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Protein

DNA gyrase subunit A

Gene

gyrA

Organism
Chlamydia muridarum (strain MoPn / Nigg)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.UniRule annotation

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei122O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA gyrase subunit AUniRule annotation (EC:5.99.1.3UniRule annotation)
Gene namesi
Name:gyrAUniRule annotation
Ordered Locus Names:TC_0461
OrganismiChlamydia muridarum (strain MoPn / Nigg)
Taxonomic identifieri243161 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000000800 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001452281 – 833DNA gyrase subunit AAdd BLAST833

Proteomic databases

PRIDEiQ9PKK4.

Interactioni

Subunit structurei

Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.UniRule annotation

Protein-protein interaction databases

STRINGi243161.CmurN_010100002328.

Structurei

3D structure databases

ProteinModelPortaliQ9PKK4.
SMRiQ9PKK4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi527 – 533GyrA-boxUniRule annotation7

Sequence similaritiesi

Belongs to the type II topoisomerase GyrA/ParC subunit family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C24. Bacteria.
COG0188. LUCA.
KOiK02469.
OMAiTHHWLLF.

Family and domain databases

Gene3Di1.10.268.10. 1 hit.
2.120.10.90. 1 hit.
3.30.1360.40. 1 hit.
3.90.199.10. 1 hit.
HAMAPiMF_01897. GyrA. 1 hit.
InterProiView protein in InterPro
IPR024946. Arg_repress-like_C.
IPR005743. GyrA.
IPR006691. GyrA/parC_rep.
IPR035516. Gyrase/topoIV_suA_C.
IPR013760. Topo_IIA-like_dom_sf.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a_sf.
PfamiView protein in Pfam
PF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
SMARTiView protein in SMART
SM00434. TOP4c. 1 hit.
SUPFAMiSSF101904. SSF101904. 1 hit.
SSF56719. SSF56719. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9PKK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNKEEIIVP KNLEEEMKES YLRYSMSVII SRALPDVRDG LKPSQRRILY
60 70 80 90 100
AMKQLNLTPG AKHRKCAKIC GDTSGDYHPH GEAVIYPTLV RMAQDWAMRY
110 120 130 140 150
PLVDGQGNFG SIDGDPAAAM RYTEARLTHS SIFLLEDLDK DTVDMVPNYD
160 170 180 190 200
ETKYEPVVFP SKFPNLLCNG SSGIAVGMAT NIPPHNLGEL IEATLLVLAN
210 220 230 240 250
PQTSIEEILE VMPGPDFPTG GIICGTEGIR STYYTGRGKL RLRARIHVEE
260 270 280 290 300
NSDKQRENII LTEMPYNVNK SRLVEQIAEL INEKTLTGIS DVRDESDKDG
310 320 330 340 350
IRVVLELKKG ESSEVIINRL YKFTDVQVTF GANMLALDKN LPRTMSIHRM
360 370 380 390 400
ISAWIRHRMD VIRRRTRYEL NKAETRAHIL EGFLKALSCM DEVVKTIRES
410 420 430 440 450
SNKEHAKQQL VELFGFSEAQ SLAILELRLY QLTGLEIDKV QKEYNELLEK
460 470 480 490 500
IAYYRRVLAE EELVKDIIRE ELQELHKVHK TPRRTTIEMD AGDVRDIEDI
510 520 530 540 550
IADESVIITI SGDDYVKRMP VKVFREQKRG GQGVTGFDMK KGSDFLKAVY
560 570 580 590 600
SASTKDYLLI FTNMGQCYWL KVWQLPEGER RAKGKPIINF LEGIRPGEQV
610 620 630 640 650
AAVLNVKRFE QGEYLLLATK KGVVKKVSLD AFGSPRKKGI RALEIDDGDE
660 670 680 690 700
LIAARHIVND EEKVMLFTRL GMAVRFPHDK VRPMGRAARG VRGVSLKNEE
710 720 730 740 750
DFVVSCQVVT DDQSVLVVCD NGFGKRSLVC DFRETNRGSV GVRSILINQR
760 770 780 790 800
NGDVLGAISV TDFDSILLMS AQGQAIRINM QDVRVMGRAT QGVRLVNLRE
810 820 830
GDTLVAMEKL SVNTESGETE ESVSIQVGHA VEE
Length:833
Mass (Da):93,884
Last modified:October 1, 2000 - v1
Checksum:iD168FE5950D8D221
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE002160 Genomic DNA. Translation: AAF39312.1.
PIRiH81700.
RefSeqiWP_010230505.1. NZ_ACOV01000003.1.

Genome annotation databases

EnsemblBacteriaiAAF39312; AAF39312; TC_0461.
GeneIDi1245816.
KEGGicmu:TC_0461.

Similar proteinsi

Entry informationi

Entry nameiGYRA_CHLMU
AccessioniPrimary (citable) accession number: Q9PKK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 1, 2000
Last modified: November 22, 2017
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families