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Protein

Glutamate 5-kinase

Gene

proB

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei7ATPUniRule annotation1
Binding sitei45SubstrateUniRule annotation1
Binding sitei130SubstrateUniRule annotation1
Binding sitei142Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi162 – 163ATP2
Nucleotide bindingi168 – 173ATP6
Nucleotide bindingi204 – 210ATP7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-90-MONOMER.
UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:Cj0097
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001096561 – 251Glutamate 5-kinaseAdd BLAST251

Proteomic databases

PaxDbiQ9PJ29.

Interactioni

Protein-protein interaction databases

IntActiQ9PJ29. 9 interactors.
STRINGi192222.Cj0097.

Structurei

Secondary structure

1251
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi10 – 13Combined sources4
Beta strandi16 – 19Combined sources4
Helixi21 – 37Combined sources17
Beta strandi38 – 44Combined sources7
Helixi47 – 54Combined sources8
Beta strandi59 – 61Combined sources3
Helixi62 – 84Combined sources23
Helixi85 – 87Combined sources3
Beta strandi91 – 96Combined sources6
Helixi99 – 102Combined sources4
Helixi104 – 119Combined sources16
Beta strandi123 – 128Combined sources6
Turni130 – 132Combined sources3
Helixi135 – 138Combined sources4
Turni140 – 142Combined sources3
Helixi143 – 152Combined sources10
Beta strandi156 – 164Combined sources9
Beta strandi168 – 170Combined sources3
Turni172 – 174Combined sources3
Beta strandi183 – 185Combined sources3
Helixi189 – 191Combined sources3
Helixi206 – 219Combined sources14
Beta strandi223 – 231Combined sources9
Helixi233 – 240Combined sources8
Beta strandi246 – 250Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AKOX-ray2.20A/B/C/D1-251[»]
ProteinModelPortaliQ9PJ29.
SMRiQ9PJ29.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9PJ29.

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT. Bacteria.
COG0263. LUCA.
HOGENOMiHOG000246368.
KOiK00931.
OMAiHDYGMRC.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PJ29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRIVVKVGS HVISEENTLS FERLKNLVAF LAKLMEKYEV ILVTSAAISA
60 70 80 90 100
GHTKLDIDRK NLINKQVLAA IGQPFLISVY NELLAKFNKL GGQILLTGKD
110 120 130 140 150
FDSRKATKHA KNAIDMMINL GILPIINEND ATAIEEIVFG DNDSLSAYAT
160 170 180 190 200
HFFDADLLVI LSDIDGFYDK NPSEFSDAKR LEKITHIKEE WLQATIKTGS
210 220 230 240 250
EHGTGGIVTK LKAAKFLLEH NKKMFLASGF DLSVAKTFLL EDKQIGGTLF

E
Length:251
Mass (Da):27,843
Last modified:October 1, 2000 - v1
Checksum:iD07802E92023A241
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34268.1.
PIRiA81426.
RefSeqiWP_002851769.1. NC_002163.1.
YP_002343557.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL34268; CAL34268; Cj0097.
GeneIDi904426.
KEGGicje:Cj0097.
PATRICi20057121. VBICamJej33762_0095.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34268.1.
PIRiA81426.
RefSeqiWP_002851769.1. NC_002163.1.
YP_002343557.1. NC_002163.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AKOX-ray2.20A/B/C/D1-251[»]
ProteinModelPortaliQ9PJ29.
SMRiQ9PJ29.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9PJ29. 9 interactors.
STRINGi192222.Cj0097.

Proteomic databases

PaxDbiQ9PJ29.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL34268; CAL34268; Cj0097.
GeneIDi904426.
KEGGicje:Cj0097.
PATRICi20057121. VBICamJej33762_0095.

Phylogenomic databases

eggNOGiENOG4105CGT. Bacteria.
COG0263. LUCA.
HOGENOMiHOG000246368.
KOiK00931.
OMAiHDYGMRC.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
BioCyciCJEJ192222:GJTS-90-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ9PJ29.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPROB_CAMJE
AccessioniPrimary (citable) accession number: Q9PJ29
Secondary accession number(s): Q0PC40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.