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Q9PJ29

- PROB_CAMJE

UniProt

Q9PJ29 - PROB_CAMJE

Protein

Glutamate 5-kinase

Gene

proB

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

    Catalytic activityi

    ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei7 – 71ATPUniRule annotation
    Binding sitei45 – 451SubstrateUniRule annotation
    Binding sitei130 – 1301SubstrateUniRule annotation
    Binding sitei142 – 1421Substrate; via amide nitrogenUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi162 – 1632ATP
    Nucleotide bindingi168 – 1736ATP
    Nucleotide bindingi204 – 2107ATP

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate 5-kinase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. L-proline biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Proline biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCJEJ192222:GJTS-93-MONOMER.
    UniPathwayiUPA00098; UER00359.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
    Alternative name(s):
    Gamma-glutamyl kinaseUniRule annotation
    Short name:
    GKUniRule annotation
    Gene namesi
    Name:proBUniRule annotation
    Ordered Locus Names:Cj0097
    OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
    Taxonomic identifieri192222 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
    ProteomesiUP000000799: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 251251Glutamate 5-kinasePRO_0000109656Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    IntActiQ9PJ29. 9 interactions.
    STRINGi192222.Cj0097.

    Structurei

    Secondary structure

    1
    251
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 86
    Helixi10 – 134
    Beta strandi16 – 194
    Helixi21 – 3717
    Beta strandi38 – 447
    Helixi47 – 548
    Beta strandi59 – 613
    Helixi62 – 8423
    Helixi85 – 873
    Beta strandi91 – 966
    Helixi99 – 1024
    Helixi104 – 11916
    Beta strandi123 – 1286
    Turni130 – 1323
    Helixi135 – 1384
    Turni140 – 1423
    Helixi143 – 15210
    Beta strandi156 – 1649
    Beta strandi168 – 1703
    Turni172 – 1743
    Beta strandi183 – 1853
    Helixi189 – 1913
    Helixi206 – 21914
    Beta strandi223 – 2319
    Helixi233 – 2408
    Beta strandi246 – 2505

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2AKOX-ray2.20A/B/C/D1-251[»]
    ProteinModelPortaliQ9PJ29.
    SMRiQ9PJ29. Positions 2-251.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9PJ29.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamate 5-kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0263.
    HOGENOMiHOG000246368.
    KOiK00931.
    OMAiADDFDSR.
    OrthoDBiEOG6PGK7G.

    Family and domain databases

    Gene3Di3.40.1160.10. 1 hit.
    HAMAPiMF_00456. ProB.
    InterProiIPR001048. Asp/Glu/Uridylate_kinase.
    IPR001057. Glu/AcGlu_kinase.
    IPR011529. Glu_5kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    [Graphical view]
    PfamiPF00696. AA_kinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000729. GK. 1 hit.
    PRINTSiPR00474. GLU5KINASE.
    SUPFAMiSSF53633. SSF53633. 1 hit.
    TIGRFAMsiTIGR01027. proB. 1 hit.
    PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9PJ29-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKRIVVKVGS HVISEENTLS FERLKNLVAF LAKLMEKYEV ILVTSAAISA    50
    GHTKLDIDRK NLINKQVLAA IGQPFLISVY NELLAKFNKL GGQILLTGKD 100
    FDSRKATKHA KNAIDMMINL GILPIINEND ATAIEEIVFG DNDSLSAYAT 150
    HFFDADLLVI LSDIDGFYDK NPSEFSDAKR LEKITHIKEE WLQATIKTGS 200
    EHGTGGIVTK LKAAKFLLEH NKKMFLASGF DLSVAKTFLL EDKQIGGTLF 250
    E 251
    Length:251
    Mass (Da):27,843
    Last modified:October 1, 2000 - v1
    Checksum:iD07802E92023A241
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL111168 Genomic DNA. Translation: CAL34268.1.
    PIRiA81426.
    RefSeqiYP_002343557.1. NC_002163.1.

    Genome annotation databases

    EnsemblBacteriaiCAL34268; CAL34268; Cj0097.
    GeneIDi904426.
    KEGGicje:Cj0097.
    PATRICi20057121. VBICamJej33762_0095.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL111168 Genomic DNA. Translation: CAL34268.1 .
    PIRi A81426.
    RefSeqi YP_002343557.1. NC_002163.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2AKO X-ray 2.20 A/B/C/D 1-251 [» ]
    ProteinModelPortali Q9PJ29.
    SMRi Q9PJ29. Positions 2-251.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9PJ29. 9 interactions.
    STRINGi 192222.Cj0097.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAL34268 ; CAL34268 ; Cj0097 .
    GeneIDi 904426.
    KEGGi cje:Cj0097.
    PATRICi 20057121. VBICamJej33762_0095.

    Phylogenomic databases

    eggNOGi COG0263.
    HOGENOMi HOG000246368.
    KOi K00931.
    OMAi ADDFDSR.
    OrthoDBi EOG6PGK7G.

    Enzyme and pathway databases

    UniPathwayi UPA00098 ; UER00359 .
    BioCyci CJEJ192222:GJTS-93-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q9PJ29.

    Family and domain databases

    Gene3Di 3.40.1160.10. 1 hit.
    HAMAPi MF_00456. ProB.
    InterProi IPR001048. Asp/Glu/Uridylate_kinase.
    IPR001057. Glu/AcGlu_kinase.
    IPR011529. Glu_5kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    [Graphical view ]
    Pfami PF00696. AA_kinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000729. GK. 1 hit.
    PRINTSi PR00474. GLU5KINASE.
    SUPFAMi SSF53633. SSF53633. 1 hit.
    TIGRFAMsi TIGR01027. proB. 1 hit.
    PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NCTC 11168.
    2. "Crystal structure of glutamate 5-kinase from Campylobacter jejuni."
      Gorman J., Shapiro L.
      Submitted (AUG-2005) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH ADP.

    Entry informationi

    Entry nameiPROB_CAMJE
    AccessioniPrimary (citable) accession number: Q9PJ29
    Secondary accession number(s): Q0PC40
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3