Reviewed, UniProtKB/Swiss-Prot Q9PJZ1 (RISA_CHLMU)

Names and origin

Protein names

Recommended name:
    Riboflavin synthase alpha chain
    EC=2.5.1.9

Gene names

Name:	ribE
Synonyms:	ribC
Ordered Locus Names:	TC_0685

Organism

Chlamydia muridarum [Complete proteome] [HAMAP]

Taxonomic identifier

83560 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyzes the dismutation of 6,7-dimethyl-8-lumazine to riboflavin and 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione By similarity.
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.
Subunit structure	Oligomer that consist of 3 alpha subunits and 60 beta subunits By similarity.
Sequence similarities	Contains 2 lumazine-binding repeats.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Domain	Repeat
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	Riboflavin synthase alpha chain		PRO_0000068163
Regions
	Repeat	1 – 95	95	Lumazine-binding 1
	Repeat	96 – 188	93	Lumazine-binding 2

Sequences

	Sequence		Length	Mass (Da)
	Q9PJZ1-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 8C6218EFBD94C318	FASTA	198	21,761
	10 20 30 40 50 60 MFSGIIQEVA RVDLIHHYGD SMEIGIFARN LVDGVPGSSI AVDGICLTLV KREFELLFFD 70 80 90 100 110 120 VTEETMACTT IKNYTVGSMV NLERSVRLGD EIGGHFVSGH VCGVGTIIAV EKSYMFFKAP 130 140 150 160 170 180 TNLVPYVLEK GFIAIDGISL TIAQVRGDIF SVSVIPETRA RTSLGYKQVG SHVNMEPDMM 190 TKMQVDTVMR FQAEKIGK

References

[1]

"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MoPn / Nigg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE002160 Genomic DNA. Translation: AAF39502.1.
PIR	G81675.
RefSeq	NP_297059.1.
3D structure databases
SMR	Q9PJZ1. Positions 1-185.
ModBase	Search...
Genome annotation databases
GeneID	1246046.
GenomeReviews	Gene locus TC_0685 in contig AE002160_GR.
KEGG	cmu:TC0685.
TIGR	TC_0685.
Phylogenomic databases
HOGENOM	HBG289809.
OMA	DEIGGHS.
PhylomeDB	Q9PJZ1.
Enzyme and pathway databases
BioCyc	CMUR243161:TC_0685-MONOMER.
BRENDA	2.5.1.9. 256349.
Family and domain databases
InterPro	IPR001783. Lumazine_bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
PANTHER	PTHR21098. Lumazine_bd. 1 hit.
Pfam	PF00677. Lum_binding. 2 hits. [Graphical view]
PIRSF	PIRSF000498. Riboflavin_syn_A. 1 hit.
TIGRFAMs	TIGR00187. ribE. 1 hit.
PROSITE	PS51177. LUMAZINE_BIND. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RISA_CHLMU

Accession

Primary (citable) accession number: Q9PJZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)