Q9PKF1 (LPXD_CHLMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	TC_0514

Organism

Chlamydia muridarum [Complete proteome] [HAMAP]

Taxonomic identifier

83560 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_0000059662
Sites
	Active site	247	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PKF1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: A0457784145E63D8	FASTA	354	38,325
	10 20 30 40 50 60 MSQPVYSLKQ LADFLNVEFQ GNGATLLSGV EEIGEAKAAH VTFLDNEKYA KHLKSSEAGA 70 80 90 100 110 120 IILSRTQFQK YRELNKNFLI VSESPSLVFQ KCLELFIAPV DSGFPGIHPT AVIHPTAIIE 130 140 150 160 170 180 EHVCIEPYVV ICQHARIGAA CHIGTGSVIG AHSSIGEHSY IYPRVVVRER VSIGKRVIIQ 190 200 210 220 230 240 PGAIIGSCGF GYVTSAFGQH KHLKHLGTVI IEDDVEIGAN TTIDRGRFKH SIVREGSKID 250 260 270 280 290 300 NLVQIAHQVE VGQHSMVVAQ AGIAGSTKIG NHVIIGGQAG VTGHICIADH VIMMAQTGVT 310 320 330 340 350 KSITSPGIYG GAPARPYQEV HRQVAKIRNL PRLEERIASL EKLVQKLEAL SEQH

References

[1]

"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MoPn / Nigg.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE002160 Genomic DNA. Translation: AAF39356.1.
PIR	B81694.
RefSeq	NP_296891.1. NC_002620.2.
3D structure databases
ProteinModelPortal	Q9PKF1.
SMR	Q9PKF1. Positions 1-346.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1245874.
GenomeReviews	Gene locus TC_0514 in contig AE002160_GR.
KEGG	cmu:TC0514.
PATRIC	20372472. VBIChlMur19118_0552.
TIGR	TC_0514.
Phylogenomic databases
HOGENOM	HBG469615.
OMA	SYPAKIM.
PhylomeDB	Q9PKF1.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	CMUR243161:TC_0514-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 4 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_CHLMU

Accession

Primary (citable) accession number: Q9PKF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program