
910 920 930 940 950
HNGFMVLSLG EETISLNEED ITVSWEAAPG FVARSSASFV AILDCQLTSP
960 970 980 990 1000
LIMEGIAREI VNKINTMRRN GKLHVSDRIA IRLHAPKIVQ EAFSQYEEYI
1010 1020 1030
CEETLTTSVS FIDDKEGEEW DVNGHAVSLS LEVIGH
Length:1,036
Mass (Da):118,950
Last modified:October 1, 2000 - v1
Checksum:i69E6B05FD54D5E8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE002160 Genomic DNA Translation: AAF39156.1
PIRiF81719
RefSeqiWP_010230051.1, NZ_ACOV01000003.1

Genome annotation databases

EnsemblBacteriaiAAF39156; AAF39156; TC_0288
GeneIDi1246458
KEGGicmu:TC_0288

Similar proteinsi

Entry informationi

Entry nameiSYI_CHLMU
AccessioniPrimary (citable) accession number: Q9PL20
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 1, 2000
Last modified: March 28, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health