Reviewed, UniProtKB/Swiss-Prot Q9PKK4 (GYRA_CHLMU)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit A
    EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	TC_0461

Organism

Chlamydia muridarum [Complete proteome] [HAMAP]

Taxonomic identifier

83560 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia

Protein attributes

Sequence length	833 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 833	833	DNA gyrase subunit A		PRO_0000145228
Sites
	Active site	122	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PKK4-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: D168FE5950D8D221	FASTA	833	93,884
	10 20 30 40 50 60 MLNKEEIIVP KNLEEEMKES YLRYSMSVII SRALPDVRDG LKPSQRRILY AMKQLNLTPG 70 80 90 100 110 120 AKHRKCAKIC GDTSGDYHPH GEAVIYPTLV RMAQDWAMRY PLVDGQGNFG SIDGDPAAAM 130 140 150 160 170 180 RYTEARLTHS SIFLLEDLDK DTVDMVPNYD ETKYEPVVFP SKFPNLLCNG SSGIAVGMAT 190 200 210 220 230 240 NIPPHNLGEL IEATLLVLAN PQTSIEEILE VMPGPDFPTG GIICGTEGIR STYYTGRGKL 250 260 270 280 290 300 RLRARIHVEE NSDKQRENII LTEMPYNVNK SRLVEQIAEL INEKTLTGIS DVRDESDKDG 310 320 330 340 350 360 IRVVLELKKG ESSEVIINRL YKFTDVQVTF GANMLALDKN LPRTMSIHRM ISAWIRHRMD 370 380 390 400 410 420 VIRRRTRYEL NKAETRAHIL EGFLKALSCM DEVVKTIRES SNKEHAKQQL VELFGFSEAQ 430 440 450 460 470 480 SLAILELRLY QLTGLEIDKV QKEYNELLEK IAYYRRVLAE EELVKDIIRE ELQELHKVHK 490 500 510 520 530 540 TPRRTTIEMD AGDVRDIEDI IADESVIITI SGDDYVKRMP VKVFREQKRG GQGVTGFDMK 550 560 570 580 590 600 KGSDFLKAVY SASTKDYLLI FTNMGQCYWL KVWQLPEGER RAKGKPIINF LEGIRPGEQV 610 620 630 640 650 660 AAVLNVKRFE QGEYLLLATK KGVVKKVSLD AFGSPRKKGI RALEIDDGDE LIAARHIVND 670 680 690 700 710 720 EEKVMLFTRL GMAVRFPHDK VRPMGRAARG VRGVSLKNEE DFVVSCQVVT DDQSVLVVCD 730 740 750 760 770 780 NGFGKRSLVC DFRETNRGSV GVRSILINQR NGDVLGAISV TDFDSILLMS AQGQAIRINM 790 800 810 820 830 QDVRVMGRAT QGVRLVNLRE GDTLVAMEKL SVNTESGETE ESVSIQVGHA VEE

References

[1]

"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MoPn / Nigg.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE002160 Genomic DNA. Translation: AAF39312.1.
PIR	H81700.
RefSeq	NP_296838.1.
3D structure databases
HSSP	HSSP built from PDB template 1AB4 based on UniProtKB P09097.
ModBase	Search...
Genome annotation databases
GeneID	1245816.
GenomeReviews	Gene locus TC_0461 in contig AE002160_GR.
KEGG	cmu:TC0461.
TIGR	TC_0461.
Phylogenomic databases
HOGENOM	Q9PKK4.
OMA	ELGNDWN.
Enzyme and pathway databases
BioCyc	CMUR243161:TC_0461-MON.
BRENDA	5.99.1.3. 256349.
Family and domain databases
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
ProDom	PD000742. DNA_topoisoIV. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_CHLMU

Accession

Primary (citable) accession number: Q9PKK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)