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Q9PL20

- SYI_CHLMU

UniProt

Q9PL20 - SYI_CHLMU

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Chlamydia muridarum (strain MoPn / Nigg)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei592 – 5921ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCMUR243161:GHYU-302-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:TC_0288
OrganismiChlamydia muridarum (strain MoPn / Nigg)
Taxonomic identifieri243161 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
ProteomesiUP000000800: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10361036Isoleucine--tRNA ligasePRO_0000098527Add
BLAST

Proteomic databases

PRIDEiQ9PL20.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243161.TC0288.

Structurei

3D structure databases

ProteinModelPortaliQ9PL20.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi46 – 5611"HIGH" regionAdd
BLAST
Motifi589 – 5935"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKESHFDE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PL20-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDNEDKVSFP AKEEKVLTFW KEQNIFQKTL ENRDGSPTFS FYDGPPFATG
60 70 80 90 100
LPHYGHLLAG TIKDVVCRYA TMDGHYVPRR FGWDCHGVPV EYEVEKSLGL
110 120 130 140 150
TEPGAIDRFG IANFNEECRK IVFRYVDEWK YFVDRIGRWV DFSATWKTMD
160 170 180 190 200
LSFMESVWWV FHSLYKQGLV YEGTKVVPFS TKLGTPLSNF EAGQNYKEVD
210 220 230 240 250
DPSVVAKFAL QDDQGILLAW TTTPWTLVSN MALAVHPGLT YVRIQDKESG
260 270 280 290 300
EEYILGQESL ARWFPDRESY KWIGQLSGES LVGRRYCPLF PYFQDQQDRG
310 320 330 340 350
AFRVIPADFI EESEGTGVVH MAPAFGEADF FACQEHNVPL VCPVDNQGCF
360 370 380 390 400
TSEVTDFVGE YIKFADKGIA RRLKNENKLF YQGTIRHRYP FCWRTDSPLI
410 420 430 440 450
YKAVNSWFVS VEKVKHKMLK ANESIHWTPG HIKHGRFGKW LEGARDWAIS
460 470 480 490 500
RNRYWGTPIP IWRSEDGELL VIRSIQELEE LSGQKIVDLH RHFIDEIVIH
510 520 530 540 550
KNGKSFHRIP YVFDCWFDSG AMPYAQNHYP FERAEETEAR FPADFIAEGL
560 570 580 590 600
DQTRGWFYTL TVIAAALFDQ PAFKNVIVNG IVLAEDGNKM SKRLNNYPSP
610 620 630 640 650
KKIMDTYGAD ALRLYLLNSV VVKAEDLRFS DKGVEAVLKQ VLLPLSNALA
660 670 680 690 700
FYKTYAELYG FSPNETTDLE LAEIDRWILS SLYSLVGKTR ENMAQYDLHA
710 720 730 740 750
AVSPFIDFIE DLTNWYIRRS RRRFWESEDS PDRRAAFATL YEVLMVFSKI
760 770 780 790 800
IAPFIPFTAE DMYQQLRVET DPESVHLCDF PHVVLEKILP DLEKKMQDIR
810 820 830 840 850
EIVALGHSLR KEHKLKVRQP LQHMYIVGAK ERMAALAQVD SLIGEELNVK
860 870 880 890 900
EVHFCSETPE YVTTLVKPNF RSLGKRVGNR LPEIQKALAG LSQEQIQAFM
910 920 930 940 950
HNGFMVLSLG EETISLNEED ITVSWEAAPG FVARSSASFV AILDCQLTSP
960 970 980 990 1000
LIMEGIAREI VNKINTMRRN GKLHVSDRIA IRLHAPKIVQ EAFSQYEEYI
1010 1020 1030
CEETLTTSVS FIDDKEGEEW DVNGHAVSLS LEVIGH
Length:1,036
Mass (Da):118,950
Last modified:October 1, 2000 - v1
Checksum:i69E6B05FD54D5E8D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE002160 Genomic DNA. Translation: AAF39156.1.
PIRiF81719.
RefSeqiNP_296667.1. NC_002620.2.

Genome annotation databases

EnsemblBacteriaiAAF39156; AAF39156; TC_0288.
GeneIDi1246458.
KEGGicmu:TC_0288.
PATRICi20371978. VBIChlMur19118_0315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE002160 Genomic DNA. Translation: AAF39156.1 .
PIRi F81719.
RefSeqi NP_296667.1. NC_002620.2.

3D structure databases

ProteinModelPortali Q9PL20.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243161.TC0288.

Proteomic databases

PRIDEi Q9PL20.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAF39156 ; AAF39156 ; TC_0288 .
GeneIDi 1246458.
KEGGi cmu:TC_0288.
PATRICi 20371978. VBIChlMur19118_0315.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KESHFDE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CMUR243161:GHYU-302-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MoPn / Nigg.

Entry informationi

Entry nameiSYI_CHLMU
AccessioniPrimary (citable) accession number: Q9PL20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3