Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamate 5-kinase

Gene

proB

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei7ATPUniRule annotation1
Binding sitei45SubstrateUniRule annotation1
Binding sitei130SubstrateUniRule annotation1
Binding sitei142Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi162 – 163ATP2
Nucleotide bindingi168 – 173ATP6
Nucleotide bindingi204 – 210ATP7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCJEJ192222:G1G1F-91-MONOMER
UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:Cj0097
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001096561 – 251Glutamate 5-kinaseAdd BLAST251

Proteomic databases

PaxDbiQ9PJ29
PRIDEiQ9PJ29

Interactioni

Protein-protein interaction databases

IntActiQ9PJ29, 9 interactors
STRINGi192222.Cj0097

Structurei

Secondary structure

1251
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi10 – 13Combined sources4
Beta strandi16 – 19Combined sources4
Helixi21 – 37Combined sources17
Beta strandi38 – 44Combined sources7
Helixi47 – 54Combined sources8
Beta strandi59 – 61Combined sources3
Helixi62 – 84Combined sources23
Helixi85 – 87Combined sources3
Beta strandi91 – 96Combined sources6
Helixi99 – 102Combined sources4
Helixi104 – 119Combined sources16
Beta strandi123 – 128Combined sources6
Turni130 – 132Combined sources3
Helixi135 – 138Combined sources4
Turni140 – 142Combined sources3
Helixi143 – 152Combined sources10
Beta strandi156 – 164Combined sources9
Beta strandi168 – 170Combined sources3
Turni172 – 174Combined sources3
Beta strandi183 – 185Combined sources3
Helixi189 – 191Combined sources3
Helixi206 – 219Combined sources14
Beta strandi223 – 231Combined sources9
Helixi233 – 240Combined sources8
Beta strandi246 – 250Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AKOX-ray2.20A/B/C/D1-251[»]
ProteinModelPortaliQ9PJ29
SMRiQ9PJ29
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9PJ29

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
HOGENOMiHOG000246368
KOiK00931
OMAiADDFDSR

Family and domain databases

Gene3Di3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SUPFAMiSSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9PJ29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRIVVKVGS HVISEENTLS FERLKNLVAF LAKLMEKYEV ILVTSAAISA
60 70 80 90 100
GHTKLDIDRK NLINKQVLAA IGQPFLISVY NELLAKFNKL GGQILLTGKD
110 120 130 140 150
FDSRKATKHA KNAIDMMINL GILPIINEND ATAIEEIVFG DNDSLSAYAT
160 170 180 190 200
HFFDADLLVI LSDIDGFYDK NPSEFSDAKR LEKITHIKEE WLQATIKTGS
210 220 230 240 250
EHGTGGIVTK LKAAKFLLEH NKKMFLASGF DLSVAKTFLL EDKQIGGTLF

E
Length:251
Mass (Da):27,843
Last modified:October 1, 2000 - v1
Checksum:iD07802E92023A241
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA Translation: CAL34268.1
PIRiA81426
RefSeqiWP_002851769.1, NC_002163.1
YP_002343557.1, NC_002163.1

Genome annotation databases

EnsemblBacteriaiCAL34268; CAL34268; Cj0097
GeneIDi904426
KEGGicje:Cj0097
PATRICifig|192222.6.peg.95

Similar proteinsi

Entry informationi

Entry nameiPROB_CAMJE
AccessioniPrimary (citable) accession number: Q9PJ29
Secondary accession number(s): Q0PC40
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health