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Protein

Glutamate 5-kinase

Gene

proB

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei7 – 71ATPUniRule annotation
Binding sitei45 – 451SubstrateUniRule annotation
Binding sitei130 – 1301SubstrateUniRule annotation
Binding sitei142 – 1421Substrate; via amide nitrogenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi162 – 1632ATP
Nucleotide bindingi168 – 1736ATP
Nucleotide bindingi204 – 2107ATP

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate 5-kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-proline biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-93-MONOMER.
UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:Cj0097
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000000799: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Glutamate 5-kinasePRO_0000109656Add
BLAST

Interactioni

Protein-protein interaction databases

IntActiQ9PJ29. 9 interactions.
STRINGi192222.Cj0097.

Structurei

Secondary structure

1
251
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86Combined sources
Helixi10 – 134Combined sources
Beta strandi16 – 194Combined sources
Helixi21 – 3717Combined sources
Beta strandi38 – 447Combined sources
Helixi47 – 548Combined sources
Beta strandi59 – 613Combined sources
Helixi62 – 8423Combined sources
Helixi85 – 873Combined sources
Beta strandi91 – 966Combined sources
Helixi99 – 1024Combined sources
Helixi104 – 11916Combined sources
Beta strandi123 – 1286Combined sources
Turni130 – 1323Combined sources
Helixi135 – 1384Combined sources
Turni140 – 1423Combined sources
Helixi143 – 15210Combined sources
Beta strandi156 – 1649Combined sources
Beta strandi168 – 1703Combined sources
Turni172 – 1743Combined sources
Beta strandi183 – 1853Combined sources
Helixi189 – 1913Combined sources
Helixi206 – 21914Combined sources
Beta strandi223 – 2319Combined sources
Helixi233 – 2408Combined sources
Beta strandi246 – 2505Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AKOX-ray2.20A/B/C/D1-251[»]
ProteinModelPortaliQ9PJ29.
SMRiQ9PJ29. Positions 2-251.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9PJ29.

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0263.
HOGENOMiHOG000246368.
KOiK00931.
OMAiALIICSD.
OrthoDBiEOG6PGK7G.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PJ29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRIVVKVGS HVISEENTLS FERLKNLVAF LAKLMEKYEV ILVTSAAISA
60 70 80 90 100
GHTKLDIDRK NLINKQVLAA IGQPFLISVY NELLAKFNKL GGQILLTGKD
110 120 130 140 150
FDSRKATKHA KNAIDMMINL GILPIINEND ATAIEEIVFG DNDSLSAYAT
160 170 180 190 200
HFFDADLLVI LSDIDGFYDK NPSEFSDAKR LEKITHIKEE WLQATIKTGS
210 220 230 240 250
EHGTGGIVTK LKAAKFLLEH NKKMFLASGF DLSVAKTFLL EDKQIGGTLF

E
Length:251
Mass (Da):27,843
Last modified:October 1, 2000 - v1
Checksum:iD07802E92023A241
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34268.1.
PIRiA81426.
RefSeqiYP_002343557.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL34268; CAL34268; Cj0097.
GeneIDi904426.
KEGGicje:Cj0097.
PATRICi20057121. VBICamJej33762_0095.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34268.1.
PIRiA81426.
RefSeqiYP_002343557.1. NC_002163.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AKOX-ray2.20A/B/C/D1-251[»]
ProteinModelPortaliQ9PJ29.
SMRiQ9PJ29. Positions 2-251.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9PJ29. 9 interactions.
STRINGi192222.Cj0097.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL34268; CAL34268; Cj0097.
GeneIDi904426.
KEGGicje:Cj0097.
PATRICi20057121. VBICamJej33762_0095.

Phylogenomic databases

eggNOGiCOG0263.
HOGENOMiHOG000246368.
KOiK00931.
OMAiALIICSD.
OrthoDBiEOG6PGK7G.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.
BioCyciCJEJ192222:GJTS-93-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ9PJ29.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB.
InterProiIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 11168.
  2. "Crystal structure of glutamate 5-kinase from Campylobacter jejuni."
    Gorman J., Shapiro L.
    Submitted (AUG-2005) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH ADP.

Entry informationi

Entry nameiPROB_CAMJE
AccessioniPrimary (citable) accession number: Q9PJ29
Secondary accession number(s): Q0PC40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: October 1, 2000
Last modified: January 7, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.