Reviewed, UniProtKB/Swiss-Prot Q9PJ53 (AROQ_CAMJE)

Names and origin

Protein names

Recommended name:
    3-dehydroquinate dehydratase
      Short name=3-dehydroquinase
    EC=4.2.1.10
Alternative name(s):
    Type II DHQase

Gene names

Name:	aroQ
Ordered Locus Names:	Cj0066c

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	159 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes a trans-dehydration via an enolate intermediate By similarity.
Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00169
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 3/7. HAMAP MF_00169
Subunit structure	Homododecamer By similarity.
Sequence similarities	Belongs to the type-II 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: HAMAP protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
fliY	Q0PC74	1	EBI-1193003,EBI-1191111

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 159	159	3-dehydroquinate dehydratase HAMAP MF_00169		PRO_0000159886
Regions
	Region	100 – 101	2	Substrate binding By similarity
Sites
	Active site	22	1	Proton acceptor By similarity
	Active site	99	1	Proton donor By similarity
	Binding site	73	1	Substrate By similarity
	Binding site	79	1	Substrate By similarity
	Binding site	86	1	Substrate By similarity
	Binding site	110	1	Substrate By similarity
	Site	17	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PJ53-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 9402903782B127EA	FASTA	159	17,595
	10 20 30 40 50 60 MKIMIIQGPN VNMLGVREVG IYGAMKMEEI HEQMKLAASQ NNVELDFFQS NFEGEIVDKI 70 80 90 100 110 120 QECLGTVDGI IINAAGYTHT SVAIRDAIAA VALPTIEVHI SNVYRREEFR QKSLIAPVCS 130 140 150 GTIVGFGPFG YHLALMGIIQ ICEQIKNLRA MQQAQQTNK

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL111168 Genomic DNA. Translation: CAL34240.1.
PIR	F81422.
RefSeq	YP_002343530.1.
3D structure databases
HSSP	HSSP built from PDB template 1J2Y based on UniProtKB Q48255.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PJ53. 13 interactions.
Genome annotation databases
GeneID	904397.
GenomeReviews	Gene locus Cj0066c in contig AL111168_GR.
KEGG	cje:Cj0066c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q9PJ53. LIDWIHQ.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0066C-MON.
BRENDA	4.2.1.10. 255835.
Family and domain databases
HAMAP	MF_00169. [Tree]
InterPro	IPR001874. DHquinase_II. IPR018509. DHquinase_II_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.9100. DHquinase_II. 1 hit.
PANTHER	PTHR21272. DHquinase_II. 1 hit.
Pfam	PF01220. DHquinase_II. 1 hit. [Graphical view]
PIRSF	PIRSF001399. DHquinase_II. 1 hit.
ProDom	PD004527. DHquinase_II. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01088. aroQ. 1 hit.
PROSITE	PS01029. DEHYDROQUINASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROQ_CAMJE

Accession

Primary (citable) accession number: Q9PJ53
Secondary accession number(s): Q0PC67

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)