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Q9PL20

- SYI_CHLMU

UniProt

Q9PL20 - SYI_CHLMU

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Chlamydia muridarum (strain MoPn / Nigg)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei592 – 5921ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCMUR243161:GHYU-302-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:TC_0288
    OrganismiChlamydia muridarum (strain MoPn / Nigg)
    Taxonomic identifieri243161 [NCBI]
    Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
    ProteomesiUP000000800: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10361036Isoleucine--tRNA ligasePRO_0000098527Add
    BLAST

    Proteomic databases

    PRIDEiQ9PL20.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi243161.TC0288.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9PL20.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi46 – 5611"HIGH" regionAdd
    BLAST
    Motifi589 – 5935"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiKESHFDE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9PL20-1 [UniParc]FASTAAdd to Basket

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    MDNEDKVSFP AKEEKVLTFW KEQNIFQKTL ENRDGSPTFS FYDGPPFATG     50
    LPHYGHLLAG TIKDVVCRYA TMDGHYVPRR FGWDCHGVPV EYEVEKSLGL 100
    TEPGAIDRFG IANFNEECRK IVFRYVDEWK YFVDRIGRWV DFSATWKTMD 150
    LSFMESVWWV FHSLYKQGLV YEGTKVVPFS TKLGTPLSNF EAGQNYKEVD 200
    DPSVVAKFAL QDDQGILLAW TTTPWTLVSN MALAVHPGLT YVRIQDKESG 250
    EEYILGQESL ARWFPDRESY KWIGQLSGES LVGRRYCPLF PYFQDQQDRG 300
    AFRVIPADFI EESEGTGVVH MAPAFGEADF FACQEHNVPL VCPVDNQGCF 350
    TSEVTDFVGE YIKFADKGIA RRLKNENKLF YQGTIRHRYP FCWRTDSPLI 400
    YKAVNSWFVS VEKVKHKMLK ANESIHWTPG HIKHGRFGKW LEGARDWAIS 450
    RNRYWGTPIP IWRSEDGELL VIRSIQELEE LSGQKIVDLH RHFIDEIVIH 500
    KNGKSFHRIP YVFDCWFDSG AMPYAQNHYP FERAEETEAR FPADFIAEGL 550
    DQTRGWFYTL TVIAAALFDQ PAFKNVIVNG IVLAEDGNKM SKRLNNYPSP 600
    KKIMDTYGAD ALRLYLLNSV VVKAEDLRFS DKGVEAVLKQ VLLPLSNALA 650
    FYKTYAELYG FSPNETTDLE LAEIDRWILS SLYSLVGKTR ENMAQYDLHA 700
    AVSPFIDFIE DLTNWYIRRS RRRFWESEDS PDRRAAFATL YEVLMVFSKI 750
    IAPFIPFTAE DMYQQLRVET DPESVHLCDF PHVVLEKILP DLEKKMQDIR 800
    EIVALGHSLR KEHKLKVRQP LQHMYIVGAK ERMAALAQVD SLIGEELNVK 850
    EVHFCSETPE YVTTLVKPNF RSLGKRVGNR LPEIQKALAG LSQEQIQAFM 900
    HNGFMVLSLG EETISLNEED ITVSWEAAPG FVARSSASFV AILDCQLTSP 950
    LIMEGIAREI VNKINTMRRN GKLHVSDRIA IRLHAPKIVQ EAFSQYEEYI 1000
    CEETLTTSVS FIDDKEGEEW DVNGHAVSLS LEVIGH 1036
    Length:1,036
    Mass (Da):118,950
    Last modified:October 1, 2000 - v1
    Checksum:i69E6B05FD54D5E8D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE002160 Genomic DNA. Translation: AAF39156.1.
    PIRiF81719.
    RefSeqiNP_296667.1. NC_002620.2.

    Genome annotation databases

    EnsemblBacteriaiAAF39156; AAF39156; TC_0288.
    GeneIDi1246458.
    KEGGicmu:TC_0288.
    PATRICi20371978. VBIChlMur19118_0315.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE002160 Genomic DNA. Translation: AAF39156.1 .
    PIRi F81719.
    RefSeqi NP_296667.1. NC_002620.2.

    3D structure databases

    ProteinModelPortali Q9PL20.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243161.TC0288.

    Proteomic databases

    PRIDEi Q9PL20.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAF39156 ; AAF39156 ; TC_0288 .
    GeneIDi 1246458.
    KEGGi cmu:TC_0288.
    PATRICi 20371978. VBIChlMur19118_0315.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi KESHFDE.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CMUR243161:GHYU-302-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MoPn / Nigg.

    Entry informationi

    Entry nameiSYI_CHLMU
    AccessioniPrimary (citable) accession number: Q9PL20
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3